General Cell Based Assays
Eurofins Discoverx PathHunter eXpress GPR6 U2OS β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0634E3CP1L)
The PathHunter GPR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR6 and EA-tagged β-Arrestin-2. Activation of GPR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical LDL Uptake Flow Cytometry Assay Kit, 48 test
Cayman’s LDL Uptake Flow Cytometry Assay Kit employs human LDL conjugated to DyLight™ 488 as a convenient tool for studying the uptake of LDL in cultured cells. Flow cytometry provides the advantage of assessing the uptake of LDL at the single-cell level. In addition, multiplexing with other markers, such as LDLR expression, is possible to gain more information from a single experiment. Lovastatin is included in this kit as a control modulator of LDL uptake by hepatocytes. The reagents provided in this kit are sufficient to test 48 samples by FC.
Eurofins Discoverx PathHunter eXpress HEK 293 RELA Nuclear Translocation Assay, 10-plate Kit. (93-0907E1CP0L)
The PathHunter eXpress HEK 293 RELA Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the NFκB pathway. This assay kit enables assessment of ligand (e.g. IL-1α) based pathway activation via detection of resulting RELA nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLink-tagged RELA and nuclear localized EA. Upon pathway activation, induced nuclear translocation of RELA leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR78 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0370E2ACP1M)
The PathHunter GPR78 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR78 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR78 and EA-tagged β-Arrestin-2. Activation of GPR78 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical NAD/NADH Cell-Based Assay Kit, 1 ea
Nicotinamide adenine dinucleotide (NAD) exists in an oxidized form, NAD+, as well as a reduced form, NADH. NAD, the main free form in cells, functions in modulating cellular redox status and by controlling signaling and transcriptional events, making it an important cofactor when investigating normal cellular function. Cayman’s NAD/NADH Cell-Based Assay Kit provides a colorimetric ethod for measuring intracellular NAD+and NADH in cultured cells. In this assay, NAD+ found in cell samples is reduced to NADH by alcohol dehydrogenase during the oxidation of ethanol to acetaldehyde. The newly formed and the existing NADH found in the samples is then oxidized resulting in the reduction of a tetrazolium salt substrate (WST-1) to a highly-colored formazan which absorbs at 450 nm. The amount of formazan produced is proportional to the amount of total NAD in the cell lysate and can be used as an indicator of the total cellular NAD concentration.
Eurofins Discoverx PathHunter eXpress IL17RD/TNFR2 Dimerization Assay, 10pl Kit. (93-1068E3CP5L)
The PathHunter IL17RD/TNFR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. TNFα) induced dimerization of IL17RD/TNFR2 receptors. This product uses EFC technology. The cell line included overexpresses IL17RD target protein fused to PK alongside TNFR2 target protein fused to EA. Upon ligand-induced dimerization of IL17RD/TNFR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress rPROKR2 CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0482E2RCP0L)
The PathHunter rPROKR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prokineticin) based activation of rPROKR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rPROKR2 and EA-tagged β-Arrestin-2. Activation of rPROKR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Xanthine/Hypoxanthine Assay Kit
Xanthine/Hypoxanthine Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Abcam Maleimide Quantification Assay Kit, 200 tests per kit
Maleimide Quantification Assay Kit, 200 tests per kit
The product is subject to the following: Abcam Restricted Use Statement
Aat Bioquest iFluor™ 350 succinimidyl ester
AAT Bioquest's iFluor™ dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins.
Aat Bioquest Amplite™ Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*
The glucose oxidase is a dimeric protein that catalyzes the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid.
Eurofins Discoverx PathHunter eXpress DRD2L (Long Isoform) CHO-K1 β-Arrestin GPCR Assay, 2pl Kit. (93-0579E2CP2M)
The PathHunter DRD2L eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD2L GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DRD2L and EA-tagged β-Arrestin-2. Activation of DRD2L stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Aat Bioquest Amplite™ Fluorimetric NAD Assay Kit *Blue Fluorescence*
Nicotinamide adenine dinucleotide (NADH) and its oxidized form (NAD) are essential cofactors for many enzyme reactions found in living cells.
Eurofins Discoverx PathHunter eXpress GPR148 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0364E2ACP1M)
The PathHunter GPR148 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR148 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR148 and EA-tagged β-Arrestin-2. Activation of GPR148 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.