Protein Labeling Reagents
Biotium CF633 Protein Labeling Kit , 3x(1mg) labelings
This kit contains everything you need to label antibodies or other proteins with a fluorescent CF dye succinimidyl ester (CF dye SE). Each kit contains reagents for three labeling reactions of up to 1 mg protein per reaction. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF633 dye has excitation/emission at 630/650 nm.
Biotium Tyramide Amplification Kit with HRP Streptavidin and Biotin-XX Tyramide
Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure. The kit contains HRP streptavidin Biotin-XX tyramide. The kit contains sufficient reagent for 50-150 slides.
Biotium 5-TAMRA-PEO3-amine
5-TAMRA-PEO3-amine (Tetramethylrhodamine-PEO3-amine) has a long water-soluble PEG spacer separating the dye and the amine functional group. The dye can be coupled to activated carboxy groups or sulfonyl chlorides. Properties: Ex/Em (MeOH) = 544/571 nm; Dark purple solid soluble in DMSO, DMF, or H2O; Store at 4°C and protect from light; C 37 H 42 F 6 N 4 O 11; MW: 832.74
Biotium Fura-2, Pentapotassium Salt
Fura-2, pentaammonium salt is a ratiometric fluorescent dye that binds to free intracellular calcium. It is membrane-impermeant and may be loaded into cells via microinjection or scrape loading. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2-free) to 335 nm (Ca2-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm.Properties: Light yellow solid soluble in DMSO; Store at -20°C, protect from light, especially in solution; Kd: 145 nM (22°C, pH 7.2 buffer); Ex/Em = 363/512 nm, e = 27,000 M-1cm-1 (no Ca2); Ex/Em 335/505 nm, e = 35,000 M-1cm-1 (high Ca2); C29H22K5N3O14; MW: 832; [113694-64-7].
Biotium 5-(and-6-)-((N-(5-aminopentyl)amino)carbonyl) Tetramethylrhodamine (Tetramethylrhodamine cadaverine) (mixed isomer)
INA (5-iodonaphthyl-1-azide) is a lipophilic photoreactive probe, which has been used to selectively label membrane-embedded cysteine residues of proteins. Properties: Light yellow solid soluble in DMSO or DMF; Store at 4°C and protect from light; C30H34N4O4; MW: 295.08.
Biotium Phalloidin, CF568, 300 U
Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Red fluorescent CF568 has excitation/emission at 562/583 nm.
Biotium GloMelt™ Thermal Shift Protein Stability Kit
GloMelt™ dye undergoes fluorescence enhancement upon binding to hydrophobic regions of denatured proteins. Therefore the dye can be used to detect protein unfolding or measure thermal stability by performing a thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay. GloMelt™ dye has significant advantages over other environmentally sensitive dyes, such as SYPRO Orange and PROTEOSTAT TS dye. GloMelt™ dye generates a strong signal because it is optimized for detection in the SYBR Green channel of qPCR instruments, and therefore low reaction volumes and low protein concentrations can be used. GloMelt™ dye is compatible with high concentrations of protein stabilizers (such as glycerol and sorbitol), and also protein destabilizers. GloMelt™ dye performs very well in high detergent concentrations, unlike SYPRO Orange. This kit contains no ROX reference dye and has sufficient reagent for 2000 assays.
Biotium Tyramide Amplification Kit with HRP Goat Anti-Rabbit and CF568 Tyramide
Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure. The kit contains HRP goat anti-rabbit CF568 dye tyramide. The dye has an excitation/emission of 562/583 nm. The kit contains sufficient reagent for 50-150 slides.
Aat Bioquest ICG-Sulfo-OSu
Indocyanine green (ICG) is a cyanine dye used in medical diagnostics. It is used for determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography. It has a peak spectral absorption close to 800 nm.
STEMCELL Technologies GloCell™ Fixable Viability Dye UV 450, Size: 100 Tests
GloCell™ Fixable Viability Dye UV 450 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.GloCell™ Fixable Viability Dye UV 450 is excited by the UV laser at 355 nm and it has a fluorescence emission maximum of 450 nm that can be detected using the 450/50 band pass filter used to detect DAPI, Hoechst 33258, etc.
Apexbio Technology LLC Cy3 NHS ester, 5mg. Cas: MFCD: MFCD28505569. Labeling of amino-groups in biomolecules.
Sulfo-Cy3 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For labeling proteins and peptides, no organic co-solvent is needed. Other sizes are available. Please inqury us for quote.
AdipoGen 5-TAMRA N-succinimidyl ester
Chemical. CAS 150810-68-7. Formula C29H25N3O7. MW 527.52. Synthetic. Amine-reactive form of 5-carboxytetramethylrhodamine single isomer. Conjugates yield bright, pH-insensitive orange-red fluorescence Ex/Em ~546/579nm with good photostability.
Cytoskeleton, Inc. SIR-LYSOSOME KIT
SiR-lysosome Kit (CY-SC012) live cell fluorogenic lysosome labeling probe