DNA Extraction and Purification
MDI Membrane FAST ENDOFREE GIGAPREP 2RXN
Endotoxin free Gigaprep pDNA purification kits are certified for low endotoxin levels of < 0.1 EU/µg. These are specially designed to offer very high yields and purity with much reduced process time. The kit allows fast purification of large quantities of plasmid DNA, from culture pellet, in just 50 minutes. The high binding capacity Giga column with special membrane allows elution of highly concentrated plasmid DNA and does not require DNA precipitation and reconstitution, preventing yield losses and salt contamination.
Zymo Research Corporation DNA Clean & Concentrator™-100 (50 Preps) w/ Zymo Spin V
The DCC-100 employs a single-buffer systemthat allows for efficient DNA adsorption onto the matrix of the supplied Zymo-Spin™ V Columns. Simply add the specially formulated DNA Binding Buffer to your sample and transfer the mixture to the supplied Zymo-Spin™ V Column with Reservoir. There is no need for organic denaturants or chloroform. The purified DNA is ideal for DNA ligation, sequencing, labeling, PCR, microarray, transfection, transformation, and restriction digestion procedures. The entire DNA purification/concentration procedure typically takes less than 20 minutes and can be performed using a centrifuge or vacuum source together with a microcentrifuge.
Zymo Research Corporation EZ DNA Methylation-Gold™ Kit (200 Rxns)
The EZ DNA Methylation-Gold Kit is a refinement of our popular EZ DNA Methylation kit and uses heat denaturation instead of chemical denaturation of the input DNA. This allows for the denaturation and bisulfite conversion steps to be consolidated into one step, leading to a much-reduced incubation time. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
Zymo Research Corporation DNA Clean & Concentrator™-100 (25 Preps) w/ Zymo Spin V
The DCC-100 employs a single-buffer systemthat allows for efficient DNA adsorption onto the matrix of the supplied Zymo-Spin™ V Columns. Simply add the specially formulated DNA Binding Buffer to your sample and transfer the mixture to the supplied Zymo-Spin™ V Column with Reservoir. There is no need for organic denaturants or chloroform. The purified DNA is ideal for DNA ligation, sequencing, labeling, PCR, microarray, transfection, transformation, and restriction digestion procedures. The entire DNA purification/concentration procedure typically takes less than 20 minutes and can be performed using a centrifuge or vacuum source together with a microcentrifuge.
Biochain Institute Inc Genomic DNA - Human Diabetic Diseased Tissue: Stomach, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Express Cloning Checker Kit I: Red Solution, 1 ml/PK
BioChain's Express Cloning Checker Kits provide highly efficient methods of identifying recombinant colonies after transformation eliminating time-consuming plasmid preparation. In the large-scale screen method, the partial colonies of E. coli bacteria can be directly transfered from the transformation plates into the solutions of the kits and immediately run on an agarose gel electrophoresis. The results will be known within 1-2 hours instead of the 2 days required in a conventional method of labor-intensive plasmid DNA miniprep or colony hybridization.
Lonza Walkersville TheraPEAK ACK Lysing Buffer 1x
Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used to lyse red blood cells in preparations containing white blood cells Examples include bone marrow buffy coats whole blood treated with EDTA All TheraPEAK Products are produced according to applicable GMP standards and follow the USP/EP guidance for cell and gene therapy raw materials It is the end user s responsibility to ensure full compliance with local regulations in terms of safety assessments and effective removal prior to patient exposure TheraPEAK Media Products are produced according to applicable GMP raw material standards and are intended to support GMP manufacturing TheraPEAK Media Products are produced at FDA registered manufacturing sites with an ISO 13485 certified quality management system
Agilent Technologies RECOVEREASE DNA ISOLATION KIT
RecoverEase DNA Isolation Kit recovers high-molecular-weight (100 to 500 kb) genomic DNA from whole tissue. It is ideal for DNA isolation for transgenic mutation assays.No organic extractions or ethanol precipitations are required.
Zymo Research Corporation Quick-DNA Fecal/Soil Microbe MidiPrep™ Kit (25 Preps)
The Quick-DNA Fecal/Soil Microbe Midiprep Kit is designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.
![Zymo Research Corporation Zymoprep™ -96 Yeast Plasmid Miniprep (4 x 96 preps) [Includes E1004 x 4: Zymolyase (2 x 2000 Units Lyophilized) w/ Storage Buffer (500µl)]](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00062073-D2005ZYMOPREPYEASTPLASMIDMINI.JPG-250.jpg)
Zymo Research Corporation Zymoprep™ -96 Yeast Plasmid Miniprep (4 x 96 preps) [Includes E1004 x 4: Zymolyase (2 x 2000 Units Lyophilized) w/ Storage Buffer (500µl)]
The Zymoprep™-96 Yeast Plasmid Miniprep kit provides all the necessary reagents for high-throughput plasmid isolation from S. cerevisiae, C. albicans, S. pombe and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The Zymoprep™-96 is a simple and efficient yeast plasmid miniprep kit that is based on the classic E. coli alkaline lysis method with our Zymolyase™ added in the first solution. There is no need for glass beads, or phenol.
Zymo Research Corporation ZymoPURE™ Wash 1 (55 ml)
ZymoPURE™ Wash 1 (55 ml). Assists in washing ZymoPURE™ columns for subsequent purification of nucleic acid.
Zymo Research Corporation ZymoPURE Plasmid MiniPrep Kit (800 Preps)
ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 g/l).In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.As an added convenience, the ZymoPURE™ Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Zymo Research Corporation EZ DNA Methlyation-Direct™ Kit (200 Rxns)
The EZ DNA Methylation-Direct kit is a bisulfite conversion kit that features reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this bisulfite conversion kit makes it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Recovered bisulfite-converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
Biochain Institute Inc Genomic DNA - Mouse Normal Tissue: Brain, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Plant Normal Tissue: Rice, 10 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.