Protein Assays
Indigo Biosciences Human MR Reporter Assay Kit, 1 x-96 well format assays
NC3798067 HUMAN MR REPORTER ASSAY 1X96 W
Abcam Trypsin Activity Assay Kit (Colorimetric)
Trypsin Activity Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
BIOHIPPO EF1a-H3.3-K27M-Flag/BSD
EF1a-H3 3-K27M-Flag/BSD lentiviral vector expressing Flag under EF1a promoter Each vial provides a nominal titer of 2x10E6 transducing units TU Human H3 3 K27M ORF cDNA lentivirus Lentiviral particles packaged with expression vector for K27M mutant of human H3 3 histone A (also known as H3 3 or H3-3A) transcript variant 1 An EF1 promoter drives expression of H3 3 K27M fused to a C-terminal V5 tag and a GFP reporter which is separated from the ORF cDNA by a self-cleaving peptide These lentiviral particles allow for easy transduction and high stable expression of the indicated ORF cDNA in mammalian cells The lentiviral vectors have been optimized to provide high gene expression and reliable genome integration allowing for easy establishment of long-term stable cell lines with consistent read out of fluorescent (GFP RFP) or luminescent (firefly luc) reporters
Sigma Aldrich Fine Chemicals Biosciences Lactate Dehydrogenase Inhi
A cell-permeable quinolinesulfonamide compound that acts as potent and reversible inhibitor of lactate dehydrogenase A (IC50 2.6 nM for human LDH-A). The inhibition appears to be competitive with respect to NADH and non-competitive with respect to pyruvate. Exhibits high selectivity over LDH-B (IC50 43 nM) and does not affect the activity of 32 other enzymes receptors and ion-channels even at high concentration (~ 10 181M). Rapidly reduces lactate production in hepatocellular (EC50 400 and 588 nM in Snu398 and HepG2 cells respectively) and breast carcinoma cell lines and induces PKM2 activation (EC50 600 nM in Snu398 cells). Shown to increase mitochondrial oxygen consumption rate (EC50 500 and 900 nM in Snu398 and HepG2 cells respectively) and causes a diminution in extracellular acidification rates (EC50 600 nM in both cell lines). Shown to downregulate the ability of A549 cells to form tumorspheres and diminishes the number of CD24/CD44-positive cells in HMLER system and i