General Cell Based Assays
Meso Scale Discovery R-PLEX Mouse Pentraxin 3 ASY
The Meso Scale Discovery R-PLEX Mouse Pentraxin 3 Assay is a multiplex immunoassay that quantitatively measures mouse Pentraxin 3 in serum plasma and cell culture supernatants This assay utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision The assay requires 50 L or less sample volume and can perform up to 80 tests across two plates The assay has a lower limit of detection of 0 04 ng/mL and an upper limit of 10 000 ng/mL This assay allows for the rapid reproducible quantification of mouse Pentraxin 3 from various biological matrices
Eurofins Discoverx PathHunter eXpress TGFBR1/TGFBR2 Dimerization Assay, 10-plate Kit. (93-0889E3CP5L)
The PathHunter TGFBR1/TGFBR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. TGF-β1) induced dimerization of TGFBR1/TGFBR2 receptors. This product uses EFC technology. The cell line included overexpresses TGFBR1 target protein fused to PK alongside TGFBR2 target protein fused to EA. Upon ligand-induced dimerization of TGFBR1/TGFBR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Montana Molecular Red Upward cADDis cAMP sensor Big Sky BacMam 5mL
Detect cAMP signaling kinetics in living cells with the Red Upward cADDis fluorescent cAMP assay. Increase in fluorescence indicates increase in cAMP. Packaged in Big Sky BacMam, for enhanced expression in HEKs, CHOs, and many other mammalian cell types and iPSCs. This product is an updated version of U0200R, with improved expression efficiency from Big Sky BacMam.
Eurofins Discoverx PathHunter Human PPAR gamma Nuclear Receptor Protein Interaction Cell-Based Assay Kit (CHO-K1)
The PathHunter Human PPAR gamma Nuclear Receptor Stable Cell Line Protein Interaction Assay (CHO-K1) contains a stable clonal cell line used to measure activated PPARγ nuclear hormone receptor interaction with SRCPs. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human ADRA2A β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human ADRA2A β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure ADRA2A (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter™ Human MIP-3 beta (CCR7) Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human MIP-3 beta (CCR7) Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CCR7 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress TYK2 Activity Assay, 10-Plate Kit. (93-0875E3CP16L)
The PathHunter eXpress TYK2 Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting the TYK kinase domain. The cell line is engineered to express the catalytic domain from TYK2 fused to the cytokine receptor binding domain (CRBD) of JAK2. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the GHR-PK. Activation of the GHR-PK induces receptor phosphorylation by the TYK2 catalytic domain. This leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Enzo Life Sciences CV-Cathepsin L detection kit (1 Kit)
Elevated cathepsin enzyme activity often indicates the presence of a pathological condition including cancer, Alzheimer's disease, autoimmune disease, and osteoporosis. Based on the dye cresyl violet and the cathepsin L PheArg peptide recognition sequence, this kit allows the detection of cathepsin L activity in living cells by fluorescence microscopy. Once cells are grown, staining is completed in just a few minutes. Hoechst 33342 stain and Acridine Orange are also included to allow visualization of nuclei and lysosomes. Applications: Fluorescence microscopy, Fluorescent detection .
Cayman Chemical Acid Phosphatase Colorimetric Assay Kit, 1 ea
Cayman’s Acid Phosphatase Assay provides a convenient method for detecting AP activity in plasma, serum, urine, and semen. The assay utilizes para-nitrophenyl phosphate (pNPP) as a chromogenic substrate for the enzyme. In the first step, AP dephosphorylates pNPP. In the second step, the phenolic OH-group is deprotonated under alkaline conditions resulting in p-nitrophenolate that yields an intense yellow color which can be measured at 405-414 nm. The kit provides all reagents needed to assay AP activity, and includes L-tartrate, an inhibitor of non-tartrate resistant APs.
Eurofins Discoverx PathHunter Human BMP-6 (BMPR1B/ACVR2B) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human BMP-6 (BMPR1B/ACVR2B) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of BMPR1B/ACVR2B. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human PTGER3 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human PTGER3 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure PTGER3 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery U-PLEX Human TNF-B ASY 25 PL
The Meso Scale Discovery U-PLEX Human TNF-beta Assay Kit is a multiplex immunoassay that measures human TNF-beta in serum plasma cell culture supernatants and other biological samples It utilizes electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and requires low sample volume The assay can be run on Meso Scale Discovery instrumentation including the QuickPlex SQ 120 QuickPlex SQ 120i MESO QuickPlex SQ 120 and V-PLEX Plus The kit includes a 96-well plate coated with capture antibodies detection antibodies calibrators and ancillary reagents needed to perform the assay
Eurofins Discoverx PathHunter eXpress TrkC-P75 Functional Assay, 10-Plate Kit. (93-0531E3CP16L)
The PathHunter eXpress TrkC-P75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkC and P75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkC and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, P75. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR109B CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0791E2CP2L)
The PathHunter GPR109B eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. 3-hydroxyoctanoic acid) based activation of GPR109B GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR109B and EA-tagged β-Arrestin-2. Activation of GPR109B stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mADCYAP1R1 (PAC1) CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0594E2MCP0L)
The PathHunter mADCYAP1R1 (PAC1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PACAP) based activation of mADCYAP1R1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mADCYAP1R1 and EA-tagged β-Arrestin-2. Activation of mADCYAP1R1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.