General Cell Based Assays
Cytek Biosciences Inc Oxidative Stress Kit Amnis
Product Name: Muse Oxidative Stress Kit
Cytek Catalog Number: MCH100111
Product Class: Guava Muse Reagent
Type: Muse
Size: 100 Tests
Sino Biological SMS 293-SUPI Expression Medium Supplement 100 mL
SMS 293-SUPI is a protein-free, serum-free liquid supplement that is specifically used to meet the nutritional requirements of HEK293(such as 293-F, 293H, etc.) suspension cells at high-density growth and late transient transfection. This supplement contains high concentrations of essential nutrients, such as amino acids and inorganic salts, required for cell growth?without L-Glutamine.
Cayman Chemical NADP+ (hydrate), Purity: ≥90%, 100 mg
The oxidized form of NADPH; serves as a cofactor in various biological reactions
Cayman Chemical NADP+ (hydrate), Purity: ≥90%, 1 g
The oxidized form of NADPH; serves as a cofactor in various biological reactions
BioVendor, LLC QuickZyme Sensitive Tissue Hydroxyproline Kit 2 X 96-Assays (for collagen analysis)
QuickZyme Sensitive Tissue Hydroxyproline Kit, 2 X 96-Assays. Measure hydroxyproline in small tissue samples with low collagen levels! The ONLY hydroxyproline-based assay which shows no matrix effect and no need for further dilution of the hydrolysate, enabling measurement in small samples with low hydroxyproline levels. No drying of hydrolysate is needed, resulting in a fast (<2 hrs), sensitive and easy (96-well plate format) assay. Dynamic range: 6-300 uM. Sensitivity: 0.3 ug/ml. Species independent. Measures total hydroxyproline. If this hydroxyproline is obtained upon hydrolysis of collagen, it represents all collagen types present without discriminating between types of collagen, procollagen, mature collagen and collagen degradation products. Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present. Collagen hydrolysis tubes & protocol not included (if these items are needed, please order QuickZyme Sensitive Tissue Collagen Assay).
Dojindo Molecular Technologies Inc Cellular Senescence Detection Kit (1 plate), detects SA-β-gal with high sensitivity, applicable to both living and fixed cells, for cell sample by fluorescence microscopy or flow cytometry.
SPiDER-βGal is a new reagent to detect β-galactosidase specifically which possesses a high cell-permeability and a high retentivity inside cells. This novel reagent can detect β-galactosidase not only in fixed cells but also in living cells, and also can be applied to quantitative analysis by flow cytometry. Senescence-associated beta-galactosidase (SA-β-gal), which is overexpressed in senescent cells, has been widely used as a marker of cellular senescence. Cellular Senescence Detection Kit – SPiDER-βGal is optimized to detect intracellular SA-β-gal. Modern publications with a high number of citations summarize the various features of cellular senescence on the following page (https://www.dojindo.com/senescent-cell-detection-selection-guide/).
Eurofins Discoverx AssayComplete Cell Culture Kit-117, (92-3117G)
AssayComplete Cell Culture Kit-117 is an optimized media kit intended for use in culturing and propagating Eurofins DiscoverX cell lines. This kit contains the base medium, relevant serum, and pen/strep required to produce a full cell culture medium for optimal cell culture results. This product is provided as a 3-component kit which will produce approximately 550 mL of finished media. Please refer to cell line specific datasheet to determine the recommended cell culture kit. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter Detection Kit, 15 (2 plates), 200/800 dp (96-well/384-well). (93-0001M)
The PathHunter Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter cell lines or with custom cell lines made using ProLabel/PK expression vectors. This kit contains lysis buffer and EFC substrate to allow for detection of EFC levels in the assayed cells. This kit contains all components required to assay 2 plates (96/384 well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-0001m.
Eurofins Discoverx cAMP Hunter eXpress GLP1R CHO-K1 GPCR Assay, 2-Plate Kit. (95-0062E2CP2M)
The cAMP Hunter GLP1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of GLP1R GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP1R, a naturally Gs coupled GPCR. Upon activation of GLP1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Sartorius IncuCyte pHrodo Red E. coli Bioparticles for Phagocytosis
One vial of 2 mg sterile lyophilized solid sufficient for 100-200 live-cell imaging phagocytosis tests in 96-well format
Cayman Chemical TGR5 (GP-BAR1) Reporter Assay Kit, 1 ea
Cayman's TGR5 (GP-BAR1) Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for expressing TGR5 and a cAMP response element regulated secreted alkaline phosphatase (SEAP) reporter (TGR5 Reverse Transfection Strip Plate).; Cells grown on the transfection complex will express TGR5 at the cell surface within 24 hours.; Binding of agonists to TGR5 initiates a signal transduction cascade through the GαS and adenylate cyclase pathway resulting in the expression of SEAP which is secreted into the cell culture medium.; Aliquots of culture medium are collected at 6-8 hours after stimulation, and SEAP activity is measured following the addition of a luminescence-based alkaline phosphatase substrate provided in the kit.; The kit provides sufficient reagent to measure SEAP activity at three time points using the three included white assay plates.