General Cell Based Assays
Meso Scale Discovery RPLEX HUM PRAS40 total ASY
The R-PLEX Human PRAS40 Total Assay Kit from Meso Scale Discovery is used to quantitatively measure levels of total PRAS40 protein in human cell and tissue samples The assay utilizes an electrochemiluminescent detection method and provides high sensitivity broad dynamic range and requires low sample input The kit includes a 96-well plate coated with capture antibody detection antibody read buffer sample diluent and vials of calibrator This assay allows for the simple and rapid analysis of PRAS40 protein levels which can provide insight into mTOR pathway biology and related disease states
Meso Scale Discovery R-PLEX Human Trk-A total ASY
The R-PLEX Human TrkA Total Assay Kit from Meso Scale Discovery measures total TrkA in human cell and tissue lysates This electrochemiluminescent immunoassay uses MSD s SULFO-TAG labels and multi-array plate technology for high sensitivity and minimal matrix interference The kit includes a 96-well plate with capture antibody detection antibody with ruthenium label calibrators and necessary reagents Results are available in 3 5 hours using an MSD instrument This assay accurately quantifies total TrkA levels
Meso Scale Discovery R-PLEX Mouse Pentraxin 3 ASY
The Meso Scale Discovery R-PLEX Mouse Pentraxin 3 Assay is a multiplex immunoassay that quantitatively measures mouse Pentraxin 3 in serum plasma and cell culture supernatants This assay utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision The assay requires 50 L or less sample volume and can perform up to 80 tests across two plates The assay has a lower limit of detection of 0 04 ng/mL and an upper limit of 10 000 ng/mL This assay allows for the rapid reproducible quantification of mouse Pentraxin 3 from various biological matrices
Genscript Corporation DPPIV/CD26 His Human
CD26/dipeptidyl peptidase (DPP)IV is a membrane-bound protein found in many cell types of the body and a soluble form is present in body fluids There is longstanding evidence that various primary tumors and also metastases express DPPIV/CD26 to a variable extent
Cayman Chemical Lipid Droplets Assay Dye Extr
A Y-secretase modulator selectively inhibits the production of AB42 in CHO cells expressing wild-type APP (IC50 9.8 nM) over Notch cleavage to NICD (IC50 >15.8 UM) decreases brain levels of AB42 in rats at 10 and 40 mg/kg decreases CSF levels of AB40 and AB42 and increases levels of AB38 and AB39 without affecting total AB CSF levels in dogs at 20 mg/kg
Cayman Chemical HS MInral OIl asy Reagent 15mL
A kainate receptor agonist induces currents in Xenopus oocytes expressing homomeric GluR5 or GluR2 subunit-containing receptors (EC50s 0.16 and 0.23 UM respectively) induces desensitization in HEK293 cells expressing homomeric GluR6 subunit-containing kainate receptors at 0.03-0.3 UM selectively binds to the kainate receptor (IC50 ~0.035 UM) over the AMPA and NMDA receptors (IC50s >100 and 7 UM respectively) but does bind to EAAT2 (Kb 3.4 UM) and inhibits glutamate transport reduces hind paw withdrawal frequency and increases withdrawal latency to mechanical and thermal stimuli in a rat model of capsaicin-induced hyperalgesia at 10 50 and 100 mg/kg
Cayman Chemical LIpoxygenase InhIbItr Screen
Cayman’s Lipoxygenase Inhibitor Screening Assay Kit detects and measures the hydroperoxides produced in the lipoxygenation reaction using a purified LO. The detection reaction is equally sensitive to hydroperoxides at various positions within the fatty acid, and will work with fatty acids of any carbon length. It is thus a general detection method for LO, and can be used to screen libraries of compounds for those which inhibit LO enzymes.Provided in this kit is arachidonate 15-LO purified from soybean, which can be used to screen test compounds. Alternatively, if other purified LOs (provided by the user) are to be used for screening, the included enzyme can be used as a control. Both arachidonic and linoleic acids are included as substrates. The preferred substrate for screening will depend on the LO being tested. It is recommended to use the fatty acid substrate best suited for the LO being tested.
Cayman Chemical HemoglobIn ColorImetrIc asy
Hemoglobin (Hb or Hgb) is a globular protein found primarily in erythrocytes that carries oxygen from the lungs to tissue where it releases oxygen and then returns carbon dioxide (CO2) from the tissue to the lungs. Hemoglobin also binds CO2, carbon monoxide (CO), and nitric oxide (NO). Cayman’s Hemoglobin Colorimetric Assay provides a quick, reliable method for determining total hemoglobin concentration in a variety of biological samples, including blood, tissue homogenates and cell lysates. Unlike the universally accepted reference method of hemoglobin determination, which uses potassium cyanide as a reagent and commonly under-estimates hemoglobin levels, Cayman’s Hemoglobin Colorimetric Assay utilizes an optimized detection reagent which is non-toxic, reporting accurate measurements of total hemoglobin concentrations with an absorbance between 560-590 nm.
Cayman Chemical D-Lactate Stndard 1ea
A clear uncoated flat-bottom 96 well plate for use in colorimetric assays.
American Research Products Inc Rat secreted phospholipase A2
Rat secreted phospholipase A2 sPLA2 ELISA Kit from CUSABIO Detection Range 0 78 ng/mL-50 ng/mL Sensitivity 0 195 ng/mL Sample serum plasma tissue homogenates Method Sandwich
ABclonal Technology LDH-B
This gene encodes the B subunit of lactate dehydrogenase enzyme which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD in a post-glycolysis process Alternatively spliced transcript variants have been found for this gene Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism and that this isoform is localized in the peroxisomes Mutations in this gene are associated with lactate dehydrogenase B deficiency Pseudogenes have been identified on chromosomes X 5 and 13