Protein Purification
Promega NanoBRET™ Nano-Glo™ Detection System
The NanoBRET Nano-Glo™ Detection System provides the NanoBRET Nano-Glo™ Substrate used by NanoLuc Luciferase to generate the donor signal and the HaloTag™ NanoBRET 618 Ligand for the fluorescent energy acceptor.
Promega HaloTag™ PEG-Biotin Ligand
The HaloTag™ Technology enables covalent protein labeling and immobilization in vivo and in vitro.
Promega NanoBRET™ Nano-Glo™ Substrate
The NanoBRET™ Nano-Glo(R) Substrate is the substrate used by NanoLuc(R) Luciferase to generate the donor signal in several NanoBRET™ Assays.
Promega Proteasome-GLO™ Cell-Based Assay System
The Proteasome-Glo™ Cell-Based Assays are homogeneous, luminescent assays that measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in cultured cells.
Promega NanoBRET™ Positive Control
The NanoBRET Positive Control Vector encodes a NanoLuc-HaloTag™ fusion protein that tethers together the NanoLuc donor and HaloTag™ acceptor proteins to ensure efficient energy transfer.
Promega Proteasome-Glo™ Assays
The Proteasome-Glo™ Cell-Based Assays are homogeneous, luminescent assays that measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in a purified enzyme preparation.
Promega NanoBRET™ PPI Control Pair (p53, MDM2)
The NanoBRET PPI Control Pair (p53, MDM2) is an optimized pair of vectors designed to measure the interaction of protein partners p53 and MDM2 using the NanoBRET assay.
Promega HaloTag™ Mammalian Protein Purification System
HaloTag™ Mammalian Protein Purification System is an optimized kit for purification of HaloTag™ fusion proteins from mammalian cell culture lysates. HaloTag™ Mammalian Protein Detection and Purification System also includes HaloTag™ TMRDirect Ligand.
Promega FastBreak™ Cell Lysis Reagent
FastBreak Cell Lysis Reagent allows efficient, gentle lysis of E. coli cultures without the need for centrifugation or mechanical disruption.
Promega NanoBRET™ cMyc/MAX Interaction Assay
NanoBRET Transcriptional Protein Assays are sensitive, reproducible live-cell assays designed for monitoring or screening the interaction of proteins involved in transcriptional regulation.
Promega pFC30K His6HaloTag™ T7 Flexi Vector for E. coli and Cell-Free Protein Expression
These vectors are used for inducible expression of polyhistidine-HaloTag™ fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.
Promega Goat Anti-Human Biotinylated IgG, 4ml
High Capacity Magne Streptavidin Beads are magnetic affinity beads with high specificity and high capacity for binding biotinylated antibodies and proteins. Goat Anti-Human Biotinylated IgG is 0.5mg/ml in PBS (pH 7.4) with 0.1% sodium azide.
Promega pFN29A His6HaloTag™ T7 Flexi T7 Vector for E. coli and Cell-Free Protein Expression
These vectors are used for inducible expression of polyhistidine-HaloTag™ fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.
Promega HaloTag™ NanoBRET™ 618 Ligand
NanoBRET Transcriptional Protein Assays are sensitive, reproducible live-cell assays designed for monitoring or screening the interaction of proteins involved in transcriptional regulation.