Modifying Enzymes
New England Biolabs, Inc. RNase HII – 250 units
Ribonuclease HII (RNase HII) is an endoribonuclease that preferentially nicks 5' to a ribonucleotide within the context of a DNA duplex. The enzyme leaves 5' phosphate and 3' hydroxyl ends. RNase HII will also nick at multiple sites along the RNA portion of an Okazaki fragment.
Worthington Biochemical Corporation HYALURONIDASE 300KU
Hyaluronidase, 300 ku/Pack, A partially purified, dialyzed, lyophilized powder, Store at -20degreeC, Source: Bovine Testes, Minimum Activity: =300 USP/NF units per mg dry weight
Research Products International Corp Proteinase K, 100 Milligrams
From Tritirachium album. Serine protease for proteolytic inactivation of nucleases during isolation of DNA and RNA.
Description
Proteinase K is a highly reactive serine protease that displays an ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It is the most powerful proteinase among all proteinases characterized so far. It cleaves at the peptide bond adjacent to the carboxylic acid group of aliphatic, aromatic or hydrophobic amino acids.
Recombinant Proteinase K is used in the isolation or preparation of high molecular weight nucleic acids. It is highly pure and has a higher specific activity and is more stable at room temperature when compared to native Proteinase K.
Takara Bio RECOMBINA DNASE I (RNASE-FREE)
Enzyme, Modifying; Recombinant DNase I (RNase-free); Takara; 5000U; 5U/uL; Source: Expressed in non-animal host carrying plasmid encodes the gene of bovine pancreas DNase I; Storage: -20 deg.C; Buffers included
MOLECULAR DEPOT LLC Xanthine Oxidase - 100 U
Xanthine Oxidase Grade II (Lyophilized Powder) consists of a highly purified native Xanthine oxidase supplied as a lyophilized form Xanthine oxidase uses molybdenum as cofactor and plays a central role in the metabolism of nucleotides It is extensively used in enzymatic assays for the determination of inorganic phosphorus 5-nucleotidase (5-NT) and adenosine deaminase (ADA) For in vitro research use only
Cell Biolabs Inc OxiSelect™ Myeloperoxidase Chlorination Activity Assay Kit (Colorimetric), 200 assays
Myeloperoxidase (MPO) is a peroxidase enzyme highly expressed in stimuated neutrophil granulocytes. The enzyme has two activities: chlorination and peroxidation. Its chlorination activity produces hypochlorous acid (HOCl), a reactive oxygen intermediate that helps the neutrophils kill microbes. Cell Biolabs' OxiSelect™ Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, colorimetric assay for measuring the chlorination activity of myeloperoxidase within a sample. Each kit provides sufficient reagents for 200 assays including standards and unknown samples.
American Research Products Inc AMERICAN RESEARCH PRODUCTS INC
5000615743 TOPOISOMERASE III ELISA KIT
INTACT GENOMICS INC T4 DNA Ligase 400,000 Units (2000 units/µl)
Intact Genomics T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. This enzyme joins DNA fragments with either cohesive or blunt termini and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids (1).Applications• Cloning of restriction enzyme generated DNA fragments• Cloning of PCR products• Next-gen library preparation• Joining linkers and adapters to cohesive or blunt-ended DNA• Nick repair in duplex DNA, RNA or DNA/RNA hybrids• Self-circularization of linear DNA
New England Biolabs, Inc. CviKI-1 – 250 units
A restriction endonuclease that recognizes the sequence RG^_CY. CviKI-1 is a restriction endonuclease with 4 expected recognition sites as well as up to eleven relaxed non-cognate sites (star sites).
New England Biolabs, Inc. EnGen Cas9 NLS, S. pyogenes – 2000 pmol
EnGen Spy Cas9 NLS, is an RNA-guided endonuclease that catalyzes site- specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif) ). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence. EnGen Spy Cas9 NLS contains Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein.
New England Biolabs, Inc. XhoI – 25000 units
A restriction endonuclease that recognizes the sequence C^TCGA_G.
LGC GENOMICS LLC (Lucigen) NxGen T4 DNA Ligase (Low Concentration)A workhorse, high quality DNA ligase for joining blunt or cohesive ends of dsDNA or dsRNA.
NxGen T4 DNA Ligase (Low Concentration)A workhorse, high quality DNA ligase for joining blunt or cohesive ends of dsDNA or dsRNA.Reliably join double-stranded nucleic acid chains.The low concentration (LC) NxGen T4 DNA Ligase Kit includes T4 DNA Ligase at 2 U/¿L and 10X T4 DNA ligase Buffer. The high concentration(HC) NxGen T4 DNA Ligase Kit includes NxGen T4 DNA Ligase at 10 U/¿L and both 2X Rapid Ligation Buffer and 10X T4 DNA ligase Buffer.
Research Products International Corp Proteinase K, 500 Milligrams
From Tritirachium album. Serine protease for proteolytic inactivation of nucleases during isolation of DNA and RNA.
Description
Proteinase K is a highly reactive serine protease that displays an ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It is the most powerful proteinase among all proteinases characterized so far. It cleaves at the peptide bond adjacent to the carboxylic acid group of aliphatic, aromatic or hydrophobic amino acids.
Recombinant Proteinase K is used in the isolation or preparation of high molecular weight nucleic acids. It is highly pure and has a higher specific activity and is more stable at room temperature when compared to native Proteinase K.