General Cell Based Assays
Eurofins Discoverx AssayComplete Cell Plating 14 Reagent, 100. (93-0563R14A)
AssayComplete Cell Plating 14 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress CHRM5 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0519E2CP0M)
The PathHunter CHRM5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. oxotremorine) based activation of CHRM5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM5 and EA-tagged β-Arrestin-2. Activation of CHRM5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Apexbio Technology LLC Live-Dead Cell Staining Kit, 500T. Cas: MFCD: NA.
Our product Live-Dead Cell Staining Kit is used for simultaneous fluorescence staining of viable and dead cells. This kit contains two dyes - Calcein-AM and Propidium Iodide (PI), which stain viable and dead cells, respectively.
Eurofins Discoverx PathHunter eXpress TrkA Functional Assay, 10-Plate Kit. (93-0462E3CP16L)
The PathHunter eXpress TrkA Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkA dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkA and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress MRGPRX2 CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0309E2CP0L)
The PathHunter MRGPRX2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. coristatin-14) based activation of MRGPRX2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MRGPRX2 and EA-tagged β-Arrestin-2. Activation of MRGPRX2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Thawing Reagent T8, 100. (92-4108TR)
AssayComplete Thawing Reagent T8 is an optimized thawing reagent for revival and recovery of Eurofins DiscoverX cell lines. This reagent ensures optimal post-thaw viability, recovery and assay performance upon thawing. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended thawing reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress TrkB Functional Assay, 10-Plate Kit. (93-0463E3CP16L)
The PathHunter eXpress TrkB Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkB dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkB and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress F2RL1 (PAR2; GPR11) HEK 293 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0235E1CP0M)
The PathHunter F2RL1 (PAR2; GPR11) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. proteases, SLIGRL) based activation of F2RL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged F2RL1 and EA-tagged β-Arrestin-2. Activation of F2RL1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Freezing Reagent F6, 2 x 50. (92-5106FR)
AssayComplete Freezing Reagent F6 is an optimized freezing reagent for cryopreservation of Eurofins DiscoverX cell lines. This reagent ensures optimal cell viability and assay performance upon thawing. This product is provided as 2 bottles of 50 mL reagent each. Please refer to cell line specific datasheet to determine the recommended freezing reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress PTGFR U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0936E3CP5M)
The PathHunter PTGFR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. cloprostenol) based activation of PTGFR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGFR and EA-tagged β-Arrestin-2. Activation of PTGFR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter SIRPa Signaling Bioassay Kit, 2pl. (93-1135Y19-00129)
The PathHunter SIRPa Signaling Bioassay consists of human cells engineered to measure drug potency and detect neutralizing antibodies. Engagement of the receptor by its ligand, or agonist antibodies results in phosphorylation of the receptor’s cytosolic tail. The SH2-domain fused to EA binds the phosphorylated receptor, forcing complementation of ED and EA, resulting in formation of an active β-gal enzyme, which hydrolyzes the substrate to generate a chemiluminescent signal. The bioassay kit contains all reagents needed for the assay including pre-qualified cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress OSMRb/IL31RA Dimerization Assay, 2pl Kit. (93-1002E3CP5M)
The PathHunter OSMRb/IL31RA Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-31) induced dimerization of OSMRb/IL31RA receptors. This product uses EFC technology. The cell line included overexpresses OSMRb target protein fused to PK alongside IL31RA target protein fused to EA. Upon ligand-induced dimerization of OSMRb/IL31RA, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress Keap1-NRF2 Nuclear Translocation Assay, 10-plate Kit. (93-0821E3CP0L)
The PathHunter eXpress Keap1-NRF2 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the Keap1-NRF2 pathway. This kit assesses ligand (e.g. methyl CDDO) based pathway activation via detection of resulting NRF2 nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged NRF2 and nuclear localized EA alongside untagged Keap1. Upon pathway activation, induced nuclear translocation of NRF2 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OXGR1 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0693E2CP1M)
The PathHunter OXGR1 (GPR80, GPR99) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of OXGR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OXGR1 and EA-tagged β-Arrestin-2. Activation of OXGR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.