Modifying Enzymes
Sigma Aldrich Fine Chemicals Biosciences DNase I, 100 mg
During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, therby avoiding the loss of cells from undesired clumping.
Accelagen TurboNuclease 250,000U, Endonuclease, 250 units/μl, 27 kDa, >99% purity, -20°C storage, 10-500 units/g cell paste, 25 units/mL in cell lysate, 5'-phosphorylated oligonucleotides 1-4 bases
TurboNuclease 250,000U, Endonuclease, 250 units/μl, 27 kDa, >99% purity, -20°C storage, 10-500 units/g cell paste, 25 units/mL in cell lysate, 5'-phosphorylated oligonucleotides 1-4 bases
Genscript Corporation 3C protease, His, Human
Recombinant PreScission Protease (rPSP) from human rhinovirus (HRV 3C) is a recombinant restriction-grade protease. PSP is a highly purified recombinant 6xHis-fusion protein, which recognizes the same cleavage site as the native enzyme. It is used to cleave affinity tags from fusion proteins. The optimum recognition site for this enzyme is the sequence Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro(LEVLFQ/GP) and cleavage occurs between the Gln and Gly-Pro residues. The optimal temperature for cleavage is 4°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant PreScission Protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.
Worthington Biochemical Corporation PAPAIN DISSOCIATION SYSTEM
Papain Dissociation System, 100mL of EBBS, 2degree to 8degreeC Storage Temp, 1BX
Sigma Aldrich Fine Chemicals Biosciences Lysozyme from chicken egg white, 12650-88-3, MFCD00131557, 1 g
Lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein, Synonym: Mucopeptide N-acetylmuramoylhydrolase, Muramidase. Lysozyme from chicken egg white has been used for the extraction of genomic DNA from bacterial cells. It has been used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis.
LGC GENOMICS LLC (Lucigen) Ready-Lyse Lysozyme Solution, 4,000,000 UReady-to-use lysozyme solution to be used with our nucleic acid purification kits.
Ready-Lyse™ Lysozyme Solution is a recombinant lysozyme preparation, derived from a nonmammalian, nonavian source, that is useful for the lysis of Gram-negative (such as E. coli) and Gram-positive (such as Bacillus sp.) bacteria.
New England Biolabs, Inc. T5 Exonuclease – 1000 units
T5 Exonuclease degrades DNA in the 5 to 3 direction. T5 Exonuclease is able to initiate nucleotide removal from the 5 termini or at gaps and nicks of linear or circular dsDNA. However, the enzyme does not degrade supercoiled dsDNA. T5 Exonuclease also has ssDNA endonuclease activity.
Worthington Biochemical Corporation PDS KIT 5/PK
Papain Dissociation System, Papain Vial, Code: PAP2, Vial reconstituted with 5mL of EBSS or equivalent yields a solution at 20U of papain permL in 1mm L-cysteine with 0.5mm EDTA, Store at 2 deg. to 8 deg.C, 5Vi
Gold Biotechnology Inc DNase I, Bovine Pancreas (Lysate Tested), >2000 Kunitz U/mg 100 mg
Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease, which splits phosphodiester linkages preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and a phosphate group at the 5' position. The optimum pH is 7.8.
GENOVIS INC Glyserias, 2000 units
Glyserias is an enzyme that cleaves the Fc portion of IgG antibodies. It is produced by Genovis and sold in vials containing 2000 units of the enzyme. Glyserias works optimally at pH 5.5-6.5 and 37°C. It can cleave both native and recombinant IgGs of all subclasses in just 30 minutes. Glyserias enables antibody deglycosylation, antibody fragmentation, and Fc/Fab separation. It has applications in structural biology, effector function studies, mass spec analysis, and antibody drug development. Glyserias provides an easy and gentle method of IgG cleavage compared to traditional proteolytic enzymes.
New England Biolabs, Inc. Thermolabile Exonuclease I – 15000 units
Thermolabile Exonuclease I catalyzes the removal of nucleotides from single-stranded DNA (ssDNA) in the 3' to 5' direction.
SCIENCELL RESEARCH LABORATORIES INC TRYPSIN NEUTRALIZATION SOL 200
NC2331260 TRYPSIN NEUTRALIZATION SOL 200
Worthington Biochemical Corporation Trypsin, TPCK Treated, 100mg
A chromatographically purified, diafiltered, lyophilized powder that has been treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity [Kostka, V., and Carpenter, F.: JBC, 239, 1799 (1964)]. Store at 2-8°C. Protect from moisture.