General Cell Based Assays
Eurofins Discoverx PathHunter Insulin Lispro Bioassay Kit, 2pl. (93-0466Y3-00009)
The PathHunter Insulin Lispro Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses insulin or insulin lispro based activation of INSRb RTK receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged INSRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx cAMP Hunter LHCGR Bioassay Kit, 2-Plate. (95-0106Y2-00137)
The cAMP Hunter CHO-K1 LHCGR Bioassay Kit provides a robust, and highly sensitive functional, cell-based assay to measure drug potency and detect neutralizing antibodies.. The bioassay kit contains all the materials needed for a complete assay, including cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The ready-to-use cryopreserved cells have been manufactured to ensure assay reproducibility and faster implementation from characterization to lot release. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter Human IL-15 (IL-15R (IL2RB/IL2RG)) Signaling Reporter Cell-Based Assay Kit (REH)
The PathHunter Human IL-15 (IL-15R (IL2RB/IL2RG)) Signaling STAT5 Reporter Stable Cell Line Assay (REH) monitor the activation of specific signaling pathways, utilizing the established PathHunter Enzyme Fragment Complementation (EFC) technology. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter™ Human DRD2L Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human DRD2L Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure DRD2L (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Immunochemistry Technology FLUORO NAD/NADH DETECTION KIT
The role of pyridine nucleotides (NAD/NADH) metabolism in health, is of continual and increased interest. A growing amount of evidence supports the fact that NAD metabolism regulates important biological effect including life span. NAD, through poly-ADP-ribosyl polymerase (PARP), mono-ADP-ribosyltransferase (ARTs) and recently characterized sirtuin enzymes, exerts potential biological effects. These enzymes modify proteins to regulate their function via ADP-ribosylation or deacetylation and are involved in several pathways including apoptosis, DNA repair, senescence and endocrine signaling. This suggests that either the enzymes or NAD could be an important therapeutical target 1.
Eurofins Discoverx PathHunter Human HTR5A (5-HT5A) β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human HTR5A (5-HT5A) β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure HTR5A (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress TBXA2R U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0322E3CP5M)
The PathHunter TBXA2R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. thromboxane A2, prostaglandins) based activation of TBXA2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged TBXA2R and EA-tagged β-Arrestin-2. Activation of TBXA2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HRH3 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0509E2CP2L)
The PathHunter HRH3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. histamine) based activation of HRH3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HRH3 and EA-tagged β-Arrestin-2. Activation of HRH3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human Oncostatin M (OSMR/IL6ST) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human Oncostatin M (OSMR/IL6ST) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of OSMR/IL6ST. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human F2R β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human F2R β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure F2R (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human HTR2C (5-HT2C) Activated GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human HTR2C (5-HT2C) Stable Cell Line Activated GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure HTR2C (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Echelon Biosciences Research Labs High Throughput Autotaxin Inhibitor Screening Kit
The Autotaxin Inhibitor Screening Kit utilizes purified autotaxin (ATX) and the autotaxin substrate FS-3, which fluoresces upon cleavage. The assay is performed in a 96 well plate and quantifies the autotaxin activity in the presence of the users samples by measuring changes in fluorescence over time.Sample Type: Inhibitors against ATXSample Volume: 10 L/ well of your sample compoundsSample Considerations: Some organic solvents interfere with the assay (see technical data sheet). This item is non returnable
Eurofins Discoverx AssayComplete Cell Plating 24 Reagent, 500. (93-0563R24B)
AssayComplete Cell Plating 24 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter Human PTGER4 Activated GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human PTGER4 Stable Cell Line Activated GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure PTGER4 (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human NPBWR2 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human NPBWR2 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure NPBWR2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.