General Cell Based Assays
Revvity Health Sciences Inc AlphaScreen FITC Detection Kit, 50,000 Assay Points
AlphaScreen FITC Detection Kit, 50,000 Assay Points
Cayman Chemical Triglyceride Colorimetric Assay Kit, 96 Well
Cayman’s Triglyceride Colorimetric Assay Kit provides a simple, reproducible, and sensitive tool for assaying mono-, di-, and triglycerides in plasma, serum, cell culture extracts, and tissue homogenates. The Triglyceride Colorimetric Assay Kit uses the enzymatic hydrolysis of triglycerides by lipase to produce glycerol and free fatty acids. The glycerol released is subsequently measured by a coupled enzymatic reaction system. The glycerol formed in reaction 1 is phosphorylated to glycerol-3-phosphate in a reaction catalyzed by glycerol kinase. The glycerol-3-phosphate is oxidized by glycerol phosphate oxidase producing dihydroxyacetone phosphate and hydrogen peroxide (H2O2). Peroxidase catalyzes the redox-coupled reaction of H2O2 with 4-aminoantipyrine (4-AAP) and N-ethyl-N-(3-sulfopropyl)-m-anisidine (ESPA), producing a brilliant purple color. The absorbance is measured at 540 nm.
STEMCELL Technologies Ascorbic Acid, Size: 100 g
Support the proliferation of mesenchymal stem cells (Choi et al. J Biosci Bioeng, 2008) with Ascorbic Acid. Ascorbic Acid is a reducing agent that shows antioxidant activity against reactive oxygen species. Ascorbic Acid is used to differentiate mouse embryonic stem (ES) cells into osteoblasts (zur Nieden et al. Differentiation, 2003) and cardiomyocytes (Takahashi et al. Circulation, 2003). This small molecule also promotes differentiation of osteoblasts from human and mouse mesenchymal cells (Pittenger et al. Science, 1999; Tropel et al. Exp Cell Res, 2004) and increases the efficiency of reprogramming mouse and human fibroblasts to induced pluripotent stem (iPS) cells (Esteban et al. Cell Stem Cell, 2010). L-Ascorbic Acid is supplied as a crystalline solid with ≥95% purity; Molecular weight 176.1 g/mol; CAS Number 50-81-7. Visit STEMCELL.com for more information and related products.
Enterprise Technology Solutions 3M Laminating Shts,Photo Safe,2-Sided,4-3/8"x6-3/8",5/PK,CL
Self-sealing laminating pouches offer instant, permanent document laminating without heat or hassle. No heat or machine is needed. This photo-size laminating pouch is easy to use. Simply open the sheet, place document on clear film, remove liner and seal around the edges. Photo-safe laminating pouches require no special tools or equipment. They deliver two-sided protection in one application. Ultra-clear design lets important information show through.
Eurofins Discoverx PathHunter Human GPR37 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR37 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR37 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Mouse Glucagon (GCG Receptor) β-Arrestin-1 Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse Glucagon (GCGR) β-Arrestin-1 Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GCGR (GPCR) activity via recruitment of β-Arrestin-1. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress SSTR2 Activated GPCR Internalization Assay, 10pl Kit. (93-0676E2CP0L)
The PathHunter SSTR2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of SSTR2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged SSTR2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting SSTR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery VPLEX GLP1 Total Kit 25 PL
The Meso Scale Discovery V-PLEX GLP-1 Total Kit is an electrochemiluminescent immunoassay for the quantitative measurement of total glucagon-like peptide-1 (GLP-1) in human serum plasma and cell culture supernatants The assay utilizes MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and reproducible results with a broad dynamic range and rapid protocol This kit includes a 96-well plate coated with capture antibody detection antibody conjugated to ruthenium label calibrators and reagents required to perform the assay The lower limit of detection is 1 5 pg/mL with an upper limit of 10 000 pg/mL
Abcam Rhod-4 Calcium Assay kit - Medium Removal No Wash.
Rhod-4 Calcium Assay kit - Medium Removal No Wash.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter MC4R Bioassay Kit, 10-Plate. (93-0211Y3-00086)
The PathHunter MC4R Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. αMSH) based activation of MC4R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MC4R and EA-tagged β-Arrestin-2. Activation of MC4R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Abcam Aldolase Activity Assay Kit (Colorimetric)
Aldolase Activity Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter Human IL-29 (IFNRL1/IL10RB) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human IL-29 (IFNRL1/IL10RB) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of IFNRL1/IL10RB. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Abcam Pyruvate Kinase Assay Kit
Pyruvate Kinase Assay Kit
The product is subject to the following: Abcam Restricted Use Statement