Simply add ethanol to a TRI Reagent sample, bind directly to the Zymo-Spin™ Column, wash, and elute RNA. No phase separation, precipitation, or post-purification steps are necessary.RNA is high-quality and ready for Next-Gen Sequencing, RTqPCR, transcription profiling, hybridization, etc.Features:1. Efficient recovery of DNA-free Total RNAHigh-quality DNA-free RNA is purified from human epithelial cells using the Direct-zol™ procedure compared to a preparation from Supplier Q.2.Complete miRNA recoveryMicroRNA isolation can use Direct-zol™ RNA kits.The data show RNA purified from TRIzolsamples using the Direct-zol™ RNA MiniPrep compared to a method known to be unbiased2 (mirVana™, Ambion).MicroRNA analysis was performed using miRNAseq (MiSeq, Illumina) and direct hybridization assay (nCounter, Nanostring).