General Cell Based Assays
Sigma Aldrich Fine Chemicals Biosciences Cytotoxicity Detection KitPLUS (LDH), 400 tests
Nonradioactive colorimetric assay suitable for high-throughput quantification of cell death and cell lysis, based on the measurement of lactate dehydrogenase (LDH) activity released from the cytosol of damaged cells into the supernatant. The Cytotoxicity Detection KitPLUS (LDH) can be performed in a homogeneous format and requires no transfer and centrifugation steps to separate the supernatant from the cells. The color reaction can be stopped for defined assay conditions.
Cell Biolabs Inc OxiSelect™ Total Antioxidant Capacity (TAC) Assay Kit, 200 assays
Cell Biolabs’ OxiSelect™ Total Antioxidant Capacity (TAC) Assay Kit measures the total antioxidant capacity of biomolecules from a variety of samples via a SET mechanism. The TAC Assay is based on the reduction of copper (II) to copper (I) by antioxidants such as uric acid. Upon reduction, the copper (I) ion further reacts with a coupling chromogenic reagent that produces a color with a maximum absorbance at 490 nm. Copper is advantageous over iron-based antioxidant assays because all classes of antioxidants, including thiols, are detected with marginal radical interference. Cell Biolabs’ OxiSelect™ TAC Assay Kit is a fast and reliable kit for the direct measurement of total antioxidant capacity from cell lysate, plasma, serum, urine, tissue homogenates, and food extracts. Each kit provides sufficient reagents to perform up to 200 assays, including blanks, antioxidant standards and unknown samples. Both hydrophilic and lipophilic samples are compatible with the assay.
