Protein Purification
Promega NanoBRET™ PPI MCS Starter System
Protein:Protein Interaction (PPI) Assays use NanoLuc Luciferase and HaloTag™ protein fused to target proteins of interest to enable sensitive, reproducible detection of protein interactions in the natural cellular environment.
Promega HaloTag™ Biotin Ligand
The HaloTag™ Technology enables covalent protein labeling and immobilization in vivo and in vitro.
Promega NanoBRET™ Nano-Glo™ Substrate
The NanoBRET™ Nano-Glo(R) Substrate is the substrate used by NanoLuc(R) Luciferase to generate the donor signal in several NanoBRET™ Assays.
Promega Magne™ HaloTag™ Magnetic Affinity Beads
The Magne HaloTag™ Beads provide a convenient method to covalently capture HaloTag™ fusion proteins with magnetic particles for protein pull-downs and purification. The beads offer high binding capacity with low nonspecific protein binding.
Promega HaloTag™ PEG-Biotin Ligand
The HaloTag™ Technology enables covalent protein labeling and immobilization in vivo and in vitro.
Promega NanoBRET™ Nano-Glo™ Detection System
The NanoBRET Nano-Glo™ Detection System provides the NanoBRET Nano-Glo™ Substrate used by NanoLuc Luciferase to generate the donor signal and the HaloTag™ NanoBRET 618 Ligand for the fluorescent energy acceptor.
Promega pFN29K His6HaloTag™ T7 Flexi Vector for E. coli and Cell-Free Protein Expression
These vectors are used for inducible expression of polyhistidine-HaloTag™ fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.
Promega E. coli S30 T7 High-Yield Protein Expression System
The E. coli S30 T7 High-Yield Protein Expression System is designed to express up to 500ug/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site.
Promega Proteasome-GLO™ Cell-Based Assays
The Proteasome-Glo™ Cell-Based Assays are homogeneous, luminescent assays that measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in cultured cells.
Promega SoftLink™ Soft Release Avidin Resin
SoftLink Avidin Resin is used for the isolation and purification of biotinylated molecules.
Promega NanoBRET™ BRD4/Histone H3.3 Interaction Assay
The NanoBRET Bromodomain BRD4/Histone Interaction Assays enable interaction studies of BRD-containing proteins with full-length histones in the context of natural chromatin.
Promega High Capacity Magne Streptavidin Beads, 3ml
High Capacity Magne Streptavidin Beads are magnetic affinity beads with high specificity and high capacity for binding biotinylated antibodies and proteins. Goat Anti-Human Biotinylated IgG is 0.5mg/ml in PBS (pH 7.4) with 0.1% sodium azide.
Promega Protease Inhibitor Cocktail
Protease Inhibitor Cocktail is used to prevent protein degradation after lysing cells. The product is a mixture of six different protease inhibitors with different target protease specificities.
Promega HaloTEV™ Protease
HaloTEV Protease (81kDa) is a fusion between the HaloTag™ protein and TEV protease, a highly specific proteolytic enzyme that cleaves at a specific TEV site, a specific seven-amino-acid sequence (ENLYFQ(G/S)).
Promega MagneHis™ Protein Purification System
The MagneHis Protein Purification System provides a magnetic-based, rapid method for the purification of polyhistidine- or HQ-tagged, expressed proteins.
