General Cell Based Assays
Molecular Devices FLIPR Calcium 6 Assay Kit, 10-Plate Explorer
The Molecular Devices FLIPR Calcium 6 Explorer Kit provides reagents for fluorescent intracellular calcium flux assays optimized for FLIPR-compatible high-throughput readers. The kit supplies enough reagent for ten assay plates and is engineered to deliver a high signal-to-background window for low-signal targets such as endogenous, primary, or stem cell assays.
- Explorer kit format supplies reagents for 10 assay plates.
- Compatible with 96-well, 384-well, and 1536-well plate formats.
- Includes 10 vials of Component A and 1 bottle of dilution/assay buffer (Component B).
- Optimized calcium indicator with high quantum yield for strong signal-to-background.
- Suitable for FLIPR-compatible plate readers and high-throughput calcium flux screening.
Eurofins Discoverx PathHunter Human AVPR2 (S167T) ENDO-EA Pharmacotrafficking Cell-Based Assay Kit (U2OS)
The PathHunter Human AVPR2 (S167T) ENDO-EA Pharmacotrafficking Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure pharmacochaperone-based rescue of the mutant AVPR2's trafficking to early endosomes. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery U-PLEX Mouse IL-5 ASY
The Meso Scale Discovery U-PLEX Mouse IL-5 Assay Kit is an electrochemiluminescent immunoassay for quantifying mouse interleukin 5 (IL-5) in serum plasma and cell culture supernatants It uses MSD s SECTOR Imager detecting IL-5 from 0 04 to 1000 pg/mL The kit includes a 96-well plate with capture antibody detection antibody with MSD SULFO-TAG calibrators and diluent The assay takes 3 5 hours and supports research on mouse models of asthma allergies and other IL-5-related immunological diseases
Eurofins Discoverx PathHunter eXpress GPR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0335E2CP2L)
The PathHunter GPR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. chemerin) based activation of GPR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR1 and EA-tagged β-Arrestin-2. Activation of GPR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Total Antioxidant Capacity Assay Kit.
Total Antioxidant Capacity Assay Kit.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Protease Activity Assay Kit
Protease Activity Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress mEDNRA CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0685E2MCP0L)
The PathHunter mEDNRA eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. endothelin-1) based activation of mEDNRA GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mEDNRA and EA-tagged β-Arrestin-2. Activation of mEDNRA stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Bioassay Detection Kit, 500 (10,000 dp, 96-well). (93-0933L)
The PathHunter Bioassay Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter Bioassay cells or cell lines. This kit contains lysis buffer and EFC substrate to allow for detection of EFC levels in the assayed cells. This kit contains all components required to assay 100 plates (96-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-0933l.
Indigo Biosciences INDIGO Biosciences Activation Reporter Assay System, Human CHRM1, 96-well
This Human Cholinergic Receptor, Muscarinic 1 (CHRM1) Activation Reporter Assay System is a cell-based assay for screening compounds. It quantifies changes in luciferase activity to measure drug-induced changes in CHRM1 activity, identifying activators of CHRM1 or the coupled Ca+2-calcineurin / NFAT signal transduction pathway.
- Utilizes proprietary human cells engineered to constitutively express the human cholinergic receptor, muscarinic 1 (CHRM1).
- Measures drug-induced changes in CHRM1 activity through luciferase reporter gene expression.
- Designed for screening compounds that activate CHRM1 or the Ca+2-calcineurin / NFAT signal transduction pathway.
- Provided in a convenient 96-well format for high-throughput screening.
- Kit includes reporter cells, media, acetylcholine, detection reagents, and a collagen-coated assay plate.
Cayman Chemical 8-cPeT NAD sodIum salt 800ug
A derivative of NAD+; has been used in the screening of analog-sensitive PARP1 gatekeeper mutations
Eurofins Discoverx PathHunter eXpress GALR1 Total Internalization Assay, 10pl Kit. (93-0781E3CP5L)
The PathHunter GALR1 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GALR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged GALR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the GALR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ADRA2B CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0641E2CP2L)
The PathHunter ADRA2B eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2B GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged ADRA2B and EA-tagged β-Arrestin-2. Activation of ADRA2B stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress NPBWR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0818E2CP2L)
The PathHunter NPBWR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neuropeptides B/W) based activation of NPBWR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NPBWR1 and EA-tagged β-Arrestin-2. Activation of NPBWR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Enzo Life Sciences HDAC substrate (fluorogenic) (1mg). CAS: 233691-67-3
A fluorogenic substrate for histone deacetylase (HDAC). Ex(max): 390nm; Em(max): 460nm. Alternative name: Boc-Lys(Ac)-AMC, MAL, Histone deacetylase substrate (fluorogenic), N-(4-Methyl-7-aminocoumarinyl)-Nalpha-(t-butoxycarbonyl)-Nomega-acetyllysineamide. Formula: C23H31N3O6. MW: 445.5. Purity: ≥99%. Appearance: White crystalline solid. Solubility: Soluble in DMSO and 100% ethanol (1mg/ml). Long Term Storage: -20°C. Handling: Protect from light.
Eurofins Discoverx PathHunter PDGFRa Bioassay Kit, 2-Plate. (93-0823Y3-00071)
The PathHunter PDGFRa Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This cell line enables assessment of ligand (e.g. PDGF-BB) based activation of PDGFRa receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged PDGFRa target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.