General Cell Based Assays
Eurofins Discoverx PathHunter Human P2RY2 β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human P2RY2 β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure P2RY2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter™ Human SSTR4 Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human SSTR4 Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure SSTR4 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GHR-JAK1 Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human GHR-JAK1 Stable Cell Line Functional Kinase Assay (U2OS) measures GHR cytokine receptor activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human OPRM1 (Opioid mu) β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human OPRM1 (Opioid mu) β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure OPRM1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery U-PLEX Mouse IL-6 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-6 Assay Kit is a sensitive immunoassay for detecting mouse interleukin 6 (IL-6) in various samples It uses MSD s electrochemiluminescent technology offering high sensitivity a wide dynamic range and quick results The kit includes a 96-well plate with capture antibodies for mouse IL-6 detection antibodies with an electrochemiluminescent label and calibrators for a standard curve Sample incubation is 2 hours with no wash steps quantifying IL-6 from 0 2-10 000 pg/mL
Meso Scale Discovery R-PLEX Human cIAP1 ASY
The R-Plex Human CIAP1 Assay from Meso Scale Diagnostics is a multiplex immunoassay that measures the levels of Cellular Inhibitor of Apoptosis Protein 1 (CIAP1) in human serum plasma and cell culture supernatants This assay utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements The assay can monitor CIAP1 levels across four orders of magnitude from sub-picogram to nanogram levels per mL This quantitative immunoassay allows the study of CIAP1 levels in biological samples for life science research
Eurofins Discoverx PathHunter Human FPR1 Total GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human FPR1 Stable Cell Line Total GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure FPR1 (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human PYY (NPY2R) Activated GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human PYY (NPY2R) Stable Cell Line Activated GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure NPY2R (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter™ Human MTNR1A Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human MTNR1A Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure MTNR1A (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter eXpress GPR35 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0152E2CP2M)
The cAMP Hunter GPR35 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. zaprinast) based activation of GPR35 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GPR35, a naturally Gi/Go coupled GPCR. Upon activation of GPR35, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Sigma Aldrich Fine Chemicals Biosciences Superoxide Dismutase SOD A
Superoxide dismutase (SOD) is part of a family of metal ion-containing enzymes (SODs) that are found in most organisms from bacteria to mammals. SODs catalyze the dismutation of superoxide (generated by aerobic respiration) to molecular oxygen and peroxide. Superoxide dismutase form the front line of defence against reactive oxygen species (ROS)-mediated injury and harbor anti-inflammatory activities. The Superoxide Dismutase (SOD) Activity Assay Kit provides a simple and sensitive procedure for measuring SOD enzymatic activity in various sample types. SOD activity is determined by measuring the decrease in superoxide anions (generated by the enzyme xanthine oxidase [XO]). Since superoxide anions interact with the provided WST dye yielding color at 450 nm the decrease in the color signal is proportional to SOD inhibition activity. The kit allows the determination of SOD activity in percent inhibition or in units of activity using the provided SOD Enzyme as a standard. The provided
LICORbio GAR CellTag™ 700 Stain ICW Kit II
GAR CellTag 700 Stain ICW Kits provide detection reagents for cell-based In-Cell Western™ Assays. This cost-effective normalization method makes quantification of the target protein more precise. CellTag 700 Stain accumulates in the nucleus and cytoplasm of permeabilized cells allowing for accurate normalization to cell number. This kit includes reagents to perform an In-Cell Western assay and CellTag 700 Stain to normalize well-to-well variations in cell number. CellTag 700 accumulates in the nucleus and cytoplasm of permeabilized cells allowing for accurate normalization to cell number. Kit Components include IRDye 800CW Goat anti-Rabbit Secondary Antibody (0.5 mg), Intercept (TBS) Blocking Buffer (1 x 500 mL), CellTag 700 Stain (2 x 10 nmol), and Kit Protocol
Eurofins Discoverx PathHunter eXpress HCRTR1 Total GPCR Internalization Assay, 10pl Kit. (93-0729E3CP0L)
The PathHunter HCRTR1 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of HCRTR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged HCRTR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the HCRTR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human & Mouse Total GSK3β Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Total GSK3β Detection Kit, 50,000 Assay Points
Abcam Acetylcholinesterase Assay Kit (Fluorometric Red)
Acetylcholinesterase Assay Kit (Fluorometric Red)
The product is subject to the following: Abcam Restricted Use Statement