Enzymes and Inhibitors
Thermo Scientific™ BglI (10 U/μL)
Cut at GCCNNNN^NGGC sites with BglI restriction enzyme, which performs best at 37°C in O buffer.
Thermo Scientific™ Eam1105I (AhdI) (10 U/μL)
Cut at GACNNN^NNGTC sites with Eam1105I (AhdI) restriction enzyme, which performs best at 37°C in its own unique buffer (Isoschizomers: AhdI, AspEI, BmeRI, DriI, EclHKI).
Invitrogen™ Gateway™ LR Clonase™ II Enzyme mix
Contains enzymes and buffer in a single mix to enable convenient ten-microliter reaction set-up with fewer pipetting steps.
Thermo Scientific™ PvuII (10 U/μL)
The PvuII restriction enzyme recognizes CAG^CTG sites and cuts best at 37°C in G buffer.
Thermo Scientific™ SmaI (10 U/μL)
Cut at CCC^GGG sites with SmaI restriction enzyme, which performs best at 30°C in Tango buffer (Isoschizomers: TspMI, XmaCI, XmaI).
Invitrogen™ L.b. Cas12a Nuclease
L.b. Cas12a Nuclease for simple, quick and specific analysis of your samples.
Thermo Scientific™ AatII (10 U/μL)
Cut at GACGT^C sites with AatII restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: ZraI).
Thermo Scientific™ ApaI (10 U/μL)
Cut at GGGCC^C sites with ApaI restriction enzyme, which functions best at 37°C in B buffer (Isoschizomers: PspOMI).
Thermo Scientific™ Bsh1236I (BstUI) (10 U/μL)
Cut at CG^CG sites with Bsh1236I (BstUI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: AccII, BspFNI, BstFNI, BstUI, MvnI).
Thermo Scientific™ Bsp1407I (BsrGI) (10 U/μL)
Cut at T^GTACA sites with Bsp1407I (BsrGI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BsrGI, BstAUI).
Thermo Scientific™ SacI (10 U/μL)
Cut at GAGCT^C sites with SacI restriction enzyme, which performs best at 37°C in its own unique buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).
Thermo Scientific™ HhaI (10 U/μL)
Cut at GCG^C sites with HhaI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AspLEI, BstHHI, CfoI, HinP1I, HspAI).
Invitrogen™ Anza™ 17 KpnI
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Thermo Scientific™ FastDigest BspTI
Cut at C^TTAAG sites with Thermo Scientific™ FastDigest™ AflII (BspTI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.