Modifying Enzymes
New England Biolabs, Inc. PNGase A – 150 units
PNGase A cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and short complex oligosaccharides such as those found in plant and insect cells from N-linked glycoproteins and glycopeptides. PNGase A differs from PNGase F in that it cleaves N-linked glycans with or without (1,3)-linked core fucose residues.
New England Biolabs, Inc. Bpu10I – 200 units
A restriction endonuclease that recognizes the sequence CC^TNA_GC.
Sigma Aldrich Fine Chemicals Biosciences beta-Amylase from sweet potato | 9000-91-3 | MFCD00081391 | 1VL
beta-Amylase from sweet potato | 9000-91-3 | MFCD00081391 | 1VL
LGC GENOMICS LLC (Lucigen) Plasmid-Safe ATP-Dependent DNase, 10,000 U @ 10 U/LDigest unwanted contaminating linear double-stranded DNA, without harming circular double-stranded DNA such as plasmids and fosmids.
Plasmid-Safe™ ATP-Dependent DNase selectively removes contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC clones or vector preparations.
Sigma Aldrich Fine Chemicals Biosciences Catalase from bovine liver lyophilized powder, 2,000-5,000 units/mg protein | 9001-05-2 | MFCD00081483 | 1G
Catalase from bovine liver lyophilized powder, 2,000-5,000 units/mg protein | 9001-05-2 | MFCD00081483 | 1G
Enzo Life Sciences Granzyme B (human), (recombinant) (5000 U)
MW: 25.4 kDa. Concentration: 100 U/µl. Formulation: Liquid. In 50mM TRIS, pH 7.4, 100mM NaCl, 10% glycerol. Source: Produced in yeast. Recombinant human granzyme B (aa 21-247). UniProt: P10144. Long Term Storage: -80°C
LGC GENOMICS LLC (Lucigen) Ampligase DNA Ligase with Buffer, 2,500 U @ 5 U/LHighly thermostable DNA ligase for applications where ligation at high temperature is beneficial or required.
Ampligase Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3-hydroxylated and 5-phosphorylated termini in duplex DNA structures that are stable at high temperatures
Sigma Aldrich Fine Chemicals Biosciences Nuclease P1 from Penicillium citrinum, 54576-84-0, MFCD00131010, 1VL
Lyophilized powder, ≥200 units/mg protein (E1%/280, 3′-5′-Phosphodiesterase), Synonym: Endonuclease P1. Nuclease P1 from Penicillium citrinum has been used in a study to assess crystal structures using ammonium sulphate or polyethylene glycol 4000 as a precipitating agent. It has also been used in a study to investigate a method for the direct sequence analysis 20-25 nucleotides from the terinini of 5′ or 3′ end group labeled RNA. Nuclease P1 is used to improve the sensitivity of a 32P-labeling method for the detection of DNA adducts.
Sigma Aldrich Fine Chemicals Biosciences Alcohol Dehydrogenase from Saccharomyces cerevisiae | 9031-72-5 | MFCD00081305 | 1VL
Alcohol Dehydrogenase from Saccharomyces cerevisiae | 9031-72-5 | MFCD00081305 | 1VL
LGC GENOMICS LLC (Lucigen) Baseline-ZERO DNase, 5000 UDigest large or small DNA molecules better than standard DNase I.
Baseline-ZERO™ DNase digests dsDNA and ssDNA into mononucleotides more effectively than the commonly used bovine pancreatic DNase I.
Sigma Aldrich Fine Chemicals Biosciences Protease from Aspergillus oryzae | 1395-21-7 | MFCD00132092 | 250mL
Protease from Aspergillus oryzae | 1395-21-7 | MFCD00132092 | 250mL
LGC GENOMICS LLC (Lucigen) Terminator 5'-Phosphate-Dependent Exonuclease, 40U @ 1 U/LA processive 5 to 3 riboexonuclease that specifically digests RNA with 5-monophosphate ends only.
Terminator™ 5-Phosphate-Dependent Exonuclease is a processive 53 exonuclease that digests RNA that has a 5-monophosphate end. It does not digest RNA that has a 5-triphosphate, 5-cap or 5-hydroxyl group.
LGC GENOMICS LLC (Lucigen) Ampligase DNA Ligase without Buffer, 10,000 U @ 100 U/LHighly thermostable DNA ligase for applications where ligation at high temperature is beneficial or required.
Ampligase Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3-hydroxylated and 5-phosphorylated termini in duplex DNA structures that are stable at high temperaturesHigh thermostability allows ligation using high-stringency hybridization conditionsHigh specificity and stringency permits sensitive detection of SNPsAvailable in kit and "enzyme only" formats11