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Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
GoldBio s GB10B-Pro Electrocompetent E coli cells are equivalent to DH10B cells and are especially designed for the most demanding cloning applications GB10B-Pro cells will provide the greatest number of transformants for when your research requires it including assembling large and multi-DNA fragments cloning large ( 10 kb up to 350 kb) or difficult construct transformations working with synthetic bio-applications and even BAC cloning
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GoldBio s GV3101 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) harboring the gentamicin resistance A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted in the Ti plasmid and instead has a binary vector containing the missing T-region The binary system makes possible to transfer genetic material into a host plant s genome Our system is often used for Agrobacterium -mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana tobacco potato soybeans and corn
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GoldBio s C58C1 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction C58C1 has the chromosomal backbone of C58 but it has been cured of the Ti plasmid pTiC58 Our C58C1 strain harbors streptomycin and rifampicin resistance genes C58C1 Competent cells are optimized for genetic transformation of plants such as Arabidopsis but can be used in many other plants
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GoldBio s RR1 chemically competent E coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning E coli RR1 is a recA derivative of the HB101 strain and can be more useful than HB101 if a recA background is required
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GoldBio s RR1 chemically competent E coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning E coli RR1 is a recA derivative of the HB101 strain and can be more useful than HB101 if a recA background is required
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GoldBio s GV3101 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) harboring the gentamicin resistance A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted from the Ti plasmid and instead has a binary vector containing the missing T-region The binary system makes possible to transfer genetic material into a host plant s genome Our system is often used for Agrobacterium -mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana tobacco potato soybeans and corn
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GoldBio s GB10B Chemically Competent E coli cells are equivalent to DH10B cells GB10B cells are high efficiency and suitable for a wide variety of applications such as cloning and sub-cloning GB10B Chemically Competent E coli cells have multiple features including the 80lacZ M15 marker which provides -complementation of the -galactosidase gene with blue/white screening protocol These cells also have the mcrA genotypic marker and the mcrBC mrr deletion which allows for cloning of DNA that contains methylcytosine and methyladenine
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GoldBio s AGL-1 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its rec A recombination gene This mutation stabilizes recombinant plasmids AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted from the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region The binary system makes possible to transfer genetic material into a host plant S genome Therefore our system is often used for Agrobacterium -mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana maize and other plants
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GoldBio s LBA4404 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction Our LBA4404 strain harbors a rifampicin resistance (rif) gene Furthermore LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function containing the vir genes Our LBA4404 strain can be used in genetic transformation of tomato tobacco and other plants
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GoldBio s GV3101 Agrobacterium electrocompetent cells are a powerful tool for high efficiency transformation of plants The GV3101 strain is optimized for cDNA and gDNA library construction and possesses resistance to rifampicin gentamicin and tetracycline The T-DNA region of the GV3101 Ti plasmid has been deleted but restoration of the binary vector T-region can fully enable T-DNA binary system function
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GoldBio s EHA105 Electrocompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function containing the vir gene EHA105 is ideal for genetic transformation of rice tobacco and several other plants
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GoldBio CJ236 Electrocompetent E coli cells are ideally suited for use in the Kunkel method of mutagenesis CJ236 cells have part of their Thymine replaced with deoxyuracil in the DNA due to dut - and ung - mutations which prevent the cells from removing uracil from DNA caused by misincorporation or dCTP deamination CJ236 contains the F gene which confers Chloramphenicol resistance
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GoldBio s new Auxo-Agro competent cells are methionine auxotrophic strains of Agrobacterium which reduce overgrowth during the infection process while increasing plant transformation efficiency
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GoldBio s GB10B Electrocompetent E coli cells are equivalent to DH10B cells GB10B cells are high efficiency 2 x 10 8 cfu/ g plasmid DNA suitable for a wide variety of applications requiring high transformation efficiencies such as cloning and subcloning as well as working with either cDNA or gDNA library construction GB10B Electrocompetent E coli cells have multiple features including the 80lacZ M15 marker which provides -complementation of the -galactosidase gene with blue/white screening protocol These cells also have the mcrA genotypic marker and the mcrBC mrr deletion which allows for cloning of DNA that contains methylcytosine and methyladenine
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EHA105 (pSoup) Electrocompetent Agrobacterium cells are designed to provide high transformation efficiencies for applications such as cDNA or gDNA library construction The strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) which carries the vir gene necessary for the insertion of T-DNA into the plant genome Although the pEHA105 plasmid is incapable of transferring its own T-DNA it can still transfer foreign binary vector T-DNA
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