Competent Cells
Invitrogen™ One Shot™ BL21 Star™ (DE3) Chemically Competent E. coli
One Shot BL21 Star (DE3) Chemically Competent E. coli are designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7-promoter-based expression systems such as Champion pET vectors.
Invitrogen™ One Shot™ BL21-AI™ Chemically Competent E. coli
One Shot BL21-AI Chemically Competent E. coli cells are designed for applications that require tight regulation and strong expression of toxic proteins from any T7-promoter-based expression systems.
Invitrogen™ One Shot™ PIR2 Chemically Competent E. coli
One Shot PIR2 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).
Invitrogen™ MultiShot™ StripWell TOP10 Chemically Competent E. coli
MultiShot StripWell TOP10 Chemically Competent E. coli cells are ideal for high-efficiency cloning and plasmid propagation, achieving a transformation efficiency of >1 x 108 cfu/μg with control plasmid DNA in StripWell format.
Invitrogen™ One Shot™ PIR1 Chemically Competent E. coli
One Shot PIR1 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).
MilliporeSigma™ Novagen™ Rosetta-gami™ B Competent Cell Set
Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance the expression of eukaryotic proteins. Contains the pLacI plasmid producing extra Lac repressor.
MilliporeSigma™ Novagen™ BL21 (DE3) pLysS Singles™ Competent Cells
BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.
MilliporeSigma™ Novagen™ Tuner™ (DE3) pLacI Competent Cells
Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture. Contains the pLacI plasmid producing extra Lac repressor.
MilliporeSigma™ Novagen™ Rosetta™ Blue Competent Cells
RosettaBlue host strains are NovaBlue derivatives that combine high transformation efficiency and with enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
MilliporeSigma™ Novagen™ DE3 Competent Cell Set
For Protein Expressions
MilliporeSigma™ Novagen™ Tuner™ DE3 pLysS Competent Cells
Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture. T7 lysozyme expression suppresses basal T7 expression.
MilliporeSigma™ Novagen™ Origami™ 2 (DE3) pLysS Competent Cells
Origami 2 strains have mutations in glutathione reducatase(gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation. T7 lysozyme expression suppresses basal T7 expression.
Invitrogen™ One Shot™ BL21(DE3)pLysE Chemically Competent E. coli
One Shot BL21(DE3)pLysE Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET.
MilliporeSigma™ Novagen™ Rosetta™ 2(DE3)pLacI Competent Cells
Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.
Invitrogen™ MultiShot™ StripWell Stbl3™ Competent Cells
MultiShot StripWell Stbl3 Chemically Competent E. coli cells are cloning competent cells designed for cloning direct repeats found in lentiviral expression vectors.