General Cell Based Assays
Eurofins Discoverx PathHunter eXpress CXCR7 Activated GPCR Internalization Assay, 2pl Kit. (93-0738E3CP27M)
The PathHunter CXCR7 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CXCR7 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CXCR7. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CXCR7-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human EGFR/ErbB2 Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human EGFR/ErbB2 Dimerization Stable Cell Line Assay (U2OS) measures ligand-induced dimerization of EGFR/ErbB2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human NMU1R β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human NMU1R β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure NMU1R (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cayman Chemical D-Fructose-1,6-bisphosphate (sodium salt hydrate), Purity: ≥95%, CAS: 81028-91-3, 5 g
An intermediate in carbohydrate metabolism, including glycolysis and gluconeogenesis
ILEX LIFE SCIENCES LLC QuickZyme Sensitive Tissue Collagen Assay Kit, 2 x 96 Assays, 96-well plate format, colorimetric assay for low level collagen analysis (tissue, tissue homogenates samples)
QuickZyme Sensitive Tissue Collagen Assay Kit enables collagen analysis in small tissue samples with low collagen levels. The assay is based on quantitative colorimetric determination of hydroxyproline residues, obtained by acid hydrolysis of collagen. The assay allows quantitative measurement of all types of collagen, species independent. The only hydroxyproline-based assay which shows no matrix effect and no need for further dilution of hydrolysate. No drying of hydrolysate is needed, resulting in a fast and easy (96-well plate format) assay. Application: tissues with low collagen/hydroxyproline content (ex. liver). Sensitivity: 2.4 µM (0.3 µg/ml). Assay-time: < 2 hours. Collagen is a main component of extracellular matrix. Dysregulation in collagen production results in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Thus measurement of collagen production is important in many disease related studies.
Abcam MnTBAP chloride, Supero x ide dismutase (SOD) mimetic, 50MG
MW 879.1 Da. Cell-permeable SOD mimetic. Potent peroxynitrite scavenger. Does not scavenge NO. Shows anti-inflammatory effects and reduces oxidative injury in vivo. Reduces ROS and shows a neuroprotective effect in spinal cord injury.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter Mouse CNR1 (CB1) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse CNR1 (CB1) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CNR1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Mouse CXCL16 (CXCR6) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse CXCL16 (CXCR6) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure mCXCR6 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Abcam WST-8 Assay Kit (Cell Proliferation)
WST-8 Assay Kit (Cell Proliferation)
The product is subject to the following: Abcam Restricted Use Statement
AnaSpec SensoLyte Green Glutaminyl Cyclase Activity Assay *Fluorimetric*, 1 Kit
SensoLyte Green Glutaminyl Cyclase Activity Assay *Fluorimetric*, 1 Kit, The SensoLyte Green Glutaminyl Cyclase Activity Assay Kit provides a convenient, two-step homogeneous procedure for measuring enzyme activity from various sources using a green fluorescence substrate. During the first step, substrate is incubated with Glutaminyl Cyclase or enzyme-containing samples and converted into the pyroglutamate form. Secondly, glutaminyl cyclase developer is added to remove pGlu residue and generate the green fluorophore. It can be detected with excitation at 490 nm and emission at 520 nm. Produced fluorescence is proportional to the enzyme activity. This kit is ideal for high throughput screening (HTS) of glutaminyl cyclase activators and inhibitors. The long wavelength of the green fluorophore is less interfered by autofluorescence of cell components and test compounds. Assay sensitivity is 1.5 ng/mL of active enzyme.
Eurofins Discoverx PathHunter Human CHRM1 β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human CHRM1 β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure CHRM1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Molecular Devices Neurotransmitter Uptake Assay, Fluorescent, Homogeneous, 96/384-Well
The Molecular Devices Neurotransmitter Transporter Uptake Assay Kit is a homogeneous, mix-and-read fluorescent assay for measuring live-cell uptake activity of serotonin, norepinephrine, and dopamine transporters. It supports kinetic and endpoint workflows and is suitable for high-throughput screening and assay development.
- Homogeneous mix-and-read fluorescent format compatible with automation.
- Suitable for kinetic real-time measurements and endpoint assays.
- Compatible with 96-well and 384-well plate formats.
- Evaluation kit configuration includes reagents sufficient for two plates.
- Replaces radioactive uptake assays, reducing disposal and safety concerns.
Eurofins Discoverx PathHunter eXpress IL10RA/IL10RB Dimerization Assay, 2pl Kit. (93-0985E3CP0M)
The PathHunter IL10RA/IL10RB Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-10) induced dimerization of IL10RA/IL10RB receptors. This product uses EFC technology. The cell line included overexpresses IL10RA target protein fused to PK alongside IL10RB target protein fused to EA. Upon ligand-induced dimerization of IL10RA/IL10RB, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GHR-JAK2 Functional Assay, 2-Plate Kit. (93-0829E3CP14M)
The PathHunter GHR-JAK2 Functional assay eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting CTKs. This kit assesses GHR activation via detection of JAK2 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged GHR cytokine receptor, an untagged CTK JAK2 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ADRB1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0488E2CP0M)
The PathHunter ADRB1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. isoproterenol) based activation of ADRB1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged ADRB1 and EA-tagged β-Arrestin-2. Activation of ADRB1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.