General Cell Based Assays
Sigma Aldrich Fine Chemicals Biosciences Colorimetric Biotin Assay
Biotinylation is the process of labelling a molecule with a biotin molecule to take advantage of the strong binding and high specificity of biotin and streptavidin for purification or detection applications. Biotinylation is typically used to label proteins or nucleic acid based targets for these applications. Quantitation of biotinylation can be used to optimize the labelling of a given target. Levels of biotinylation can effect detection sensitivity or functionality of a purification product.
Sigma Aldrich Fine Chemicals Biosciences Proteasome 20S Activity As
Protein degradation occurs most commonly through two major mechanisms lysosomal degradation and proteasome mediated degradation. Lysosomal degradation occurs through proteolytic enzyme activity and is fairly non-specific. Proteasomal degradation is targeted for a specific protein through the ubiquitination of the target protein. The target protein is typically poly-ubiquitinated and activates the 26S proteasome toward proteolysis. The proteasome 26S is the most common form of the proteasome and is an ATP-dependent proteolytic complex which contains one 20S (700-kDa) core particle structure and two 19S (700-kDa) regulatory caps.The proteasome is responsible for the recycling of proteins and maintenance of the balance of protein synthesis and degradation. The proteasome is involved with apoptosis DNA repair endocytosis and cell cycle and has been implicated in disease states such as certain types of cancer diabetes mellitus and Alzheimer disease among others making it the target f
Cell Biolabs Inc OxiSelect™ DNA Double-Strand Break Staining Kit, Trial Size, 20 assays
DNA double-strand breaks (DSBs) are probably the most dangerous of the many different types of DNA damage that occur within the cell. A very early step in the cellular response to DSBs is the phosphorylation of a histone H2A variant, H2AX, at the sites of DNA damage. H2AX is rapidly phosphorylated (within seconds) at serine 139 when DSBs are introduced into mammalian cells resulting in discrete γ-H2AX (phosphorylated H2AX) foci at the DNA damage sites. Phosphorylation of H2A at serine 139 causes chromatin decondensation and appears to play a critical role in the recruitment of repair or damage-signaling factors to the sites of DNA damage. Cell Biolabs’ OxiSelect™ DNA DSB Staining Kit is based on the phosphorylation of the histone H2A.X at serine 139 in response to DNA damaging agents which cause double strand breaks in cells that are cultured in microtiter plates. This Trial Size kit provides sufficient reagents for up to 20 stainings in a 96-well plate.
Dojindo Molecular Technologies Inc Cytotoxicity LDH Assay Kit-WST (500 TESTS) measures released LDH from dead cells and can be used with and without transferring supernatant.
Lactate dehydrogenase(LDH) is an enzyme that is present in almost all cell types and it catalyzes the oxidation of lactate to pyruvate in the presence of co-enzyme NAD+. Once cells are impaired by stress, injuries, chemicals or intercellular signals, LDH is rapidly released from the cell membrane. Thus, the measurement of the amount of released LDH from cells is one of the major methods to assess cell death. Since Dojindo's Cytotoxicity LDH Assay Kit-WST neither reflects the activity of living cells nor is harmful to cells, it allows the assay to perform in wells containing both viable and damaged cells. Cell Cytotoxicity is evaluated by multiple cell functions. Dojindo provides you useful selection guide. (https://www.dojindo.com/cell-proliferation-cytotoxicity-assay-kits-selection-guide/)
Amsbio LLC Sulfated Glycosaminoglycan Quantification Kit (Proteoglycan Detection)
Sulfated Glycosaminoglycan Quantification Kit (Proteoglycan Detection)
Revvity Health Sciences Inc DELFIA EuTDA Cytotoxicity Detection kit, 1 item
DELFIA EuTDA Cytotoxicity Detection kit, 1 item
Cell Signaling Technology BrdU Cell Proliferation Assay Kit 1 Kit
BrdU Cell Proliferation Assay Kit 1 Kit
Meso Scale Discovery Canine ProInflammatory Panel 3 Ultra-Sensitive Kit
This item has a minimum order quantity of 5 packs per supplier requirements. Cytokines & Chemokines , Immunology/Inflammationm, Canine, 96 wells
Meso Scale Discovery KIT PHOS-/TOTAL ERK1/2 5PK
MS2400 Phospho-, Total ERK1/2 Whole Cell Lysate Kit (5 Pl)