Modifying Enzyme Buffers
TARGETMOL CHEMICALS INC PROTEINASE K 500MG
Also available in 100 mg and bulk. Please contact Fisher for quotes. Proteinase K (proteinase K for tritirachium album) is a nonspecific serine protease that is useful for general digestion of proteins. It is active in the presence of SDS or urea and over a wide range of pH (4-12) salt concentrations and temperatures. purity: 98%
Sigma Aldrich Fine Chemicals Biosciences Proteinase K from Tritirac5MG
Tritirac Proteinase K from Tritirac5MG
Gold Biotechnology Inc Proteinase K RNase DNase free
Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins It cleaves at the carboxylic acid side of aliphatic aromatic or hydrophobic amino acids Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids Proteinase K is also useful for helping to characterize enzymes due to its cleavage specificity This enzyme was designated proteinase K because of its ability to hydrolyze keratin Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH The isoelectric point of proteinase K is 8 9
Zymo Research Corporation R-Zymolyase (1000 Units Lyophilized) w/ Storage Buffer (500 µl)
This special formula of enzymes is specially manufactured for yeast genomic DNA preparations. Our liquid Zymolyase is prepared from Arthrobacter luteus. The primary yeast lytic activity is 1,3-glucan laminaripentaohydrolase. It hydrolyzes glucose polymers at the is -1,3-glucan linkages releasing laminaripentaose as the principal product. This formula may be used as Zymolyase as it is, but be cautious that it contains RNase A.
Gold Biotechnology Inc Proteinase K Solution
Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins It cleaves at the carboxylic acid side of aliphatic aromatic or hydrophobic amino acids Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids Proteinase K is also useful for helping to characterize enzymes due to its cleavage specificity This enzyme was designated proteinase K because of its ability to hydrolyze keratin Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH The isoelectric point of proteinase K is 8 9
Apexbio Technology LLC Phosphatase Inhibitor Cocktail 2 (100X in ddH2O) 100x1ml
Many cellular processes are regulated by reversible phosphorylation of proteins and approximately 30% of proteins may become phosphorylated during their lifetime Cellular crude extracts contain numerous endogenous enzymes such as phosphatases and proteases which can degrade and modify proteins present in the extract Addition of inhibitors for these enzymes is the optimal approach for maximizing intact protein yield Phosphatase Inhibitor Cocktail 2 inhibits tyrosine protein phosphatases acid phosphatases and alkaline phosphatases Phosphatase Inhibitor Cocktail 2 has been optimized and tested in cell extracts from multiple animal tissues This Phosphatase Inhibitor Cocktail 2 contains Sodium orthovanadate Sodium molybdate Sodium tartrate Imidazole and Sodium Fluoride It is supplied as a ready-to-use solution in ddH2O
Apexbio Technology LLC Phosphatase Inhibitor Cocktail 3 (100X in DMSO) 100x1ml
Numerous cellular processes are regulated by reversible protein phosphorylation with approximately 30% of proteins being phosphorylated during their lifespan Crude cell extracts contain various endogenous enzymes such as phosphatases and proteases which can degrade and alter proteins in the extracts Therefore the optimal method to increase the yield of intact proteins is to add inhibitors targeting these enzymes Phosphatase Inhibitor Cocktail 3 inhibits alkaline phosphatase L-isozymes and serine/threonine protein phosphatases such as protein phosphatase 1 and 2A (PP1 and PP2A) Phosphatase Inhibitor Cocktail 3 has been optimized and tested in cell extracts from various animal tissues Phosphatase Inhibitor Cocktail 3 can replace Phosphatase Inhibitor Cocktail 1 as the two cocktails have equivalent activities Phosphatase Inhibitor Cocktail 3 contains Cantharidin Bromotetramisole and Calyculin A This Phosphatase Inhibitor Cocktail is supplied as a ready-to-use solution in DMSO
Medchemexpress LLC Casein kinase inhibitor A86 | 2079069-01-3 | 99.3% | 344.43 g/mol | C18H25FN6 | 10MM 1ML
Casein kinase inhibitor A86 is a potent small-molecule inhibitor of casein kinase 1α (CK1α) with reported activity against CDK7 and CDK9. Supplied as a 10 mM solution in DMSO, it is intended for biochemical and cell-based research applications, including studies of apoptosis and leukemia-related signaling pathways.
- Potent CK1α inhibitor with activity against CDK7 and CDK9.
- Supplied as a 10 mM solution in DMSO for ready use in assays.
- High purity suitable for research: 99.3%.
- Molecular formula C18H25FN6; molecular weight 344.43 g/mol.
- Volume 1 mL, convenient for small-scale experiments.
- For research use only; not for human therapeutic applications.
Revvity Health Sciences Inc HTRF Enzymatic Kinase Buffer (5X), 10 mL
HTRF Enzymatic Kinase Buffer (5X), 10 mL
Medchemexpress LLC Casein Kinase 1 alpha Antibody (YA564) | Affinity purified | Predicted: 39 kDa, Observed: 34 kDa | 10 UL
Casein Kinase 1 alpha Antibody (YA564) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting Casein Kinase 1 alpha. It is for research use only.
- Host: Rabbit
- Clonality: Recombinant, monoclonal
- Species reactivity: Human, rat
- Immunogen: Synthetic peptide corresponding to human Casein Kinase 1 alpha. The exact sequence is proprietary to MCE.
- Sensitivity: Endogenous
- Conjugation: Non-conjugated
- Modification: Unmodified
- Isotype: IgG
- Appearance: Liquid
- Formulation: Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide.
- Storage and stability: Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
- Shipping: Shipping with blue ice.