General Cell Based Assays
Eurofins Discoverx AssayComplete Cell Plating 13 Reagent, 100. (93-0563R13A)
AssayComplete Cell Plating 13 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress c-Ret-GFRα1 Functional Assay, 2-Plate Kit. (93-1054E3CP16M)
The PathHunter eXpress c-Ret-GFRα1 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated c-Ret and GFRα1 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged c-Ret and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, GFRα1 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the cynomolgus version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress cyno CXCR2 HEK 293 β-Arrestin GPCR Assay, 10pl Kit. (93-0981E1CCP0L)
The PathHunter cyno CXCR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL8/IL8) based activation of cyno CXCR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged cyno CXCR2 and EA-tagged β-Arrestin-2. Activation of cyno CXCR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Cynomolgus monkey version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mPTGER4 DLD1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0982E13MCP25M)
The PathHunter mPTGER4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of mPTGER4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mPTGER4 and EA-tagged β-Arrestin-2. Activation of mPTGER4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress PTGER3 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0159E2CP2L)
The cAMP Hunter PTGER3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2, cloprostenol) based activation of PTGER3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTGER3, a naturally Gi/Go coupled GPCR. Upon activation of PTGER3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human C1q receptor ASY
The Meso Scale Discovery R-PLEX Human C1q Receptor Assay is a multiplex immunoassay panel that measures C1q Receptor in human serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5-logs of dynamic range and low sample volume requirements The assay is available as a singleplex or as a multiplex combining up to 10 biomarkers per well It requires 50 L of sample and can perform up to 80 tests across two plates The assay takes 3 5 hours to complete and has a typical sensitivity of 19 pg/mL
Eurofins Discoverx PathHunter Human c-Kit Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human c-Kit Stable Cell Line Functional Kinase Assay (U2OS) measures c-KIT receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human IL-27 (IL27RA/IL6ST) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human IL-27 (IL27RA/IL6ST) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of IL27RA/IL6ST. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human PROKR1 Total GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human PROKR1 Stable Cell Line Total GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure PROKR1 (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Abcam Cystathionine beta Synthase Assay Kit
Cystathionine beta Synthase Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter Human HRH2 (H2) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human HRH2 (H2) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure HRH2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery R-PLEX Human CD5L ASY
The R-PLEX Human CD5L Assay from Meso Scale Diagnostics is a multiplex immunoassay kit that enables the simultaneous quantitative measurement of CD5L in human serum plasma and cell culture supernatants This assay utilizes MSD s electrochemiluminescent detection technology and is provided in a convenient 96-well plate format The kit includes a CD5L-specific antibody coated plate detection antibody calibrator and assay diluent The assay has a typical sensitivity of 0 3 ng/mL and an upper limit of quantification of 2500 ng/mL for CD5L This product can aid in the quantification of CD5L for life science research applications
Dojindo Molecular Technologies Inc Cellular Senescence Plate Assay Kit (100TESTS), easily quantify SA-β-gal activity via plate reader, suitable for multiple sample assays
This product is a simple detection kit by plate assay for senescence-associated beta-galactosidase (SA-β-gal) activity which is used as a marker for senescent cells. By simply adding SPiDER-Gal, a reagent for detection of β-galactosidase, to 96 well plates, this kit allows you to quantify SA-β-gal activity and makes it possible to evaluate multiple specimens. When normalization is done by the results obtained by counting cells, quantifying nucleic acids, or quantifying proteins, the measured values obtained using this kit become available for evaluating SA-β-gal activity according to cell number. The features of cellular senescence were summarized based on modern publications that have a high number of citations on this page (https://www.dojindo.com/senescent-cell-detection-selection-guide).
Eurofins Discoverx PathHunter Human RXFP3 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human RXFP3 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure RXFP3 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.