Modifying Enzymes
Bioworld DNase I (Bovine Pancreas), 100 KU
DNase I DNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA in the presence of divalent cations, producing 3'-OH oligonucleotides. Mg+2 in the reaction solution causes the enzyme to produce nicks in double-stranded DNA, while in the presence on Mn+2, DNase I cleaves both strands of the DNA. DNase I is useful in nick translation for introducing single-stranded nicks that serve as primer sites for initiation of DNA synthesis and for cloning random DNA fragments by cleaving double-stranded DNA. It is also used in purifying RNA from cells or transcription mixtures. Specifications:CAS: 9003-98-9EC: 232-667-0Source: Bovine pancreasActivity: ≥10000 U/mg.Ultra Pure GradeOur DNase I is guaranteed free of RNase contaminants.
Zymo Research Corporation Zymolyase Ultra 10000 U
Digestion of yeast and fungal cell walls is necessary for many experimental procedures. Lytic enzymes like Zymolyase Lyticase Glucanase or Chitinase are routinely used for digestion of cell walls. Distinguished from conventional lytic enzymes Zymolyase Ultra is a novel formulation of enzyme that is optimized for efficient yeast cell wall digestion. It contains enzymes that can degrade several different components of yeast cell walls. It is extensively purified using a novel DNA/RNA removal technology that results in extremely low nucleic acid contamination. It is suitable for qPCR and sequencing-based assays where low DNA/RNA contamination is critical.
LGC GENOMICS LLC (Lucigen) EpiScript RNase H- Reverse Transcriptase, 25,000 units @ 200 U/LAn optimised reverse transcriptase system for the production of full-length cDNA.
EpiScript™ Reverse Transcriptase an alternative to SuperScript II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with reduced RNase H activityProduce full-length cDNA with RNAse H-mutantAchieve higher specificity at elevated temperatures, up to 55 CKit Contents: EpiScript RNase H- Reverse Transcriptase, 10X Reaction Buffer, 100 mM DTT.
New England Biolabs, Inc. T7 RNA Polymerase – 25000 units
Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the T7 phage promoters. The 99 KD enzyme catalyzes in vitro RNA synthesis from a cloned DNA sequence under the T7 promoters. RNA produced using the T7 RNA Polymerase is suitable for many applications in research and biotechnology. Reaction Conditions 1X RNAPol Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP, and DNA template containing the T7 RNA Polymerase promoter. Incubate at 37°C.
New England Biolabs, Inc. T4 DNA Ligase – 20,000 units – 2,000,000 units/ml
The industry standard for performance and quality. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.
- Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA
- Joins blunt and cohesive end termini as well as repair single stranded nicks in duplex DNA
- T4 DNA Ligase variants: Salt-T4, Hi-T4, Instant Sticky-end Ligase MM, Blunt/TA Ligase MM
Athens Research And Technology Inc CATALASE HU ERYTHROCYTE 1MG
Catalase, Human Erythrocyte: 1 MG; Frozen in 50 mM Tris-HCl, pH 8.0; MW 244,000; Extinction Coefficient=1.39; Purity: > =95% by SDS-PAGE; Activity: > 50,000 u/mg protein. pH 7.0. Prepared from whole blood in the Athens Research & Tech lab.
Bioworld alpha-Chymotrypsin, 250 mg
Specification:Grade: USP gradeForm: White crystalline powderChymotrypsin acitvity (dry basis): 1000 U/mgTrypsin: 1%Residue after ignition: 2.5%Loss on drying: 5%Solubility (water, 10,000 U/ml): Pass
Enzo Life Sciences Matrix metalloproteinase (MMP) multipack-1 (1 Kit)
The MMP multipack-1 contains 10µg each of five highly active recombinant MMP catalytic domains: MMP-1, MMP-2, MMP-8, MMP-9, and MMP-13. Contents: Contains 10µg of each enzyme, provided in a screw-cap microfuge tube. MMP-1 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE180), MMP-2 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE237), MMP-8 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE255), MMP-9 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE360), MMP-13 (catalytic domain) (human), (recombinant) (Prod. N. BML-S24). Application: FUNC, HTS. Long Term Storage: -80°C
Cayman Chemical Precelys 24 User MaIntena 1ea
This kit provides Precellys 24 users with all of the necessary parts to properly maintain their instrument. EAch kit contains: 1 x Precellys 24 White Blocking Plate (2 mL) (Item No. 10422) 2 x Precellys 24 Vacuum Joint (toric joint) (Item No. 10011148) 1 x Precellys 24 Containment Seal (Item No. 10011149)] 1 x Precellys 24 Anti-Rotation Tubing Kit - (Item No. 10011162) [Bertin Catalog S000688-PR240-A.0; Previously SP03119-810-NC011.0]
Worthington Biochemical Corporation Phosphatase, Alkaline, Purified 5mg
Phosphatase, Alkaline, Purified 5mg
Enzo Life Sciences MMP-8 (catalytic domain) (human), (recombinant) (10 µg)
Alternative name: Matrix metalloproteinase 8, Neutrophil collagenase, Collagenase 2. Purity: ≥90% (SDS-PAGE). Formulation: Liquid. In 50mM TRIS, 5mM CaCl2, 300mM NaCl, 20µM ZnCl2, 0.5% Brij-35, and 30% glycerol. Source: Produced in E. coli. Active Matrix Metalloproteinase-8 (MMP-8, neutrophil collagenase, collagenase-2) catalytic domain from human cDNA. The enzyme consists of the catalytic domain of human MMP-8 (Phe99-Gln269, NM_002424) with a C-terminal purification tag. This represents a naturally-occurring active form of MMP-8 which lacks the C-terminal hemopexin domain1. MMPs lacking this domain cannot cleave native collagens; however, activity toward other targets such as gelatin, casein, or peptide substrates is unaffected. Long Term Storage: -80°C.
New England Biolabs, Inc. EpiMark Hot Start Taq DNA Polymerase – 100 reactions
EpiMark Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and a temperature sensitive, aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of reactions at room temperature. An advantage of the aptamer-based hot start mechanism is that it does not require a separate high temperature incubation step to activate the enzyme. The advanced aptamer-based hot-start activity coupled with the supplied optimized reaction buffer makes the EpiMark Hot Start Taq DNA Polymerase an excellent choice for use on bisulfite-converted DNA. Taq DNA polymerase possesses a 5- 3 polymerase activity (1,2,3) and a 5 flap endonuclease activity (4,5). EpiMark Hot Start Taq DNA Polymerase is supplied with 5X EpiMark Hot Start Taq Reaction Buffer, providing robust amplification for bisulfite-converted DNA and AT-rich amplicons.
Discovery Life Sciences HUMAN UGT1A1 0.5ML@5MG/ML
BD Gentest, Human UGT1A1, Regulatory Status is RUO, 0.5mL@5mg/mL
Apexbio Technology LLC PreScission Protease (PSP), 500U (1U/uL). Cas: MFCD: NA. Specifically recognize and cleave the Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro peptide in fusion proteins.
PreScission Protease is a fusion protein of human rhinovirus 3C protease and glutothione S trasferase (GST). PreScisson protease can specifically recognize the Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro sequence and cleaves between the Gln and Gly residues. Concentration: 2U/L Other sizes are also available. Please inqury us for quote.