Enzymes and Inhibitors
Thermo Scientific™ E. coli DNA Ligase
Thermo Scientific™ E. coli DNA Ligase is an NAD-dependent DNA ligase. It catalizes formation of phosphodiester bonds between 5'-phospate and 3'-hydroxyl termini in double-stranded DNA.
Invitrogen™ Alexa Fluor™ 647-Poly-GT Peptide Substrate
A general AlexaFluor647-labeled peptide substrate consisting of a polymer of glutamate, and tyrosine that is used to monitor tyrosine kinase acitivty.
Thermo Scientific™ XbaI, HC (50 U/μL)
Cut at T^CTAGA sites with XbaI restriction enzyme, which functions best at 37°C in Tango buffer.
Invitrogen™ Topoisomerase I
Catalyzes removal of superhelical turns from covalently closed DNA by a transient breakage and rejoining of phosphodiester bonds
Thermo Scientific™ FastDigest SmiI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Invitrogen™ EnzChek™ Cellulase Substrate, blue fluorescent, 339/452
Mixed with enzyme to produce fluorescent reaction
Invitrogen™ CMAC, t-BOC-Leu-Met (7-Amino-4-Chloromethylcoumarin, t-BOC-L-Leucyl-L-Methionine amide)
Cleavage produces blue-fluorescence
Invitrogen™ TrueCut™ HiFi Cas9 Protein (1 μg/μL)
A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut™ HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.
Invitrogen™ ELF™ 97 Phosphatase Substrate (ELF™ 97 Phosphate), 0.2 μm Filtered
For use with microarray, flow cytometer and fluorescence microscope
Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase), 1 U/μL
Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA
Thermo Scientific™ AarI-AjiI-Bpu10I-ScaI-PasI Buffer (10X)
Optimize digestions with AarI, AjiI, Bpu10I, ScaI, and PasI restriction enzymes using this buffer.
Thermo Scientific™ Buffers for RNA Transctription and Post-Transcriptional Modifications
Thermo Scientific enzymes for in vitro transcription are designed for efficient and reliable RNA synthesis. These enzymes cover the entire synthesis workflow from template preparation to post-transcriptional modifications, ensuring optimal performance and high yields of synthesized RNA.
Thermo Scientific™ PvuII (10 U/μL)
The PvuII restriction enzyme recognizes CAG^CTG sites and cuts best at 37°C in G buffer.
Thermo Scientific™ FastDigest HincII
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.