Modifying Enzymes
New England Biolabs, Inc. Rapid™ PNGase F – 50 reactions
Rapid PNGase F enables complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins, in minutes. All N-glycans are released rapidly and without bias, and are ready to be prepared for downstream chromatography or mass spectrometry analysis. Rapid PNGase F creates an optimized workflow, reducing processing time without compromising sensitivity or reproducibility.
- Complete deglycosylation of antibodies and immunoglobulin fusion proteins in 10 minutes
- Recombinant enzyme; guaranteed endoglycosidase F1, F2 or F3 free
- All reaction components are compatible with HPLC and mass spectrometry analysis
- >= 95% purity, as determined by SDS-PAGE and intact ESI-MS
- Optimal activity and stability ensured for up to 12 months
Sigma Aldrich Fine Chemicals Biosciences Liberase TL Research Grade, 10 mg
Liberase TL (Thermolysin Low) Research Grade is used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high cell yield and viability. Bacterial by-products, such as endotoxins, are reduced up to several thousand-fold.
Worthington Biochemical Corporation HYALURONIDASE 300KU
Hyaluronidase, 300 ku/Pack, A partially purified, dialyzed, lyophilized powder, Store at -20degreeC, Source: Bovine Testes, Minimum Activity: =300 USP/NF units per mg dry weight
New England Biolabs, Inc. T3 DNA Ligase – 100000 units
T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3. It will catalyze the formation of a phosphodiester bond between adjacent 5 phosphate and 3 hydroxyl groups of duplex DNA. Cohesive ends, blunt ends, and nick sealing can all be efficiently catalyzed by T3 DNA Ligase. As with T4 DNA Ligase, blunt end ligation is enhanced by the addition of PEG 6000 to the reaction. T3 DNA Ligase exhibits a higher tolerance (2-fold) for NaCl in the reaction compared to T4 DNA Ligase, making the enzyme a versatile choice for in vitro molecular biology protocols requiring DNA ligase activity.
New England Biolabs, Inc. T5 Exonuclease – 1000 units
T5 Exonuclease degrades DNA in the 5 to 3 direction. T5 Exonuclease is able to initiate nucleotide removal from the 5 termini or at gaps and nicks of linear or circular dsDNA. However, the enzyme does not degrade supercoiled dsDNA. T5 Exonuclease also has ssDNA endonuclease activity.
Worthington Biochemical Corporation PDS KIT 5/PK
Papain Dissociation System, Papain Vial, Code: PAP2, Vial reconstituted with 5mL of EBSS or equivalent yields a solution at 20U of papain permL in 1mm L-cysteine with 0.5mm EDTA, Store at 2 deg. to 8 deg.C, 5Vi
New England Biolabs, Inc. Thermolabile Exonuclease I – 3000 units
Thermolabile Exonuclease I catalyzes the removal of nucleotides from single-stranded DNA (ssDNA) in the 3' to 5' direction.
LGC GENOMICS LLC (Lucigen) Ready-Lyse Lysozyme Solution, 4,000,000 UReady-to-use lysozyme solution to be used with our nucleic acid purification kits.
Ready-Lyse™ Lysozyme Solution is a recombinant lysozyme preparation, derived from a nonmammalian, nonavian source, that is useful for the lysis of Gram-negative (such as E. coli) and Gram-positive (such as Bacillus sp.) bacteria.
Sigma Aldrich Fine Chemicals Biosciences DNase I, 100 mg
During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, therby avoiding the loss of cells from undesired clumping.
SCIENCELL RESEARCH LABORATORIES INC TRYPSIN NEUTRALIZATION SOL 200
NC2331260 TRYPSIN NEUTRALIZATION SOL 200
Sigma Aldrich Fine Chemicals Biosciences Pepsin from Porcine Gastric Mucosa | 9001-75-6 | MFCD00081840 | 1g
Pepsin from Porcine Gastric Mucosa | 9001-75-6 | MFCD00081840 | 1g
New England Biolabs, Inc. T7 DNA Ligase – 100000 units
T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7. It will catalyze the formation of a phosphodiester bond between adjacent 5' phosphate and 3' hydroxyl groups of duplex DNA. Cohesive end ligation and nick sealing can be efficiently catalyzed by T7 DNA Ligase. However, unlike T4 and T3 DNA Ligases, blunt end ligation is not efficiently catalyzed by T7 DNA Ligase. Addition of high concentrations of PEG 6000 [>= 20% (w/v)] to the reaction can force T7 DNA Ligase to have measurable activity. However, under typical reaction conditions blunt-end DNA ligation does not occur in the presence of T7 DNA Ligase, making it a good choice for applications in which blunt and cohesive ends of DNA are present but only the cohesive ends are to be joined. T7 DNA Ligase does not ligate blunt-end DNA fragments.