Modifying Enzymes
TARGETMOL CHEMICALS INC AMINOPEPTIDASE M 100MG
Also available in 25 mg 50 mg 200 mg and bulk. Please contact Fisher for quotes. Aminopeptidase M (AMP) inhibition is of interest for several diseases such as highly vascularized cancer types. purity: 98%
ABclonal Technology DNA Ligase III/LIG3 Rabbit pAb
This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Biotium Topoisomerase I, MT(TOP1MT/488), CF740 conjugate, 0.1mg/mL
DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can
American Research Products Inc BoVine DeoXyribonuclease 1 DN
BoVine DeoXyribonuclease-1 (DNASE1) ELISA kit from CUSABIO Detection Range 12 5 pg/mL-800 pg/mL Sensitivity 3 1 pg/mL Sample serum plasma tissue homogenates Method Sandwich
INTACT GENOMICS INC Endonuclease IV (Nfo) 2000 Units (10 units/µl)
Endonuclease IV (Nfo) from Escherichia coli is a 32-kD metalloprotein that aids in the repair of damaged DNA. The enzyme functions both as an apurinic/apyrimidinic nuclease (1) and as a 3′-terminal di-esterase (1-4). Its 3′-terminal di-esterase activity is important in the repair of DNA strand breaks generated by oxidation and ionic radiation (2, 3). In such events, the strand breaks terminate with either a 3′ phosphate or a deoxyribose fragment, preventing repair by DNA polymerase I or DNA ligase. Endonuclease IV removes the blocking groups, leaving a free 3′-hydroxyl terminus. This enzyme does not have detectable associated exonuclease or DNA N-glycosylase activity (1).Applications• Single cell gel electrophoresis (Comet assay) (5, 6)• Alkaline elution (7)• Alkaline unwinding (8)Product SourceE. coli BL21 (DE3) strain expressing E. coli Endonuclease IV gene.Product Includes• Endonuclease IV (Nfo)• 10x Endonuclease IV reaction buffer
Medchemexpress LLC DNA Ligase IV antibody (YA2699) | 10 UL
DNA Ligase IV antibody (YA2699) is a rabbit-derived, non-conjugated IgG monoclonal antibody that targets DNA Ligase IV. This antibody plays a critical role in DNA non-homologous end joining (NHEJ), specifically in double-strand break (DSB) repair and V(D)J recombination, by catalyzing the NHEJ ligation step to reseal DNA breaks.
- Rabbit-derived IgG monoclonal antibody
- Non-conjugated
- Targets DNA Ligase IV
- Reactivity: Human
- Application: WB (Western Blot)
- Dilution ratio: 1:500-1:1000 for WB
- SwissProt ID: P49917
- Research field: Epigenetics and nuclear signaling
- Supplied in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
- Appearance: Liquid
- Storage: Stored at -20°C for 1 year, avoid repeated freeze/thaw cycles
Worthington Biochemical Corporation GALACTOSIDASE BETA 5KU
A partially purified, lyophilized powder. 2604-85-1
Apexbio Technology LLC T4 DNA Ligase (RNase-free)(Synonyms: T4 DNA Ligase, RNase-free T4 DNA Ligase, T4 Ligase, T4 DNA Joining Enzyme), 100KU.
T4 DNA Ligase (RNase-free) is an ATP-dependent enzyme with a molecular weight of approximately 55 3 kDa catalyzing the formation of phosphodiester bonds between adjacent 5 -phosphate and 3 -hydroxyl termini in double-stranded DNA and RNA This enzyme ligates DNA fragments possessing either cohesive or blunt ends and repairs single-strand breaks in double-stranded DNA RNA or DNA/RNA hybrids It is extensively utilized in molecular cloning linker or adaptor ligation nick-repair assays and circularization of linear DNA molecules
Cayman Chemical Precelys 24 User MaIntena 1ea
This kit provides Precellys 24 users with all of the necessary parts to properly maintain their instrument. EAch kit contains: 1 x Precellys 24 White Blocking Plate (2 mL) (Item No. 10422) 2 x Precellys 24 Vacuum Joint (toric joint) (Item No. 10011148) 1 x Precellys 24 Containment Seal (Item No. 10011149)] 1 x Precellys 24 Anti-Rotation Tubing Kit - (Item No. 10011162) [Bertin Catalog S000688-PR240-A.0; Previously SP03119-810-NC011.0]
ABclonal Technology DNase I , RNase-free (5,000 U/mL)
DNase I （deoxyribonuclease, RNase-free ） is an endonuclease that nonspecifically cleaves DNA to release di-, tri-, and oligonucleotide products with 5phosphorylated and 3?-hydroxylated ends. The activity of DNase I depends on Ca2+ and also be activated bydivalent metal ions Mg2+, Mn2+, etc.DNase I act on various DNAs such as single and double-stranded DNA, RNA:DNA hybrids.
Medchemexpress LLC Topoisomerase II inhibitor 14 | 305343-00-4 | MFCD06592467 | 99.7% | 332.19 g/mol | C15H11Cl2N5 | 5 MG
Topoisomerase II inhibitor 14 is a research-grade small molecule inhibitor of topoisomerase II. It is described as a potent inhibitor that induces apoptosis, arrests the cell cycle at S and G2-M phases, and exhibits antioxidant activity. The compound is provided as a high-purity solid and is intended for laboratory research use only.
- Potent topoisomerase II inhibition with cellular activity.
- Induces apoptosis and cell cycle arrest for mechanistic studies.
- Exhibits antioxidant effects useful in oxidative stress research.
- High purity appropriate for biochemical and cellular assays.
- Available in small packs suitable for screening and pilot studies.
- Soluble in dimethyl sulfoxide (DMSO) with recommended warming and sonication.
- Storage conditions specified for solid and solution to preserve stability.
Carbosynth LLC. 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt monohydrate, 114162-64-0, MFCD00058543, 1 g
Formula: C20H28BrClN2O8, Molecular weight: 539.81 g/mol, 99%, Store at -15°C, Synonyms: X-beta-D-glucuronide cyclohexylammonium salt; X-beta-D-glucuronide CHX-salt; X-beta-D-GlcA · CHX.
Enzo Life Sciences USP2 (catalytic domain) (rat), (recombinant) (untagged) (100 µg)
Alternative name: Ubiquitin specific protease 2. MW: ~40kDa. Purity: ≥95% (SDS-PAGE, following ion exchange chromatography). Formulation: Liquid. In 10mM MES, 0.5mM DTT, 50mM TRIS-HCl, pH 7.5, containing 20% glycerol. Source: Produced in E. coli BL21 (λDE3). Untagged USP2 catalytic domain. UniProt: Q5U349. Handling: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. Long Term Storage: -80°C
Apexbio Technology LLC Amyloid β-Peptide (1-42) (human),1mg CAS# 107761-42-2
Amyloid β-Peptide (1-42) (Aβ42) human is a 42-amino acid peptide that plays a key role in the pathogenesis of Alzheimer disease (AD) [1].The main deleterious effects in the pathogenesis are probably regulated by Aβ42, which acts as a repressor or activator of gene transcription causing further synaptic function damage and neuronal degeneration [2].Aβ42 reduced the viability of SH-SY5Y cells to 65% when it rose to 2.5 μM. Using the chromatin immunoprecipitation (ChIP) and qRT-PCR assays, treatment of SH-SY5Y cells with Aβ42 associated peptide with both LRP1 and KAI1 promoters and increased APP mRNA levels, while the nontoxic Aβ42 G33A peptide, as a control group which presents in the nucleus abundantly, did not have any influence on mRNA expression. The exclusive increase of transcription and expression of its precursor gene APP was found with the treatment of Aβ42, compared with several different lengths of peptides [1]. Other sizes are also available. Please inqury us for quote.
Cayman Chemical N-Oleoyl GlutamIn 10mg
An endogenous N-acyl amine; induces mitochondrial uncoupling to increase respiration in C2C12 cells by 64% at 50 µM; inhibits capsaicin-induced calcium flux in HEK293A cells co-transfected with TRPV1 and RFP; reduces the number of acetic acid-induced abdominal constrictions, as well as formalin-induced paw licking in mice at 100 mg/kg