Enzymes and Inhibitors
Carnabio Usa Inc ROCK1/10UG/CATALYTIC
ROCK1, Catalytic domain, Wild type, Amino Acid 1-477, NP_005397.1, Expressed in Insect (sf21); 10microg
Bioworld Macerozyme R-10, 5 g
Macerozyme R-10Macerating enzyme from Rhizopus sp. suitable for the isolation of plant cells and can be used in combination with cellulase.Specification:Used in plant protoplast preparation to digest cell wall prior to oranelle isolation.A multi-component enzyme mixture containing approximately: * Cellulase: 0.1 U/mg * Hemicellulase: 0.25 U/mg * Pectinase: 0.5 U/mgSource: Rhizopus sp.Solubility: Soluble in water
Bioworld Macerozyme R-10, 1 g
Macerozyme R-10Macerating enzyme from Rhizopus sp. suitable for the isolation of plant cells and can be used in combination with cellulase.Specification:Used in plant protoplast preparation to digest cell wall prior to oranelle isolation.A multi-component enzyme mixture containing approximately: * Cellulase: 0.1 U/mg * Hemicellulase: 0.25 U/mg * Pectinase: 0.5 U/mgSource: Rhizopus sp.Solubility: Soluble in water
Bioworld Lysozyme, 5 g
Lysozyme Enzyme used in purification protocols of genomic and plasmid DNA to lyse bacterial cells.Ultra Pure GradeSource: Hen Egg White (also called Hen Egg Lysozyme or HEL.)Activity(25 C): >20,000U/mgAlbumin: None detectedSalmonella: None detected
New England Biolabs, Inc. RNase H – 250 units
RNase H (Ribonuclease H ) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double-stranded DNA.
New England Biolabs, Inc. Klenow Fragment (3'-5' exo-) – 1000 units
Klenow Fragment (3' to 5' exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5' to 3' exonuclease activity and has mutations (D355A, E357A) which abolish the 3' to 5' exonuclease activity.
New England Biolabs, Inc. TaqI Methyltransferase – 1000 units
TaqI Methyltransferase modifies the adenine residue (N6) of the sequence TCGA.
New England Biolabs, Inc. Endoglycosidase Reaction Buffer Pack – 4 x 1 ml
The Endoglycosidase Reaction Buffer Pack contains four buffers: 10X Glycobuffer 2, 10X Glycobuffer 3, 10X Glycoprotein Denaturing Buffer and 10% NP-40, most commonly used in endoglycosidase reactions.
New England Biolabs, Inc. Exonuclease T – 1250 units
Exonuclease T (Exo T), also known as RNase T, is a single-stranded RNA or DNA specific nuclease that requires a free 3' terminus and removes nucleotides in the 3' to 5' direction. Exonuclease T can be used to generate blunt ends from RNA or DNA molecules that have 3 extensions.
New England Biolabs, Inc. E.coli DNA Ligase – 1000 units
E. coli DNA Ligase catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates. E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated.
New England Biolabs, Inc. RNase If – 5000 units
Ribonuclease If (RNase If) is an RNA endonuclease which will cleave at all RNA dinucleotide bonds leaving a 5'hydroxyl and 2',3' cyclic monophosphate. It has a preference for single-stranded RNA over double-stranded RNA. RNase If is a recombinant protein fusion of RNase I (from E. coli) and maltose-binding protein. It has identical activity to RNase I.
Athens Research And Technology Inc ELASTASE HU NEUTROPHIL 100UG
Elastase, Human Neutrophil: 100 UG; Salt-Free lyophilized solid.; MW 29,500; EC = 0.985; Activity: 20-22 units per mg protein; Purity: > =95% by SDS-PAGE. This native protein is prepared and purified from whole blood in the Athens Research & Tech lab.