General Cell Based Assays
Abcam Acetoacetate Assay Kit (Colorimetric)
Acetoacetate Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical NADP+ (hydrate), Purity: ≥90%, 250 mg
The oxidized form of NADPH; serves as a cofactor in various biological reactions
Eurofins Discoverx PathHunter Human IL-15 (IL-15R (IL2RB/IL2RG)) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human IL-15 (IL-15R (IL2RB/IL2RG)) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of IL2RB/IL2RG. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Enzo Life Sciences FLUOR DE LYS-Green SIRT5 fluorometric drug discovery assay kit (100 tests)
The FLUOR DE LYS Green SIRT5 Fluorometric Drug Discovery Kit is a complete assay system designed to measure the lysyl desuccinylase activity of the recombinant human SIRT5 included in the kit. A black 96-well microplate is packaged with the kit, but it should be noted that reagents of the FLUOR DE LYS system have also been successfully employed in other formats. The Green SIRT5 Fluorescent Activity Assay is based on the unique FLUOR DE LYS-Succinyl GreenSubstrate/Developer combination. The FLUOR DE LYS-Succinyl GreenSubstrate, which comprises a lysine residue, Nε-succinylated on its side-chains, is first incubated with human recombinant SIRT5 together with the cosubstrate NAD+. Desuccinylation of FLUOR DE LYS-Succinyl Green sensitizes it so that, in the second step, treatment with the FLUOR DE LYS Developer produces a fluorophore. Alternative name: Green sirtuin 5 fluorescent assay kit. Applications: Fluorescent detection, HTS Cell-based assays, Activity assay.
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-VASP (Ser157) Detection Kit, 96 Assay Points
HTRF Human & Mouse Phospho-VASP (Ser157) Detection Kit, 96 Assay Points
American Research Products Inc HOXB2 Transcription Factor Activity Assay
HOXB2 Transcription Factor Activity Assay
Abcam Sodium Assay Kit (Colorimetric)
Sodium Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Molecular Devices QBT Fatty Acid Uptake Assay, Bulk, BODIPY-dodecanoic, 96/384-well
The QBT Fatty Acid Uptake Assay Kit is a homogeneous, fluorescence-based assay for measuring live-cell fatty acid uptake kinetics and screening fatty acid transporters in high-throughput formats. The assay uses a fluorescent fatty acid analog with a quench reagent to reduce extracellular background and enable kinetic readings.
- Homogeneous fluorescence assay suitable for high-throughput screening.
- Uses a BODIPY-dodecanoic fluorescent fatty acid analog and quench reagent.
- Bulk kit contains 10 vials, sufficient for approximately 50 plates.
- Compatible with 96-well and 384-well microplate formats.
- Measure kinetics with bottom-read fluorescence, excitation ~470-500 nm, emission ~500-560 nm.
- Store kit at -20°C; formulated loading buffer stable up to 8 hours at room temperature.
Revvity Health Sciences Inc HTRF mAb Anti-Human c-Myc Eu-Conjugate, 5,000 Assay Points
HTRF mAb Anti-Human c-Myc Eu-Conjugate, 5,000 Assay Points
Eurofins Discoverx cAMP Hunter eXpress rDRD1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0128E2CP2L)
The cAMP Hunter rDRD1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine, A68930) based activation of rDRD1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type rDRD1, a naturally Gs coupled GPCR. Upon activation of rDRD1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cell Biolabs Inc OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence), Trial Size, 20 assays
The OxiSelect™ In Vitro ROS/RNS Assay Kit is an assay for measuring the total free radical presence of a sample. The assay employs a proprietary quenched fluorogenic probe, DCFH-DiOxyQ), which is based on similar chemistry to the popular DCFH-DA. The DCFH-DiOxyQ probe is first primed with a quench removal reagent, and subsequently stabilized in the highly reactive DCFH form. In this reactive state, ROS and RNS species can react with DCFH, which is rapidly oxidized to the highly fluorescent 2’, 7’-dichlorodihydrofluorescein (DCF) . The DCFH-DiOxyQ probe can react with hydrogen peroxide, peroxyl radical, nitric oxide, and peroxynitrite anion. These free radical molecules are representative of both ROS and RNS, thus allowing for measurement of the total free radical population within a sample. The kit has a detection sensitivity limit of 40 nM for H2O2. Each trial size kit provides sufficient reagents to perform up to 20 assays, including standard curve and unknown samples.
Abcam Cholesterol Assay Kit (Cell-Based)
Cholesterol Assay Kit (Cell-Based)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress mCCR9 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0734E2MCP2L)
The PathHunter mCCR9 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL25) based activation of mCCR9 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR9 and EA-tagged β-Arrestin-2. Activation of mCCR9 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress CHRM2 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0078E2CP2M)
The cAMP Hunter CHRM2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. oxotremorine) based activation of CHRM2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CHRM2, a naturally Gi/Go coupled GPCR. Upon activation of CHRM2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ILEX LIFE SCIENCES LLC QuickZyme Hydroxyproline Assay Kit Colorimetric Method 1/EA
QuickZyme Hydroxyproline Assay Kit 2 x 96 Assays (includes 2x 96-well plates) The QuickZyme Hydroxyproline Assay Kit enables quantitative collagen analysis in all types of tissues in a fast and simple 96-well plate format This colorimetric assay does not need the drying step following acid hydrolysis of collagen for which often special equipment is needed This assay is the ONLY hydroxyproline-based assay that does not need this laborious step resulting in a fast sensitive and easy assay NOTE This kit does not contain tubes or a protocol for collagen hydrolysis If you need these items please order the QuickZyme Total Collagen Assay Application conditioned media cell extracts tissues tissue extracts Sensitivity 2 4 M (0 3 g/ml) Assay-time 2 hours QuickZyme Hydroxyproline Assay can be paired with QuickZyme Total Protein Assay to allow the expression of data as hydroxyproline quantity per total amount of protein