Enzymes and Inhibitors
New England Biolabs, Inc. Endonuclease VIII – 5000 units
Endonuclease VIII from E. coli acts as both an N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating an apurinic (AP site). The AP-lyase activity cleaves 3' and 5' to the AP site leaving a 5' phosphate and a 3' phosphate. Damaged bases recognized and removed by Endonuclease VIII include urea, 5, 6- dihydroxythymine, thymine glycol, 5-hydroxy-5- methylhydantoin, uracil glycol, 6-hydroxy-5, 6-dihydrothymine and methyltartronylurea. While Endonuclease VIII is similar to Endonuclease III, Endonuclease VIII has and lyase activity while Endonuclease III has only lyase activity.
New England Biolabs, Inc. RsaI – 5000 units
A restriction endonuclease that recognizes the sequence GT_^AC.
New England Biolabs, Inc. HpaII – 10000 units
A restriction endonuclease that recognizes the sequence C^CG_G.
New England Biolabs, Inc. DdeI – 1000 units
A restriction endonuclease that recognizes the sequence C^TNA_G.
New England Biolabs, Inc. BfaI – 2500 units
A restriction endonuclease that recognizes the sequence C^TA_G.
New England Biolabs, Inc. Tsp45I – 200 units
A restriction endonuclease that recognizes the sequence ^GTSAC_.
New England Biolabs, Inc. Hpy188I – 5000 units
A restriction endonuclease that recognizes the sequence TC_N^GA.
New England Biolabs, Inc. HpyCH4V – 500 units
A restriction endonuclease that recognizes the sequence TG_^CA.
New England Biolabs, Inc. BfuAI – 250 units
A restriction endonuclease that recognizes the sequence ACCTGCNNNN^NNNN_.
New England Biolabs, Inc. BmgBI – 500 units
A restriction endonuclease that recognizes the sequence CAC_^GTC.
Chem-Impex International, Inc. Pepsin 1:10000 | 9001-75-6 | MFCD00081840 | 25G
Pepsin 1:10000, 9001-75-6, MFCD00081840, 25G
Chem-Impex International, Inc. a-Chymotrypsin from bovine pancreas | 9004-07-3 | MFCD00130481 | 1G
a-Chymotrypsin from bovine pancreas, 9004-07-3, MFCD00130481, 1G
AdipoGen 5(6)-CFDA N-succinimidyl ester (50 mg)
5(6)-CFDA N-succinimidyl ester (50 mg), Adipogen Life Sciences. Chemical. CAS: 150347-59-4. Formula: C29H19NO11. MW: 557.46. Synthetic. 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is a useful fluorescent tracer that can passivley diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.
Research Products International Corp X-GAL and IPTG Ready to Use Non-Toxic Solution 10mg/ml, 100 Milligrams
Stable solution of X-GAL and IPTG. Does not contain DMSO or DMF. Solution concentration is 10 mg/ml X-Gal and 8 mg/ml IPTG.
Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. WARNING! This product can expose you to 1-Methyl-2-Pyrrolidon, a chemical which is known to the State of California to cause developmental harm. For more information go to www.P65Warnings.ca.gov.