Enzymes and Inhibitors
New England Biolabs, Inc. Exonuclease T – 1250 units
Exonuclease T (Exo T), also known as RNase T, is a single-stranded RNA or DNA specific nuclease that requires a free 3' terminus and removes nucleotides in the 3' to 5' direction. Exonuclease T can be used to generate blunt ends from RNA or DNA molecules that have 3 extensions.
New England Biolabs, Inc. RNase If – 5000 units
Ribonuclease If (RNase If) is an RNA endonuclease which will cleave at all RNA dinucleotide bonds leaving a 5'hydroxyl and 2',3' cyclic monophosphate. It has a preference for single-stranded RNA over double-stranded RNA. RNase If is a recombinant protein fusion of RNase I (from E. coli) and maltose-binding protein. It has identical activity to RNase I.
Cayman Chemical Monoacylglycerol Lipase Block
Peptide Sequence: human monoacylglycerol lipase blocking peptide amino acids 1-14 (MPEESSPRRTPQSI) · To be used in conjunction with Cayman’s Monoacylglycerol Lipase Polyclonal Antibody (Item No. 100035) to block protein-antibody complex formation during analysis for monoacylglycerol lipase.
New England Biolabs, Inc. Taq DNA Ligase – 2000 units
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands. The strands to be ligated need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37 C - 75 C).
Aniara Diagnostica LLC HUMAN THROMBIN 10X100 NIH
This item MFG by HYPHEN BioMed and Dis. by Aniara Diagnostics. Highly purified human Thrombin (MW=35,000 Da), mainly in the alpha form, lyophilized with PEG, NaCl, Glycine, BSA, Calcium and stabilizers. Specific activity > 2000 NIH/mg. For in vitro use only. 10 vials x 100 NIH.
Sigma Aldrich Fine Chemicals Biosciences Thrombin from bovine plasma | 9002-04-4 | MFCD00166053 | 100 units
Thrombin from bovine plasma | Mol Wt: ~33 kDa | 9002-04-4 | MFCD00166053 | 100 units
New England Biolabs, Inc. NEBuffer Set (EcoRI, DpnII) – 1.25 ml of each
New England Biolabs provides 10X NEBuffer with each restriction endonuclease to assure optimal (100%) activity. Set contains two 1.25ml vials of each unique NEBuffer for the enzymes EcoRI and DpnII. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer. 1X NEBuffer EcoRI 100 mM Tris-HCl 50 mM NaCl 10 mM MgCl2 0.025% Triton X-100 (pH 7.5 @ 25C) 1X NEBuffer DpnII 50 mM Bis-Tris-HCl 100 mM NaCl 10 mM MgCl2 1 mM DTT (pH 6 @ 25C)
Agilent Technologies PFUTURBO DNA POLYMERASEPFUTUR
PfuTurbo DNA Polymerase, 500U (200 rxn), Contents: PfuTurbo DNA polymerase 10X cloned Pfu buffer, Applications: High performance PCR, For high fidelity PCR and primer-extension reactions, PCR cloning and generating blunt-end amplification p
American Research Products Inc ASPARTATE TRANSAMINASE AST KIT
5000265273 ASPARTATE TRANSAMINASE AST KIT
Integrated DNA Technologies Inc Alt-R S.p. HiFi Cas9 Nuclease V3, 500 µg
Alt-R S.p. HiFi Cas9 Nuclease V3, 500 µg
INTACT GENOMICS INC T4 DNA Ligase 100,000 Units (2000 units/µl)
Intact Genomics T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. This enzyme joins DNA fragments with either cohesive or blunt termini and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids (1).Applications• Cloning of restriction enzyme generated DNA fragments• Cloning of PCR products• Next-gen library preparation• Joining linkers and adapters to cohesive or blunt-ended DNA• Nick repair in duplex DNA, RNA or DNA/RNA hybrids• Self-circularization of linear DNA
New England Biolabs, Inc. RNase H – 250 units
RNase H (Ribonuclease H ) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double-stranded DNA.