General Cell Based Assays
Abcam WST-1 ASSAY REAGENT - CELL PRO ..
WST-1 ASSAY REAGENT - CELL PRO ..
The product is subject to the following: Abcam Restricted Use Statement
Dojindo Molecular Technologies Inc Liperfluo(50UGx5) is used to monitor lipid peroxidation in ferroptosis research, emitting intense fluorescence by a lipid peroxide specific oxidation, and is applicable for microscopy and FCM analysis
Liperfluo is designed and exclusively developed by Dojindo for detection of lipid peroxides. Among fluorescent probes that detect Reactive Oxygen Species (ROS), Liperfluo is the only compound that can specifically detect lipid peroxides. Since the excitation and emission wavelengths of the oxidized Liperfluo are 524 nm and 535 nm, respectively, both photo-damage and auto-fluorescence can be minimized. The tetraethyleneglycol group linked to one end of the diisoquinoline ring helps its solubility and dispersibility to aqueous buffer. Though Liperfluo's oxidized form is almost nonfluorescent in an aqueous media, it emits fluorescence in lipophilic sites such as cell membranes. Therefore, it can easily be applied to lipid peroxide imaging by a fluorescence microscopy and a flow cytometric analysis for living cells. Liperfluo is widely used in ferroptosis research, a research field Dojindo has a strong portfolio in. (https://www.dojindo.com/ferroptosis-detection-selection-guide/)
Eurofins Discoverx PathHunter eXpress TGFBR1/ACVR2 Dimerization Assay, 10pl Kit. (93-1012E3CP5L)
The PathHunter TGFBR1/ACVR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GDF-11) induced dimerization of TGFBR1/ACVR2 receptors. This product uses EFC technology. The cell line included overexpresses TGFBR1 target protein fused to PK alongside ACVR2 target protein fused to EA. Upon ligand-induced dimerization of TGFBR1/ACVR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human DPPIV ASY
The Meso Scale Discovery U-PLEX Human DPP4 Assay is a multiplex immunoassay panel that measures Dipeptidyl Peptidase 4 (DPP4) in human serum plasma and cell culture supernatants This assay utilizes MSD s electrochemiluminescent detection technology and is designed for use on MSD s QuickPlex SQ 120 instrument The assay has high sensitivity and wide dynamic range It requires a low sample volume of 50 L The total assay time is approximately 3 5 hours This product provides a fast simple and robust way to measure DPP4 levels across multiple samples
Eurofins Discoverx AssayComplete Cell Plating 3 Reagent, 500. (93-0563R3B)
AssayComplete Cell Plating 3 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Cayman Chemical COX Activity Assay Kit, 96 Well
Cayman’s COX Activity Assay Kit measures the peroxidase activity of COX. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N’,N’-tetramethyl-p-phenylenediamine (TMPD) at 590 nm.{8581} It can be used with tissue homogenates and purified enzyme preparations. The kit includes isozyme-specific inhibitors for distinguishing COX-2 activity from COX-1 activity.
Eurofins Discoverx PathHunter Human AR Nuclear Translocation Cell-Based Assay Kit (HEK 293)
The PathHunter Human AR Stable Cell Line Nuclear Translocation Assay (HEK 293) contains a stable clonal cell line used to measure AR nuclear hormone receptor activation via nuclear translocation. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Abcam Autophagy Assay Kit
Autophagy Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
STEMCELL Technologies DiOC63 Iodide
Green fluorescent lipophilic dye for staining cell membranes and other hydrophobic structures in live and fixed cells - 25 Mg
Bioassay Systems QuantiChrom™ Hydroxyproline Assay Kit. For quantitative determination of hydroxyproline and collagen in biologic and cosmetic samples.
For quantitative determination of hydroxyproline and collagen in biologic and cosmetic samples. Key Features: Safe. Our hydroxyproline assay uses an improved perchlorate-free chemistry. Sensitive and accurate. Uses 20 µL sample. Linear detection range 0.5 µg/mL to 50 µg/mL hydroxyproline in 96-well plate assay. Convenient. Collagen can be hydrolyzed in Eppendorf tubes, and final incubation can be performed at 37°C. Method: OD560nm. Samples: Hydroxyproline and collagen in biologic and cosmetic samples. Species: All. Procedure: Assay takes Approx. 2 hrs. Kit size: 100 Tests. Detection limit: 0.5 µg/mL.
Abcam Cholesterol Efflux Assay Kit (Cell-based)
Cholesterol Efflux Assay Kit (Cell-based)
The product is subject to the following: Abcam Restricted Use Statement
Abcam ADP Assay Kit (Colorimetric/Fluorometric)
ADP Assay Kit (Colorimetric/Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx InCELL Hunter eXpress PIM1 Kinase Binding Assay, 10pl Kit. (96-0052E15CP0L)
The InCELL Hunter eXpress PIM1 Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of PIM1 via detection of PIM1 levels. This product uses EFC technology. The cell line included overexpresses PIM1 target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CCR1 Activated GPCR Internalization Assay, 10pl Kit. (93-0651E3CP5L)
The PathHunter CCR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CCR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CCR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CCR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress CMKLR1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0080E2CP2M)
The cAMP Hunter CMKLR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. chemerin) based activation of CMKLR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CMKLR1, a naturally Gi/Go coupled GPCR. Upon activation of CMKLR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.