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Rat Coagulation Factor X ELISA Kit - Rat Coagulation Factor X ELISA Kit from Innovative Research is intended for the quantitative determination of Rat Coagulation Factor X in biofluid samples, such as plasma. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . F10 present in samples reacts with an anti-Rat F10 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-F10 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active F10 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Rat F10. For RUO
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F7/Coagulation factor VII Recombinant Antibody (clone 3A4) is a recombinant Mouse IgG2a, kappa antibody specific for F7/Coagulation factor VII. It reacts with Human. Suitable for ELISA, WB. Recombinant production ensures high specificity and lot-to-lot reproducibility. Supplied as 100 ug.
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This gene encodes coagulation factor III a cell surface glycoprotein that initiates the blood coagulation cascade by acting as a high-affinity receptor for coagulation factor VII Their complex triggers a catalytic event that activates protease cascades through limited proteolysis Unlike other coagulation cofactors which circulate as inactive precursors this factor is fully functional upon cell surface expression such as on monocytes It contains three distinct domains extracellular transmembrane and cytoplasmic Expression is induced in platelets and monocytes under procoagulatory and proinflammatory stimuli It plays a major role in HIV-associated coagulopathy and is linked to COVID-19 severity and mortality via platelet-dependent monocyte expression This protein is unique in the coagulation pathway as no congenital deficiency has been reported Alternative splicing results in multiple transcript variants encoding different isoforms
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This kit detects F5 (Human coagulation factor ) in serum plasma and tissue homogenates using a quantitative sandwich ELISA method It offers a detection range of 3 12 ng/mL to 200 ng/mL with a sensitivity of 0 78 ng/mL enabling precise measurement of F5 concentrations in biological samples The assay requires a sample volume of 50-100 L and can be completed within 1-5 hours Detection is performed at 450 nm ensuring reliable and specific results for research or diagnostic applications Designed for flexibility this kit provides a standardized approach to analyze coagulation factor levels across multiple sample types while maintaining high reproducibility and accuracy
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Diluent B for Interference Testing (25 mL) is the special diluent used in Interference Test Kit (K2010001) This diluent should be spiked into the control specimen when testing the interference of Free Bilirubin It can also be used to dilute the stock solution of free bilirubin For in vitro research use only
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