Enzymes and Inhibitors
Supply Solutions Liberase™ TL Research Grade, pkg of 10 mg (2x5 mg)
The most commonly used method for decellularization procedures, such as tissue dissociation and cell harvesting, is based on the use of proteolytic enzymes. Proteases disrupt the extracellular matrix of tissue to allow the release of individual cells or the harvesting of cultured cells for transfer. The goal of cell isolation is to maximize the yield of dissociated cells that are viable and functionally active.
LGC GENOMICS LLC (Lucigen) NxGen T4 DNA Ligase (Low Concentration)A workhorse, high quality DNA ligase for joining blunt or cohesive ends of dsDNA or dsRNA.
NxGen T4 DNA Ligase (Low Concentration)A workhorse, high quality DNA ligase for joining blunt or cohesive ends of dsDNA or dsRNA.Reliably join double-stranded nucleic acid chains.The low concentration (LC) NxGen T4 DNA Ligase Kit includes T4 DNA Ligase at 2 U/¿L and 10X T4 DNA ligase Buffer. The high concentration(HC) NxGen T4 DNA Ligase Kit includes NxGen T4 DNA Ligase at 10 U/¿L and both 2X Rapid Ligation Buffer and 10X T4 DNA ligase Buffer.
Medchemexpress LLC D-luciferin potassium | 115144-35-9 | 100.0% | 318.41 g·mol^-1 | C11H7KN2O3S2 | 1G
D-Luciferin potassium is the water-soluble potassium salt of D-luciferin, the natural substrate for firefly luciferase. It produces yellow-green bioluminescence upon ATP-dependent oxidation and is used for biochemical luciferase assays, cell-based reporter assays, and in vivo imaging. The compound is supplied as a solid powder suitable for preparation of aqueous solutions for experimental use.
- Water-soluble potassium salt for easy aqueous formulation.
- Suitable for bioluminescent luciferase assays and imaging.
- High analytical purity (manufacturer lists 99.97%).
- Molecular weight 318.41 g·mol⁻¹ and formula C11H7KN2O3S2.
- Available in multiple pack sizes including 1 g for laboratory use.
Medchemexpress LLC Z-leu-leu-glu-AMC | 348086-66-8 | MFCD01074989 | 99.5% | 664.75 g/mol | C35H44N4O9 | 1MG
Z-Leu-Leu-Glu-AMC is a fluorogenic tripeptide substrate used to measure post-acidic (caspase-like) hydrolytic activity of the 20S proteasome. Proteolytic cleavage releases 7-amino-4-methylcoumarin (AMC), producing a fluorescent signal suitable for kinetic assays, inhibitor screening, and proteasome activity profiling in biochemical research.
- High purity (≈99.5%) for reliable assay performance.
- Releases AMC for sensitive fluorometric detection.
- Suitable for 20S/26S proteasome activity and inhibitor assays.
- Available as solid and 10 mM DMSO solution formats.
- Supplied in small research pack sizes for convenient use.
New England Biolabs, Inc. TspMI – 200 units
A restriction endonuclease that recognizes the sequence C^CCGG_G.
Biotium Aquaphile™ Coelenterazine Native, lyophilized solid
Aquaphile coelenterazine native is a bioluminescence substrate in a special formulation format that is readily dissolved in water or PBS buffer for in vivo use.Properties: Yellow solid soluble in water or PBS buffer; Store at -20°C and protect from light; C26H21N3O3; MW: 423; [55779-48-1]
Sigma Aldrich Fine Chemicals Biosciences Lactoperoxidase from bovine milk|9003-99-0 | MFCD00131484 | 1mg
Lactoperoxidase from bovine milkPurity: MW: | 9003-99-0 | MFCD00131484 | 1mg
Sigma Aldrich Fine Chemicals Biosciences Proteinase K from Tritirachium album 3.0 unit/mg solid, lyophilized powder | 39450-01-6 | MFCD00132129 | 1g
Proteinase K from Tritirachium album 3.0 unit/mg solid, lyophilized powder | 39450-01-6 | MFCD00132129 | 1g
Sigma Aldrich Fine Chemicals Biosciences ADENOSINE DEAMINASE (ADA) 2ML
Adenosine Deaminase (ADA) from calf intestine 2mL
LGC GENOMICS LLC (Lucigen) Riboguard RNase Inhibitor, 2500 units @ 40 U/LRNA is precious. Protect it with this high quality, effective RNase inhibitor. Manufactured under ISO-13485.
RiboGuard™ RNase Inhibitor is a recombinant protein that is the best defense against common RNases, including RNase A, RNase B, and RNase C.
LGC GENOMICS LLC (Lucigen) Baseline-ZERO DNase, 5000 UDigest large or small DNA molecules better than standard DNase I.
Baseline-ZERO™ DNase digests dsDNA and ssDNA into mononucleotides more effectively than the commonly used bovine pancreatic DNase I.
LGC GENOMICS LLC (Lucigen) Plasmid-Safe ATP-Dependent DNase, 10,000 U @ 10 U/LDigest unwanted contaminating linear double-stranded DNA, without harming circular double-stranded DNA such as plasmids and fosmids.
Plasmid-Safe™ ATP-Dependent DNase selectively removes contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC clones or vector preparations.
Sigma Aldrich Fine Chemicals Biosciences Neuraminidase from Vibrio cholerae, 2 units
Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.
Takara Bio Recombinant DNase I (RNase-free), 1,000 Units
Recombinant DNase I (RNase-free) is an endonuclease that catalyzes, to the same degree, the random degradation of both single- and double-stranded DNA and produces 5'-P terminal oligonucleotides. This enzyme does not exhibit protease activity. This enzyme is stable around its optimum neutral pH range and is suitable for RNA preparation at neutral pH. 1000 Units
Athens Research And Technology Inc ELASTASE HU NEUTROPHIL 1MG
Elastase, Human Neutrophil: 1 MG; Salt-Free lyophilized solid.; MW 29,500; EC = 0.985; Activity: 20-22 units per mg protein; Purity: > =95% by SDS-PAGE. This native protein is prepared and purified from whole blood in the Athens Research & Technology laboratory. Molecular Biology Reagent