General Cell Based Assays
Lonza Walkersville PPiLight Inorganic Pyrophosphate Assay, 500 Test
Inorganic pyrophosphate (also referred to as diphosphate, pyrophosphoric acid or PPi) is a small diphosphate molecule that is required as a substrate or is the product formed from a number of different enzymatic reactions. Enzymes that utilize PPi as a substrate may include phosphotransferases (EC 2.7) and pyrophosphatases (EC 3.6.1.1). Enzymes that generate PPi are more numerous and may include cyclases (EC 4.6.1), hydrolyses (EC 3), ligases (EC 6) and DNA polymerases such as DNA Polymerase theta (θ) encoded by POLQ. Benefits:Fast - Measure enzyme activity via pyrophosphate consumption in 1 hourSimple - The 2 step, luminescent assay doesn’t require radioactive substrates, beads, detection antibodies or modified substratesWide Detection Range - Sensitive to 0.02 µM with a linear range to 10 µMVersatile - Scalable to 96-, 384-, 1586-well formatsApplications:Measure activity of:PhosphotransferasesPyrophosphotasesCyclasesLigasesHydrolasesDNA Polymerases
Sigma Aldrich Fine Chemicals Biosciences Hemoglobin Assay Kit sufficient for 250 colorimetric tests | 1KT
Hemoglobin Assay Kit sufficient for 250 colorimetric tests | 1KT
Eurofins Discoverx AssayComplete Cell Plating 2 Reagent, 500. (93-0563R2B)
AssayComplete Cell Plating 2 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
THEWELL BIOSCIENCE High Concentration Kit Type: VitroGel 3D + Dilution Solution TYPE 1
Dilution Solution TYPE 1 contains sucrose for best osmolarity.
Dojindo Molecular Technologies Inc DAPRed – Autophagy Detection (5 nmol)
DAPRed is used to detect autophagy in live cells. Autophagy is an intracellular degradation system, where dysfunctional proteins and organelles are degraded. In this process, aggregated dysfunctional proteins are surrounded by the double membrane to form an autophagosome. The small fluorescent molecule DAPRed is used to detect autophagosomes and autolysosomes. The mechanism suggests that the dye is incorporated into the autophagosome during double-membrane formation via structural features and then emits fluorescence under hydrophobic conditions. DALRed is cell-permeable, has no requirement of transfection method, and enables live-cell imaging with fluorescence microscopy. Excitation and emission of DAPRed: λex: 530 nm, λem: 720 nm. For monitoring only autolysosomes, we recommend DALGreen [D675] as it enables the detection of phagosome-lysosome fusion. Dojindo offers various reagents for autophagy research. (https://www.dojindo.com/autophagy-research/#jmp-b)
Epigentek Inc Epigenase HDAC Activity/Inhibition Direct Assay Kit (Colorimetric) (48 assays)
The Epigenase HDAC Activity/Inhibition Direct Assay Kit is a complete set of optimized buffers and reagents for measuring the activity or inhibition of total HDAC enzymes in nuclear extracts or purified HDAC isoforms (HDACs 1-11) from a broad range of species such as mammals, plants, fungi, and bacteria, and in a variety of forms including, but not limited to, cultured cells and fresh and frozen tissues. The Epigenase HDAC Activity/Inhibition Direct Assay Kit is a further refinement of its predecessor, the EpiQuik HDAC Activity/Inhibition Assay Kit, a popular assay method for HDAC activity and inhibition. This latest method retains the simplicity, rapidness, high throughput capability, and non-radioactivity featured in the previous version.