General Cell Based Assays
Eurofins Discoverx InCELL Hunter eXpress RIPK2 Kinase Binding Assay, 10pl Kit. (96-0050E1CP0L)
The InCELL Hunter eXpress RIPK2 Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of RIPK2 via detection of RIPK2 levels. This product uses EFC technology. The cell line included overexpresses RIPK2 target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TACR3 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0501E2CP2M)
The PathHunter TACR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. substance P, senkitide) based activation of TACR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged TACR3 and EA-tagged β-Arrestin-2. Activation of TACR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CCR4 Total Internalization Assay, 10pl Kit. (93-0845E3CP27L)
The PathHunter CCR4 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CCR4 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged CCR4 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CCR4 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human FGFR1v3B Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human FGFR1v3B Stable Cell Line Functional Kinase Assay (U2OS) measures FGFR1 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GRPR Activated GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human GRPR Stable Cell Line Activated GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure GRPR (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human ADGRB2 (BAI2) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human ADGRB2 (BAI2) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure BAI2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human LEPR/LEPR Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human LEPR/LEPR Dimerization Stable Cell Line Assay (U2OS) measures ligand-induced dimerization of LEPR/LEPR. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cayman Chemical THUNDRPhosphop38a MAPK 4800Wel
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p38αβγ MAPK14/11/12 (T180/Y182) in cell lysates
Eurofins Discoverx PathHunter eXpress CHRM3 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0860E3CP0L)
The PathHunter CHRM3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. acetylcholine) based activation of CHRM3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM3 and EA-tagged β-Arrestin-2. Activation of CHRM3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Mouse IL-33 ASY
The Meso Scale Discovery U-PLEX Mouse IL-33 Assay Kit is a multiplex immunoassay that measures mouse interleukin 33 (IL-33) in serum plasma cell culture supernatants and other biological fluids This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements The kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with MSD SULFO-TAG calibrators and an MSD read buffer Sample incubation time is just 2 hours The kit can test up to 80 samples in duplicate and has a lower limit of detection of 0 04 pg/mL for mouse IL-33
Eurofins Discoverx PathHunter Human S1P2 (EDG5) Activated GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human S1P2 (EDG5) Stable Cell Line Activated GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure EDG5 (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GPR45 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR45 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR45 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GPR162 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR162 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR162 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Sigma Aldrich Fine Chemicals Biosciences Oxalate Assay Kit sufficie
Oxalate or Oxalic Acid is a metabolic breakdown product of the Krebs Cycle in eukaryotes and the glyoxylate cycle in other microorganisms. It can be found in the urine of humans and other mammals. Oxalate concentration can be used as a measure of kidney function where a high level of oxalate is an indicator for kidney stones which are primarily made of the insoluble salt calcium oxalate. Measuring oxalate is more accurate than measuring calcium as a marker for kidney stones because calcium is excreted at high concentrations even in normal urine.