General Cell Based Assays
Enzo Life Sciences FLUOR DE LYS HDAC8 fluorometric drug discovery kit (1x96wells)
A FLUOR DE LYS fluorescent assay system. The HDAC8 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC8 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The FLUOR DE LYS HDAC8 assay is based on the FLUOR DE LYS Substrate and FLUOR DE LYS Developer combination. The assay procedure has two steps. First, the FLUOR DE LYS Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYS Developer produces a fluorophore. Alternative name: Histone deacetylase 8 fluorescent assay kit. Applications: Fluorescent detection, HTS Cell-based assays, Activity assay.
American Research Products Inc VITAMIN E VE COLORIMETRIC KIT
5000265833 VITAMIN E VE COLORIMETRIC KIT
Eagle Biosciences Inc Nitric Oxide Synthase Non-Enzymatic Assay Kit
Nitric Oxide Synthase Non-Enzymatic Assay Kit
Enzo Life Sciences Catalase fluorometric detection kit (500tests)
The Catalase fluorometric detection kit provides a higher throughput sensitive method for measuring catalase activity in just one hour. This is a homogeneous mix-and-read assay that enables you to monitor multiple time points for a kinetic read. Positive control is provided. Sensitivity: (range 0- 4 U/ml). UniProt ID: P00432. Applications: Fluorescent detection, HTS . Species reactivity: Species independent . Content: Reaction buffer concentrate, Detection Reagent, Horseradish peroxidase concentrate, Hydrogen peroxide, Catalase enzyme.
Epigentek Inc Epigenase m6A Methylase Activity/Inhibition Assay Kit (Colorimetric) (48 Assays)
For measurement of total activity/inhibition of m6A methylases from nuclear extracts or purified enzymes
American Research Products Inc TOTAL BILIRUBIN TBIL COLORIMET
5000265066 TOTAL BILIRUBIN TBIL COLORIMET
Cayman Chemical LDL Uptake CellBased Assay Ki
LDL uptake and its regulation are important therapeutic targets for atherosclerosis and related diseases. Cayman Chemical’s LDL Uptake Cell-Based Assay employs a preparation of human LDL conjugated to Dylight™ 550 as a fluorescent probe for detection of LDL uptake into cultured cells. A LDL receptor-specific antibody and a Dylight™ 488-conjugated secondary antibody are included in the kit for identifying the distribution of LDL receptors. The kit provides a convenient tool for studying LDL uptake and its regulation at the cellular level.
Cayman Chemical Acid Phosphatase Colorimetric
Cayman’s Acid Phosphatase Assay provides a convenient method for detecting AP activity in plasma, serum, urine, and semen. The assay utilizes para-nitrophenyl phosphate (pNPP) as a chromogenic substrate for the enzyme. In the first step, AP dephosphorylates pNPP. In the second step, the phenolic OH-group is deprotonated under alkaline conditions resulting in p-nitrophenolate that yields an intense yellow color which can be measured at 405-414 nm. The kit provides all reagents needed to assay AP activity, and includes L-tartrate, an inhibitor of non-tartrate resistant APs.
Cayman Chemical 20S Proteasome Assay Kit
The 20S proteasome is the proteolytic core of a large protein degradation complex, the 26S proteasome. Proteasome inhibitors exhibit anti-inflammatory and antiproliferative effects, evidence that the proteasome may be an important drug target for the treatment for cancer and inflammatory diseases. Cayman’s 20S Proteasome Assay employs a specific 20S substrate, SUC-LLVY-AMC which, upon cleavage by the active enzyme, generates a highly fluorescent product with an emission wavelength at 480 nm.
Enzo Life Sciences CV-Cathepsin L detection kit (1 Kit)
Elevated cathepsin enzyme activity often indicates the presence of a pathological condition including cancer, Alzheimer's disease, autoimmune disease, and osteoporosis. Based on the dye cresyl violet and the cathepsin L PheArg peptide recognition sequence, this kit allows the detection of cathepsin L activity in living cells by fluorescence microscopy. Once cells are grown, staining is completed in just a few minutes. Hoechst 33342 stain and Acridine Orange are also included to allow visualization of nuclei and lysosomes. Applications: Fluorescence microscopy, Fluorescent detection .