Bioluminescence Assays and Reagents
Indigo Biosciences Human RAR-related Orphan Receptor alpha (RORa, NR1F1) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human RAR-related Orphan Receptor Alpha reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ROR alpha (NR1F1). Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Indigo Biosciences Human Estrogen Receptor alpha (ERa, NR3A1) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Estrogen Receptor Alpha reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ER Alpha (NR3A1). Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Indigo Biosciences Human Vitamin D Receptor (VDR, NR1I1) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Vitamin D Receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against VDR (NR1I1). Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Indigo Biosciences INDIGlo LDR Kit 50 mL of 2x- concentration Luciferase Detection Reagent and Dilution Buffer
INDIGlo Luciferase Detection Reagent from INDIGO Biosciences is a glow type luciferase detection reagent designed for the quantification of firefly and beetle luciferase expression in mammalian cells. The INDIGlo Luciferase Detection Reagent kit is formulated as a 2x-concentrated reagent which contains both the Luciferase Detection Reagent (LDR) and Dilution Buffer and can be used for both homogenous and non-homogenous assay applications. With a signal duration of greater than 2 hours and with a less than 10% fluctuation in signal, the highly sensitive and stable luminescence signal of INDIGlo is ideal for the cost-effective processing of cell culture plates ranging from 96 to 1536-well format.
Apexbio Technology LLC Firefly Luciferase mRNA (ARCA, 5mCTP, UTP), 5x1mg (1mg/mL)
Firefly Luciferase mRNA (ARCA, 5mCTP, UTP) expresses luciferase protein which is initially extracted from firefly Photinus pyralis. The enzyme catalyzes ATP-dependent D-luciferin oxidation to yield oxyluciferin, a singlet-excited compound that emits light when returning to its ground state. Firefly Luciferase is a frequently used bioluminescent reporter for gene regulation and function study. It is applicable in assays for gene expression, cell viability and in vivo imaging etc. Our Firefly Luciferase mRNA is provided at a concentration of 1mg/ml, it is ARCA capped, polyA-tailed and incorporated with modified nucleotides. The capping of ARCA (anti-reverse cap analog) ensures high translation efficacy, while the addition of 5mCTP/UTP and poly(A) tail reduces host cell immune response and enhance mRNA stability. Other sizes are available. Please inqury us for quote.
Revvity Health Sciences Inc sensilite Enhanced Flash Luminescence (Firefly Luciferase) Reporter Gene Assay System, 100 Assay Points
sensilite Enhanced Flash Luminescence (Firefly Luciferase) Reporter Gene Assay System, 100 Assay Points
Abcam WAY 262611, Wnt pathway activator, 5MG
MW 318.4 Da, Purity >97%. Wnt pathway activator. Selectively inhibits Dkk1 with no GSK-3β inhibition. TCF-Luciferase agonist (EC₅₀ = 0.63 μM). Increases bone formation rate in vivo. Orally active.
The product is subject to the following: Abcam Restricted Use Statement
Abcam D-Luciferin, chemiluminescent luciferase substrate, 10MG
MW 280.3 Da, Purity >98%. Cell-permeable chemiluminescent luciferase substrate. Emits lights upon oxidative decarboxylation in the presence of ATP. Provides a bioluminescent signal for cellular ATP levels in vivo. Water soluble analogs are D-Luciferin potassium salt (ab143655) and D-Luciferin sodium salt (ab145164).
The product is subject to the following: Abcam Restricted Use Statement
Apexbio Technology LLC Firefly Luciferase mRNA (ARCA, 5-moUTP), 5x1mg (1mg/mL)
Firefly Luciferase mRNA (ARCA, 5-moUTP) expresses luciferase protein which is initially extracted from firefly Photinus pyralis. The enzyme catalyzes ATP-dependent D-luciferin oxidation to yield oxyluciferin, a singlet-excited compound that emits light when returning to its ground state. Firefly Luciferase is a frequently used bioluminescent reporter for gene regulation and function study. It is applicable in assays for gene expression, cell viability and in vivo imaging etc. Our Firefly Luciferase mRNA is provided at a concentration of 1mg/ml, it is ARCA capped, polyA-tailed and incorporated with modified nucleotides. The capping of ARCA (anti-reverse cap analog) ensures high translation efficacy. Poly (A) tail plays an important role in enhancing the efficiency of translation initiation. Other sizes are also available. Please inqury us for quote.
Apexbio Technology LLC EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP), 5x1mg (1mg/mL)
EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP) will express luciferase protein once entering cells which is initially extracted from firefly Photinus pyralis . This enzyme catalyzes ATP-dependent D-luciferin oxidation and lead to yield chemiluminescence at about 560 nm wavelength. Firefly Luciferase is a frequently used bioluminescent reporter for gene regulation and function study. It is applicable in assays for mRNA delivery, translation efficiency, cell viability and in vivo imaging etc. Other sizes are also available. Please inqury us for quote.
Apexbio Technology LLC EZ Cap™ Firefly Luciferase mRNA, 5x1mg
EZ Cap™ Firefly Luciferase mRNA will express luciferase protein once entering cells, which is initially extracted from firefly Photinus pyralis. This enzyme catalyzes ATP-dependent D-luciferin oxidation and lead to yield chemiluminescence at about 560 nm wavelength. Firefly Luciferase is a frequently used bioluminescent reporter for gene regulation and function study. It is applicable in assays for mRNA delivery, translation efficiency, cell viability and in vivo imaging etc.EZ Cap™ Firefly Luciferase mRNA is provided at a concentration of 1mg/ml. It is co-transcriptional capped by EZ Cap™ Reagent AG (3' OMe) (Catalog No.B8178) which generates a cap 1 structure with high efficiency. Cap 1 structure is more ideal for mammalian systems and possesses higher transcription efficiency than Cap 0 structure (ARCA and mCap). Other sizes are also available. Please inqury us for quote.
Biotium Coelenterazine Sampler Kit
For researchers wishing to screen for the appropriate coelenterazine derivatives, the sampler kit includes 25 ug each of nine coelenterazine analogs including native, cp, f, fcp, h, hcp, i, ip and n.Coelenterazines are luminescent enzyme substrates for apoaequorin and Renilla luciferase. Apoaequorin is used as a calcium indicator. It binds coelenterazine to form an aequorin complex that emits light upon calcium binding and measures concentrations ~0.1 uM to >100 uM. Renilla luciferase is widely used as a reporter of transcription regulation and as a donor in bioluminescence resonance energy transfer (BRET) to study protein-protein interactions. Coelenterazines are also used for chemiluminescent detection of superoxide anions and peroxynitrite in cells or tissues. Coelentrazine analogs and water-soluble Aquaphile™ Coelenterazines are also available.Properties : Yellow solids soluble in MeOH or EtOH; Store at -20°C, protect from light.
Cayman Chemical CyprIdIna LucIfRIntrIfluor 1mg
A biotinylated and PEGylated form of cholesterol has been used as a lipid anchor to immobilize vesicles liposomes viral particles bacteria or yeast to microfluidic chips increases phycoerythrin-conjugated streptavidin density on the surface of mesenchymal stem cells greater than sulfo-NHS-LC-biotin at 10 UM
Cayman Chemical Cy5 FIrefly LucIfRase mRNA
A cardenolide with diverse biological activities inhibits N-acetyltransferase (IC50 ~1.2 UM) reduces maximal Na /K -ATPase pump activity and cell attachment and increases cell death in MDCK-C7 cells at 1 UM decreases the levels of SRC-3 in MCF-7 cells at 500 nM increases calcium efflux and sarcoplasmic reticulum calcium levels in a voltage-clamp assay using isolated and perfused guinea pig hearts at 50 UM
Cayman Chemical FIrefly LucIferase mRNACap 1mg
An ETB receptor agonist selectively binds to ETB over ETA receptors (IC50s 0.33 and 570 nM respectively for the porcine receptors) increases wound closure percentage in a scratch assay using HUVECs at 10 or 100 nM increases body temperature in conscious rabbits at 0.15 or 0.3 nmol/kg induces bronchoconstriction and increases mean arterial blood pressure in anesthetized guinea pigs at 20 nmol/kg i.v. has been used to determine the cross reactivity of monoclonal antibodies against endothelin in enzyme immunometric assays