Modifying Enzymes
Research Products International Corp Proteinase K, 100 Milligrams
From Tritirachium album. Serine protease for proteolytic inactivation of nucleases during isolation of DNA and RNA.
Description
Proteinase K is a highly reactive serine protease that displays an ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It is the most powerful proteinase among all proteinases characterized so far. It cleaves at the peptide bond adjacent to the carboxylic acid group of aliphatic, aromatic or hydrophobic amino acids.
Recombinant Proteinase K is used in the isolation or preparation of high molecular weight nucleic acids. It is highly pure and has a higher specific activity and is more stable at room temperature when compared to native Proteinase K.
![Amsbio LLC Neuraminidase isozyme S [Reagent for studies of gangliosides]](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502566244.png-250.jpg)
Amsbio LLC Neuraminidase isozyme S [Reagent for studies of gangliosides]
Neuraminidase isozyme S [Reagent for studies of gangliosides]
Creative Biomart Native Bacillus licheniformis Protease
AbbrProtease, Native (Bacillus licheniformis)AliasProteaseSourceBacillus licheniformisApplicationsThe product has been used with other enzymes for in situ proteolysis to produce crystals suitable for structure determination. It has also been used in the process of isolation of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria that can be used for functional in vitro studies. This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions.Product OverviewThe enzyme is found to be stable at pH 8-10, retaining activity of up to 90% for 24 hours. It shows maximum activity at temperatures between 55-60°C.FormType VIII, lyophilized powder; Type I, aqueous solutionEnzyme Commission NumberEC 3.4.21.62
ABclonal Technology T4 RNA Ligase 2, truncated KQ
T4 RNA Ligase 2, truncated KQ (T4 Rnl2tr R55K, K227Q), specifically catalyzes the ligation of a 5-adenylated DNA or RNA to the 3 OH end of an RNA molecule. This enzyme-mediated ligation does not require ATP but necessitates a pre-adenylated adapter. T4 RNA Ligase 2, truncated KQ, a double-point mutant of T4 RNA Ligase 2 with R55K and K227Q substitutions, significantly reduces the non-specific ligation of RNA (such as concatenation or self-circularization) compared to T4 RNA Ligase 2, truncated, while maintaining enzyme activity similar to that of T4 RNA Ligase 3, truncated.
Sigma Aldrich Fine Chemicals Biosciences Elastase from human leukocytes | 9004-06-2 | MFCD00130997 | 1un
Elastase from human leukocytes | Mol Wt: 29 kDa | 9004-06-2 | MFCD00130997 | 1un
Medchemexpress LLC α-amylase-IN-3 | 93944-58-2 | 99.5% | 267.24 g/mol | C15H9NO4 | 10 MG
α-Amylase-IN-3 is a small-molecule research reagent reported to inhibit α-amylase and acetylcholinesterase, and to exhibit antioxidant activity. It is supplied as a solid for laboratory research and is used in studies related to diabetes and oxidative stress-associated disease.
- Non-competitive α-amylase inhibitor with reported IC50 ≈ 18.04 μM.
- Acetylcholinesterase inhibitory activity with reported IC50s ≈ 21.04 μM and 22.2 μM.
- Demonstrates antioxidant activity in standard radical-scavenging assays.
- Supplied as a stable solid suitable for laboratory storage and handling.
- High reported purity (≈99.49% by HPLC).
- Molecular weight 267.24 g/mol; chemical formula C15H9NO4.
Creative Biomart Native Bacillus licheniformis Protease
AbbrProtease, Native (Bacillus licheniformis)AliasProteaseSourceBacillus licheniformisApplicationsThe product has been used with other enzymes for in situ proteolysis to produce crystals suitable for structure determination. It has also been used in the process of isolation of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria that can be used for functional in vitro studies. This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions.Product OverviewThe enzyme is found to be stable at pH 8-10, retaining activity of up to 90% for 24 hours. It shows maximum activity at temperatures between 55-60°C.FormType VIII, lyophilized powder; Type I, aqueous solutionEnzyme Commission NumberEC 3.4.21.62
Aldevron SpyFi Cas9 Nuclease, 1mg
5mg dispense size of SpyFi Cas9 Nuclease. From basic research to clinical applications, our SpyFi Cas9 Nuclease delivers greater editing efficiency and reduced off-target effects without loss of on-target activity to help accelerate your CRISPR-Cas9 gene editing program. SpyFi Cas9 Nucleases are available at Research-Grade through Fisher, or across all quality grades (Research Grade or GMP) directly through Aldevron.
SpyFi Cas9 Nuclease is uniquely positioned to satisfy a wide range of needs from basic research to clinical applications. When used in combination with effective guide, target site and double-stranded DNA cut strategies, SpyFi Cas9 Nuclease delivers greater editing efficiency, higher fidelity and less off-target activity than wild-type SpCas9 nuclease.
Functional Performance
•Reduced off-target effects
•Ready to use for electroporation or transfection
•10 mg/mL formulation for ease of use
Carnabio Usa Inc BTN - MET / 10UG
BTN-MET, Cytoplasmic domain, Wild type, Amino Acid 956-1390(end), NP_000236.2, Expressed in Insect (sf21); 10microg
Sigma Aldrich Fine Chemicals Biosciences Amano Lipase A from Aspergillus niger >=120,000 U/g | 9001-62-1 | MFCD02685889 | 10G
Amano Lipase A from Aspergillus niger >=120,000 U/g | 9001-62-1 | MFCD02685889 | 10G
Sigma Aldrich Fine Chemicals Biosciences Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae) | 9001-40-5 | MFCD00081656 | 1ku
Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae) | Mol Wt: 128 kDa | 9001-40-5 | MFCD00081656 | 1ku
Cayman Chemical EthylmaLNyl Coenzyme AsodI
An intermediate in the ethylmalonyl-CoA pathway in bacteria
Sigma Aldrich Fine Chemicals Biosciences Lipase acrylic resin >=5,000 U/g, recombinant, expressed in Aspergillus niger | 9001-62-1 | MFCD00217536 | 3G
Lipase acrylic resin >=5,000 U/g, recombinant, expressed in Aspergillus niger | 9001-62-1 | MFCD00217536 | 3G
Cayman Chemical Oleoyl-D-lysInsodIum salt 25mg
An inhibitor of GlyT2a; selectively inhibits GlyT2a (IC50 = 48.3 nM) over GlyT1b (IC50 = >3,000 nM) in Xenopus oocytes expressing the human transporters; reduces allodynia in a nerve ligation rat model of neuropathic pain at 30 mg/kg, i.p.