Enzymes and Inhibitors
Apexbio Technology LLC E.-COLI-DNA-LIGASE-200U
E coli DNA ligase is an enzyme used extensively in molecular biology research particularly within the fields of genetic engineering and biotechnology Originating from the bacterium Escherichia coli this enzyme plays a crucial role in DNA replication and repair by catalyzing the formation of phosphodiester bonds between the 3 -hydroxyl and 5 -phosphate ends of DNA fragments Its mechanism of action involves the joining of Okazaki fragments on the lagging strand during DNA replication as well as sealing nicks in the DNA backbone It exhibits high activity in in vitro applications typically within the nanomolar to low micromolar range E coli DNA ligase is commonly used in applications involving cell models animal studies and drug screening protocols where its ability to ligate DNA fragments is essential Experimental concentrations typically depend on the specific research design allowing for flexibility in various experimental setups
Medchemexpress LLC Ac-YVAD-AMC | 149231-65-2 | MFCD00171369 | 99.6% | 665.69 | 10 MG
Ac-YVAD-AMC is a synthetic tetrapeptide substrate and a caspase inhibitor. It is utilized in research for inhibiting bacteria-induced cell death within hypersensitive response (HR) cells.
- Cleaved by Caspase-1, releasing fluorogenic AMC.
Medchemexpress LLC Suc-AEPF-AMC | 142997-30-6 | 98.67% | 719.74 | 5 MG
Suc-AEPF-AMC is a peptide substrate of Pin1 and Par14 peptidyl prolyl isomerase. It is used in research for studying biological activity.
- Peptide substrate of Pin1 and Par14 peptidyl prolyl isomerase
- Suitable for research applications
Medchemexpress LLC Z-DEVD-AMC | 98.2% | 767.74 | 10 MG
Z-DEVD-AMC is a selective caspase-3 substrate that can be measured by fluorescence spectrometry. It serves as a fluorescence reference standard for AMC-based enzyme substrates, including AMC-based caspase substrates.
- Selective caspase-3 substrate
- Measured by fluorescence spectrometry
- Functions as a fluorescence reference standard for AMC-based enzyme substrates
- Purity of 98.2%
- Molecular weight of 767.74
- White to off-white solid appearance
- Research use only
- Excitation wavelength of 390
- Emission wavelength of 480
Medchemexpress LLC Boc-Glu-Lys-Lys-AMC | 73554-85-5 | 99.9% | 660.76 | 10 MG
Boc-Glu-Lys-Lys-AMC is a sensitive fluorogenic substrate for urokinase-activated plasmin. It is intended for research use only.
- Sensitive fluorogenic substrate
- For urokinase-activated plasmin
- Research use only