Miscellaneous Molecular Biology Reagents
New England Biolabs, Inc. O-Glycosidase – 2000000 units
O-Glycosidase, also known as Endo--N-Acetylgalactosaminidase, catalyzes the removal of Core 1 and Core 3 O-linked disaccharides from glycoproteins.
New England Biolabs, Inc. SspI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence AAT_^ATT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. Endonuclease IV – 5000 units
Endonuclease IV can act on a variety of oxidative damage in DNA. The enzyme is apurinic/apyrimidinic (AP) endonuclease that will hydrolyse intact AP sites in DNA. AP sites are cleaved at the first phosphodiester bond that is 5' to the lesion leaving a hydroxyl group at the 3' terminus and a deoxyribose 5'-phosphate at the 5' terminus. The enzyme also has a 3' -diesterease activity and can release phosphoglycolaldehyde, intact deoxyribose 5'-phosphate and phosphate from the 3' end of DNA.
New England Biolabs, Inc. EcoRV-HF – 4000 units
A high fidelity restriction endonuclease that recognizes the sequence GAT_^ATC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
Bioworld Hybridization Cocktail, 0% Formamide, 100 mL
Hybridization Cocktail, 0% Formamide Speciffically designed to optimize complementary base-pairing of DNA and RNA in Southern Blot, Northern Blot, Dot Blot, Colony and in situhybridizations. The components of the Hybridization Cocktail will stabilize the target and probe nucleic acids, lower melting temperatures, concentrate the nucleic acids in solution and lower background on the membrane or slides. Ref: Unger E.R., Budgeon L.R., Meyerson D., Brigati D.J., Am. J. Surg. Pathol., 10(1): 1-8 (1986). 0% Formamide RNase/DNase: None Detected
ProteinSimple Maurice CE-SDS PLUS Application Kit
The CE-SDS PLUS application kit contains all components required to run CE-SDS PLUS application. The kit includes CE-SDS PLUS Cartridges, Separation Matrix, Running Buffer, CE-SDS PLUS Sample Buffer, Wash solution, Conditioning Solutions, Internal Standard, Reagent Vials, Pressure Caps and 96-Well Plates. Molecular weight markers can be ordered separately.
Fluidigm Corp Cell-ID™ Intercalator-Rh, 2000 µM
Cell-ID Intercalator-103Rh is a cationic nucleic acid intercalator that contains natural-abundance rhodium (103Rh) and is used in CyTOF analysis for discrimination of dead cells from live cells (if staining is done before cell fixation) or to discriminate single nucleated cells from doublets (if staining is done post-fixation). It is a live cell membrane-impermeable dye. For CyTOF2 and Helios™ instruments we recommend using Cell-ID Intercalator-Ir (201192B) for nucleated cell discrimination and Cisplatin-194 (201194) or -198 (201198) for dead cell discrimination.
Kd Medical Inc 3M POTASSIUM ACETATE BOTTLE
3M Potassium Acetate Solution, pH5.2, is used for preparation of BAC and PAC DNA. This solution is a sterile stock. One 100ml bottle.
Kd Medical Inc SODIUM CITRATE, 1M, 100ML BTL
Buffering agent used in RNA isolation. One 100ml bottle.
Cambridge Isotope Laboratories Melamine 100ug/mL in water (13C3, 99%; Amino-15N3, 98%) | 108-78-1 | MFCD00006055 | 1.2 mL
Melamine 100ug/mL in water (13C3, 99%; Amino-15N3, 98%) | MW: 132.08 | 108-78-1 | MFCD00006055 | 1.2 mL
Biochain Institute Inc Total RNA - Rat Normal Tissue: Skeletal Muscle, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.