Modifying Enzymes
Gold Biotechnology Inc DNase I, Bovine Pancreas (Lysate Tested), >2000 Kunitz U/mg 100 mg
Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease, which splits phosphodiester linkages preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and a phosphate group at the 5' position. The optimum pH is 7.8.
New England Biolabs, Inc. T4 RNA Ligase 2, truncated K227Q – 2000 units
T4 RNA Ligase 2, truncated K227Q (T4 Rnl2tr K227Q) specifically ligates the pre-adenylated 5' end of DNA or RNA to the 3' OH end of RNA. The enzyme does not use ATP for ligation but requires pre-adenylated linkers. T4 Rnl2tr K227Q is a point mutant of T4 RNA Ligase 2, truncated (NEB# M0242) . Mutation of K227 in T4 RNA Ligase 2 reduces enzyme lysyl adenylation. This mutation further reduces the formation of undesired ligation products (concatemers and circles) by T4 Rnl2tr, possibly by reducing the trace activity of T4 Rnl2tr in transfer of adenylyl groups from linkers to the 5-phosphates of input RNAs. The exclusion of ATP, use of pre-adenylated linkers, and the reduced enzyme lysyl adenylation activity provide the lowest possible background in ligation reactions. This enzyme has been used for optimized linker ligation for the cloning of microRNAs.
Worthington Biochemical Corporation DISPASE 50MG
Neutral Protease (Dispase), Its mild proteolytic action makes the enzyme especially suitable for the preparation of primary and secondary (subcultivation) cell culture, A lyophilized powder, Pack Size: 5mg
Agilent Technologies 100MU/40UL GLYKO N-GLYCANASE
PNGase F, peptide-N-glycosidase F,peptide-N4-(N-acetyl--glucosaminyl)asparagine amidase.Releases intact N-glycans by cleavingbetween the innermost GlcNAc and Asn.
Worthington Biochemical Corporation DEOXYRIBONUCLEASE I, RECOMBINA
Recombinant Bovine pancreatic Deoxyribonuclease 1 produced in Pichia pastoris. Chromatographically purified. Free of animal derived components, RNases, and proteases. A lyophilized powder containing glycine as a stabilizer.
New England Biolabs, Inc. T5 Exonuclease – 1000 units
T5 Exonuclease degrades DNA in the 5 to 3 direction. T5 Exonuclease is able to initiate nucleotide removal from the 5 termini or at gaps and nicks of linear or circular dsDNA. However, the enzyme does not degrade supercoiled dsDNA. T5 Exonuclease also has ssDNA endonuclease activity.
Discovery Life Sciences HUMAN CYP2J2 + REDUCTASE + B5
BD Gentest, Human CYP2J2 + Reductase + B5, Regulatory Status is RUO
Discovery Life Sciences HUMAN CYP19 + P450 REDUCTASE S
Human CYP19 (Aromatase) + Reductase, BD Gentest;
Lifeline Cell Technology Trypsin 0.05% EDTA 0.02% (phenol red free), 100mL
Trypsin acts as an enzyme to help dissociate cells from their culture vessel. EDTA is a calcium chelator that helps to prevent clumping of cells. 0.05% Trypsin/0.02% EDTA Solution may be stored at -20°C until the expiration date or at 4-8°C for up to 1 month. This product is not approved for human or veterinary use or for use in in vitro diagnostics or clinical procedures.
Worthington Biochemical Corporation DIAPHORASE 2KU
A lyophilized powder. Dialyzed to remove pyridine nucleotides.
Sigma Aldrich Fine Chemicals Biosciences Catalase from bovine liver | 9001-05-2 | MFCD00081483 | 500mg
Catalase from bovine liver | Mol Wt: tetramer -250 kDa | 9001-05-2 | MFCD00081483 | 500mg
Genscript Corporation 3C protease, His, Human
Recombinant PreScission Protease (rPSP) from human rhinovirus (HRV 3C) is a recombinant restriction-grade protease. PSP is a highly purified recombinant 6xHis-fusion protein, which recognizes the same cleavage site as the native enzyme. It is used to cleave affinity tags from fusion proteins. The optimum recognition site for this enzyme is the sequence Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro(LEVLFQ/GP) and cleavage occurs between the Gln and Gly-Pro residues. The optimal temperature for cleavage is 4°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant PreScission Protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.