Modifying Enzymes
Enzo Life Sciences Neutrophil elastase (human), (purified) (100 µg)
Functions in inflammation. Can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α , and α 1- antichymotrypsin. Because of its potential roles in inflammatory diseases such as emphysema and cystic fibrosis, it is a target for drug discovery. Alternative name: Leukocyte elastase. LE. Purity: ≥95% (SDS-PAGE). Although this is in highly purified form, and the. Source material tested negative for HbsAg, anti-HBc, anti-HCV, and anti-HIV-1 and -2, handle this product with all due precautions. Source: Isolated from human neutrophils. Active enzyme. Use/Stability: This enzyme is stable when stored under the above conditions. Procedures such as dilution of the enzyme followed by refreezing could lead to loss of activity. UniProt: P08246. Handling: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C. Long Term Storage: -20°C
Enzo Life Sciences Proteinase K (5 mg) CAS: 39450-01-6
PCR grade proteinase K used for molecular biology applications. ENZO Proteinase K is specifically designed for use in the pretreatment of formalin-fixed tissue and paraformaldehyde-fixed cells in preparation for in situ hybridization and detection studies. Formulation: Lyophilized. Source: Pichia pastoris UniProt: P06873. Long Term Storage: +4°C
Diagenode Inc Tagmentase TN5 TRN Unloaded, 10 μl, Hyperactive Tn5 transposase, Mg++ dependent, 19-bp ME DNA sequence required, 2 mg/ml protein concentration, 53.3 kDa molecular weight
Diagenode Tagmentase (Tn5 transposase) is a hyperactive enzyme designed for a wide range of molecular biology applications. It enables an efficient "cut and paste" tagmentation reaction, allowing simultaneous DNA cleavage and the insertion of specific oligonucleotides or sequencing adapters. As an unloaded variant, it provides researchers with the flexibility to customize the enzyme with oligonucleotides tailored to their specific experimental needs. This Tn5 transposase is essential for various Next-Generation Sequencing workflows.
- Enzyme type: Hyperactive Tn5 transposase
- Loading status: Unloaded
- Function: Catalyzes "cut and paste" tagmentation
- Activity requirement: Mg++ dependent
- Optimal pH: 7.5-8.0
- Optimal temperature: 37-55 °C
- Storage conditions: Store at -20 °C
- Protein concentration: 2 mg/ml
- Volume: 10 μl
Worthington Biochemical Corporation Miccrococal Nuclease, 15ku
Chromatographically purified to be essentially homogeneous chromatographically and electrophoretically (SDS-PAGE). A lyophilized powder.
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline-Agarose from calf intestine ammonium sulfate suspension | MFCD00131852 | 100UN
Phosphatase, Alkaline-Agarose from calf intestine ammonium sulfate suspension | MFCD00131852 | 100UN
Sigma Aldrich Fine Chemicals Biosciences Lambda protein phosphatase liquid, Bacteriophage Lambda, recombinant, expressed in E. coli | 401941-75-1 | MFCD00243301 | 20KU
Lambda protein phosphatase liquid, Bacteriophage Lambda, recombinant, expressed in E. coli | 401941-75-1 | MFCD00243301 | 20KU
ACROBiosystems GENIUS™Nuclease DMF Filed
GENIUS™Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. GENIUS™Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form ( single stranded, double stranded, linear, circular and supercoiled ). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5‘- phosphorylated oligonucleotides of 3-8 bases in length.
ACROBiosystems Endoglycosidase H (Endo H) is a recombinant glycosidase cloned from Streptomyces plicatus and overexpressed in E. coli. Endo H cleaves the chitobiose core of high-mannose oligosaccharides and a limited number of hybrid oligosaccharides from
Endoglycosidase H is a recombinant glycosidase which cleaves within the chitobiose core of high mannose and some hybrid oligosaccharides from N-linked glycoproteins. It is a recombinant glycosidase cloned from Streptomyces plicatus and overexpressed in E. coli.
ACROBiosystems The enzyme hydrolyzes the β-1,4 linkage between the core GlcNAc residues in the Fc-glycan, leaving the innermost GlcNAc intact on the Fc. It is derived from Streptococcus pyogenes and expressed in E. coli. The enzyme contains a His-tag and
EndoS is an endoglycosidase from Streptococcus pyogenes that specifically hydrolyzes N-linked glycans in the Fc-region of native IgG. It specifically deglycosylates the conserved N-glycans on the Fc region of IgG by hydrolyzing the chitobiose core (at the beta -1,4 linkage between the two N-acetylglucosamines) of the N-glycans, cleavage leave one GlcNAc residue remaining attached to the asparagine residue on the peptide backbone.
AG Scientific Inc RNase A, 500 MG
RNase stands for ribonuclease, an RNA digesting enzyme. RNase consists of 124 amino acids in one polypeptide chain with 4 disulphide linkages. Ribonucleases can be divided into endoribonucleases and exoribonucleases. RNases have important roles in RNA degradation and turnover in all organisms. Ribonuclease enzyme can unwind the RNA helix by complexing with single-stranded RNA. RNase A is an endoribonuclease with functions in RNA metabolism and regulation of gene expression. It was found to play roles in diseases such as autoimmune diseases, renal insufficiencies and pancreas disorder.CAS Number: 9001-99-4Chemical Formula: C9H14N4O3Storage Temperature: -20CResearch or further manufacturing use only, not for food or drug use.
AG Scientific Inc RNase A, 1 G
RNase stands for ribonuclease, an RNA digesting enzyme. RNase consists of 124 amino acids in one polypeptide chain with 4 disulphide linkages. Ribonucleases can be divided into endoribonucleases and exoribonucleases. RNases have important roles in RNA degradation and turnover in all organisms. Ribonuclease enzyme can unwind the RNA helix by complexing with single-stranded RNA. RNase A is an endoribonuclease with functions in RNA metabolism and regulation of gene expression. It was found to play roles in diseases such as autoimmune diseases, renal insufficiencies and pancreas disorder.CAS Number: 9001-99-4Chemical Formula: C9H14N4O3Storage Temperature: -20CResearch or further manufacturing use only, not for food or drug use.
Worthington Biochemical Corporation Deoxyribonuclease I, 25mg
Partially purified. A lyophilized powder. Store at 2-8°C. Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
Sigma Aldrich Fine Chemicals Biosciences T4 DNA Ligase recombinant
T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3-hydroxyl- and 5-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.ContentsT4 DNA Ligase supplied with 10x concentrated ligation buffer that includes ATP.