Modifying Enzymes
Amsbio LLC Heparinase II from Flavobacterium heparinum/ 0.2 IU
Heparinase II from Flavobacterium heparinum 0.2 IU
LifeSensors SUMOSTAR PROTEASE 1000 UNITS
Recombinant SUMOstar Protease 1 specifically recognizes and cleaves the SUMOstar tag from recombinant fusion proteins made with SUMOstar expression systems (Cat.#s: 1100A, 1106, 1100K, 1101, 2100, 2101, 2160, 2161, 3100, 3101, 7110, 7111, 7120, 7121). Unlike thrombin, EK, or TEV proteases, which recognize short, linear sequences, SUMOstar Protease 1 recognizes the tertiary structure of SUMOstar. As a result, SUMOstar Protease 1 will not cleave within the protein of interest. SUMOstar Protease 1 will not cleave SUMO-tags from proteins expressed with the SUMOpro or SUMOpro3 systems. To cleave the SUMOpro tag (orginial yeast SUMO tag), please use SUMO Protease 1 and to cleave SUMOpro3 tag please use SUMO Protease 2. This product contains 1000 units of SUMOstar Protease, where 1 U will cleave >90 µg of SUMOstar-GFP in 1 hour at 37C
Aldevron Guanylyltransferase, 1750ug
1750ug dispense of Guanylyltransferase. Guanyltransferase (GT), also known as Vaccinia Capping Enzyme (VCE), catalyzes addition of 7-methylguanylate cap structures (Cap 0) to the 5’ end of RNA in three steps:
Reactions 1 & 2: Conversion of an RNA substrate pppN(pN)n to GpppN(pN)n with increasing amounts of enzyme was visualized by gel electrophoresis (uncapped, lower band; capped, upper band). One unit (U) is the amount of enzyme activity that caps 50% of a 5’-ppp oligo in 30 minutes at 37°C.
Reaction 3: Methyl transfer from SAM to GpppN(pN)n producing SAH and cap-0 mRNA was measured using the EPIgeneousTM Methyltransferase Assay (Cisbio). One unit (U) is the amount of enzyme activity that produces 1 pmol of SAH from SAM in 30 minutes at 37°C using cap-0 substrate.
Echelon Biosciences Research Labs GLO-PI(3,4,5)P3 BODIPYTMR50ug
BODIPY TMR PI(3,4,5)P3 (BODIPY TMR Phosphatidylinositol 3,4,5-trisphosphate) is a water soluble analog of PI(3,4,5)P3 (PIP3) labeled with the orange fluorophore, Bodipy-TMR, at the sn-1 position.Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 3,4,5-trisphosphate (PIP3), formed from PI(4,5)P2 though phosphorylation by PI 3-kinase, activates numerous signaling pathways resulting in cell proliferation, growth, survival, glucose transport and protein synthesis. High PIP3 levels from disregulation of PI3-K have been demonstrated in cancer and inflammatory diseases. PIP3 is hydrolyzed by the phosphatases PTEN to PI(4,5)P2 and SHIP to PI(3,4)P2. This item is non returnable
Carnabio Usa Inc AMPKA1/B1/G1(PRKAA1/B1/G1)/10U
AMPK1/1/1(PRKAA1/B1/G1), Full-length, Wild type, Amino Acid 1-550, NP_006242.4, Expressed in Insect (sf21); 10microg
Carnabio Usa Inc WNK3/10UG/CATALYTIC
WNK3, Catalytic domain, Wild type, Amino Acid 1-434, NP_065973.2, Expressed in Insect (sf21); 10microg
Carnabio Usa Inc PKCB1(PRKCB1)/5UG/FULL-LENGTH
PKC1(PRKCB1), Full-length, Wild type, Amino Acid 1-671, NP_997700.1, Expressed in Insect (sf21); 5microg
Carnabio Usa Inc BTN - ALK / 10UG
BTN-ALK, Cytoplasmic domain, Wild type, Amino Acid 1058-1620(end), BAG10812.1, Expressed in Insect (sf21); 10microg
Carnabio Usa Inc RSK1(RPS6KA1)/5UG/FULL-LENGTH
RSK1(RPS6KA1), Full-length, Wild type, Amino Acid 1-735, NP_002944.2, Expressed in Insect (sf21); 5microg
Carnabio Usa Inc IKKB(IKBKB)/10UG/TRUNCATED
IKK(IKBKB), Truncated protein, Wild type, Amino Acid 1-662 , NP_001547.1, Expressed in Insect (sf21); 10microg
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Amsbio LLC Neuraminidase [Sialidase]; Acylneuraminyl hydrolase [EC 3.2.1.18]
Neuraminidase [Sialidase]; Acylneuraminyl hydrolase [EC 3.2.1.18]