General Cell Based Assays
Enzo Life Sciences DAR-4M AM (cell permeable) (1mg)
Cell permeable analog of DAR-4M (ALX-620-067). Can be used to detect nitric oxide (NO) directly in cell specimens prepared from various sources, such as the brain (Ex: ~560nm; Em: 575nm). Stable in a pH range of 4-12. Low decrease in fluorescence intensity over time. Detection limit 7nM. Not for sale in Japan. Alternative name: Diaminorhodamine-4M AM. Formulation: Dissolved in 0.32ml DMSO. Purity: ≥98% (HPLC). Long Term Storage: -20°C.
Cayman Chemical Thiol Detection Assay Kit, 96 Well
The detection and measurement of free thiols (i.e., free cysteine, glutathione, and cysteine residues on proteins) is one of the essential tasks for investigating biological processes and events in many biological systems. Cayman’s Thiol Detection provides a simple, reproducible, and sensitive tool for assaying free thiol content in samples (i.e., plasma, serum, tissue homogenates, cell lysates, and urine). The assay utilizes a proprietary fluorometric detector that reacts with thiol groups to emit a strong fluorescent signal which can be detected using excitation wavelengths between 380-390 nm and emission wavelengths between 510-520 nm.
Eurofins Discoverx PathHunter Human LXR alpha Nuclear Receptor Protein Interaction Cell-Based Assay Kit (CHO-K1)
The PathHunter Human LXR alpha Nuclear Receptor Stable Cell Line Protein Interaction Assay (CHO-K1) contains a stable clonal cell line used to measure activated LXRα nuclear hormone receptor interaction with SRCPs. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human RXR gamma Nuclear Receptor Protein Interaction Cell-Based Assay Kit (CHO-K1)
The PathHunter Human RXR gamma Nuclear Receptor Stable Cell Line Protein Interaction Assay (CHO-K1) contains a stable clonal cell line used to measure activated RXRγ nuclear hormone receptor interaction with SRCPs. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Abcam Cathepsin B Assay Kit
Cathepsin B Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx AssayComplete Cell Plating 36 Reagent, 100. (93-0563R36A)
AssayComplete Cell Plating 36 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress GLP2R CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0572E2CP2L)
The PathHunter GLP2R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GLP-2) based activation of GLP2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP2R and EA-tagged β-Arrestin-2. Activation of GLP2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress AGTRL1 CHO-K1 β-Arrestin-1 GPCR Assay, 10pl Kit. (93-1050E2CP2L)
The PathHunter AGTRL1 (ARR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. apelin-13) based activation of AGTRL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AGTRL1 and EA-tagged β-Arrestin-1. Activation of AGTRL1 stimulates the recruitment of β-Arrestin-1 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical LipidLaunch™ LNP-0315 Uptake Kit (Green Fluorescence), 1 ea
Cayman’s LipidLaunch™ LNP-0315 Uptake Kit (Green Fluorescence) is intended to serve as a starting point for laboratories to explore the uptake of LNPs by their target cell type without the need for specialized equipment. Optimal preparation conditions for the encapsulation of nucleic acids with LNPs must be determined by the end user.
Abcam Wound Healing Assay
Wound Healing Assay
The product is subject to the following: Abcam Restricted Use Statement
ABclonal Technology Cathepsin B Rabbit pAb
This gene encodes a member of the C1 family of peptidases. Alternative splicing results in multiple transcript variants, with at least one encoding a preproprotein that is processed into cathepsin B light and heavy chains, which dimerize to form the active enzyme. Cathepsin B is a lysosomal cysteine protease with endopeptidase and exopeptidase activity, involved in protein turnover. It also acts as amyloid precursor protein secretase, with incomplete APP processing linked to Alzheimers disease. Overexpression of this protein is associated with esophageal adenocarcinoma and other cancers. Both cathepsin B and L are involved in cleaving the spike protein of SARS-CoV-2 during cell entry. Multiple pseudogenes of this gene have been identified.
Abcam Lovastatin (Mevinolin), HMG-CoA reductase inhibitor, 50MG
MW 404.5 Da, Purity >98%. Potent, competitive HMG-CoA reductase inhibitor (Ki = 0.6 nM). Blood-brain barrier permeable. Inhibits in vivo and in vitro cholesterol biosynthesis. Also reduces α-synuclein levels in vitro and in vivo.
The product is subject to the following: Abcam Restricted Use Statement
Cell Biolabs Inc CytoSelect™ 24-Well Cell Migration Assay Kit (3 µm, Fluorometric), 12 assays
Chemotaxis, a type of cell migration, is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia that are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions. The CytoSelect™ Cell Migration Assay Kit utilizes polycarbonate membrane inserts (8 µm pore size) to assay the migratory properties of cells. The kit contains twelve 3 um polycarbonate membrane inserts in a 24-well plate, through which migratory cells are able to pass. Cells are quantified using the provided fluorometric dye. The 3 µm pore size is optimal for measuring the migration of leukocytes.
Abcam BC 11 hydrobromide, Urokinase (uPA) inhibitor, 50MG
MW 290.97 g/mol, Purity >99%. Selective urokinase (uPA) inhibitor (IC₅₀ = 8.2 μM). Shows selectivity over 8 other related enzymes.
The product is subject to the following: Abcam Restricted Use Statement
Meso Scale Discovery U-PLEX Mouse IL-4 ASY
The Meso Scale Discovery U-PLEX Mouse IL-4 Assay Kit is a sensitive immunoassay for detecting and quantifying mouse interleukin 4 (IL-4) in samples like serum plasma and cell culture supernatant It uses MSD s electrochemiluminescent technology offering high sensitivity a wide dynamic range and quick results The assay detects IL-4 at 0 03 pg/mL with a 7-log range The kit includes a 96-well plate with capture antibodies detection antibodies and calibrators The assay completes in 3 5 hours after a 2-hour incubation providing a robust tool for research