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Z-Leu-Leu-Glu-AMC is a fluorogenic peptide substrate for measuring postacidic-like (peptidylglutamyl-peptide hydrolyzing, PGPH) activity of the 20S proteasome. Proteolytic cleavage liberates the fluorescent 7-amino-4-methylcoumarin (AMC) reporter, enabling quantitative proteasome activity assays for research use.
Fluorogenic substrate that releases 7-amino-4-methylcoumarin (AMC) upon cleavage.
Designed to measure postacidic-like (PGPH) activity of the 20S proteasome.
Reported purity 99.5%.
Chemical formula C35H44N4O9.
Molecular weight 664.75 g·mol⁻¹.
White to off-white solid appearance.
Available in multiple pack sizes including 50 mg.
Storage: powder stable at -80°C for long-term and -20°C for short-term when sealed and protected from moisture.
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A fluorogenic substrate for vanin-1; AMC is released upon enzymatic cleavage by vanin-1 and its fluorescence can be used to quantify vanin-1 activity; ex/em = 340-360/440-460 nm, respectively
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cGAS TR-FRET Substrate Mix has been tested and formulated to work exclusively with Cayman’s cGAS TR-FRET Inhibitor Screening Assay Kit (Item No. 702120).
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Highly sensitive substrate for human and mouse neutrophil elastase (leukocyte elastase) and neutrophil proteinase 3 (PR-3, myeloblastin), but not cathepsin G nor chymotrypsin. This substrate can be used for in vitro assays with purified enzyme, or with biological fluids or conditioned medium. Hydrolysis of the substrate can be detected at 405-410 nm. Purity: ≥98%. Solubility: Soluble in DMSO (>20 mM) or methanol (1 mg/mL). Long Term Storage: -20°C.
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MW 975.5 Da. Fluorescent triglyceride analog useful for measuring cholesteryl ester transfer and protein-mediated triacylglycerol transport between plasma lipoproteins. Popular substrate for detections of lipoprotein lipase and hepatic triacylglycerol lipases.
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A substrate for pancreatic elastase; cleaved by elastase to release p-NA, which can be quantified by colorimetric detection at 405 nm as a measure of enzyme activity
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The HER2/CD340 protein is a dynamic tyrosine kinase that is essential in the neuregulin receptor complex and regulates microtubule dynamics Upon activation it triggers the MEMO1-RHOA-DIAPH1 pathway inhibits GSK3B and promotes the association of APC and CLASP2 on the cell membrane to achieve microtubule stabilization HER2/CD340 Protein Human (S310Y HEK293 His) is the recombinant human-derived HER2/CD340 protein expressed by HEK293 with C-10 His labeled tag and S310Y mutation
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MW 838.7 Da, Purity >97%. Ac-WEHD-AFC is a useful fluorogenic substrate for group I caspases (caspase-1, -4, and -5). Caspase activity can be quantified by fluorescent detection of free AFC that has an excitation maximum at 400 nm and emission maximum at 505 nm.