Protein Purification
STEMCELL Technologies RoboSep™ Human PE Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human PE Positive Selection Kit II is designed to isolate cells that are labeled with PE (phycoerythrin)-conjugated antibodies by positive selection. Desired cells are targeted with antibody complexes recognizing PE and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Human PE Positive Selection Kit (Catalog #18551). For highly purified particle-free cells, we recommend the EasySep™ Release Human PE Positive Selection Kit (Catalog #17654) (https://www.stemcell.com/products/easysep-release-human-pe-positive-selection-kit.html)
STEMCELL Technologies RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II, For processing: 60 mL whole blood or bone marrow processed
EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips
STEMCELL Technologies RoboSep™ Human Naïve B Cell Enrichment Kit with Filter Tips, For processing: 1 x 10^9 cells
The EasySep™ Human Naïve B Cell Enrichment Kit is designed to isolate naïve B cells from peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-naïve B cells and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. The desired cells are poured off into a new tube.
STEMCELL Technologies RoboSep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit, For processing: 60 mL whole blood processed
The EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection is designed to isolate CD4+ cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD4 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD4 antigen is expressed on helper T cells and more weakly on monocytes and tissue macrophages.This product replaces the EasySep™ Human Whole Blood CD4 Positive Selection Kit (Catalog #18082) for even faster cell isolations.
STEMCELL Technologies EasySep™ HLA Chimerism BC CD56 Positive Selection Kit, For processing: 30 mL buffy coat processed
The EasySep™ HLA Chimerism Buffy Coat CD56 Positive Selection Kit II is designed to isolate CD56+ cells from freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD56 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD56 antigen is expressed on NK cells and NKT cells.This product replaces the EasySep™ Human Buffy Coat CD56 Positive Selection Kit (Catalog #18085) and EasySep™ HLA Buffy Coat CD56 Positive Selection Kit (Catalog #18085HLA), for even faster cell isolations.
STEMCELL Technologies EasySep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Kit, For processing: 60 mL buffy coat or whole blood processed
The EasySep™ Human Whole Blood Lymphoid Positive Selection Kit is designed to isolate CD3+ and CD19+ cells from whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD3, CD19 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD3 antigen is expressed on T lymphocytes and NKT cells while the CD19 antigen is expressed on B cells and follicular dendritic cells.This product replaces the EasySep™ Human Whole Blood Lymphoid Positive Selection Kit (Catalog #18684) and EasySep™ HLA Whole Blood Lymphoid Positive Selection Kit (Catalog #18684HLA) for even faster cell isolations.
STEMCELL Technologies EasySep™ Human TCR Alpha/Beta Depletion Kit, undefined: 1 Kit
The EasySep™ Human TCR Alpha/Beta Depletion Kit is designed to deplete human T cell receptor alpha/beta+ (TCRab+) cells from leukapheresis samples or expanded TCRa/b knockout cell cultures. TCRab+ cells are targeted with antibody complexes and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Labeled cells remain in the tube while unlabeled cells are poured off.
STEMCELL Technologies RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit, For processing: 60 mL buffy coat or whole blood processed
Isolate highly purified CD33+ and CD66b+ myeloid cells, including myeloid progenitor cells, granulocytes, and monocytes, from fresh whole blood prior to chimerism testing. Desired cells are targeted with tetrameric antibody complexes recognizing CD33, CD66b, and dextran-coated magnetic particles, and separated without the use of columns through immunomagnetic positive selection using an EasySep™ magnet. Cells of interest remain in the tube while unwanted cells are poured off. This product can be used with RoboSep™ to automate immunomagnetic cell isolation. Visit www.EasySep.com for more information.
STEMCELL Technologies RoboSep™ Mouse Naïve CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit is designed to isolate naïve CD4+ T cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-naïve CD4+ T cells (CD8, CD11b, CD11c, CD19, CD24, CD25, CD44, CD45R, CD49b, TCRγ/δ, TER119) and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.Learn more about our next-generation EasySep™ mouse cell isolation kits (/easysep-15-minute-kits), featuring RapidSphere™ technology.
STEMCELL Technologies RoboSep™ Mouse Neutrophil Enrichment Kit with Filter Tips, For processing: 1 x 10^9 cells
The EasySep™ Mouse Neutrophil Enrichment Kit is designed to isolate neutrophils from single-cell suspensions of bone marrow or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies that are directed against non-neutrophils. Labeled cells are then recognized by Tetrameric Antibody Complexes that are directed against biotin and dextran. These cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
STEMCELL Technologies RoboSep™ Mouse CD19 Positive Selection Kit II, For processing: 2 x 10^9 cells
The EasySep™ Mouse CD19 Positive Selection Kit II is designed to isolate CD19+ cells from single-cell suspensions of splenocytes or other tissues by positive selection. Desired cells are targeted with antibody complexes recognizing CD19 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Mouse CD19 Positive Selection Kit (Catalog #18754) for even faster cell isolations and does not result in the labeling of isolated cells with PE.
STEMCELL Technologies EasySep™ Mouse Memory CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse Memory CD4+ T Cell Isolation Kit is designed to isolate pan-memory CD4+ T cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-memory CD4+ T cells (CD8, CD11b, CD11c, CD19, CD24, CD45R, CD45RB, CD49b, CD105 and TCRγ/δ) and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.Learn more about our next-generation EasySep™ mouse cell isolation kits (/easysep-15-minute-kits), featuring RapidSphere™ technology.
STEMCELL Technologies RoboSep™ Mouse CD11c Positive Selection Kit II with Spleen Dissociation Medium, For processing: 2 x 10^9 cells
The EasySep™ Mouse CD11c Positive Selection Kit II isolates highly purified CD11c+ cells from splenocytes or other tissues by immunomagnetic positive selection. Desired cells are targeted with antibodies and magnetic particles, and isolated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately ready for downstream applications such as flow cytometry, culture, and cell-based experiments.This product replaces the EasySep™ Mouse CD11c Positive Selection Kit (Catalog #18758) for even faster cell isolations and does not result in the labeling of isolated cells with PE.
STEMCELL Technologies RoboSep™ Human Pan-B Cell Enrichment Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Pan-B Cell Enrichment Kit is designed to isolate B cells including plasma cells from normal fresh or previously frozen peripheral blood mononuclear cells by negative selection. Undesired cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD14, CD16, CD36, CD42b, CD56, CD66b, CD123, glycophorin A and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
STEMCELL Technologies RoboSep™ Human Naïve CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Naïve CD4+ T Cell Isolation Kit is designed to isolate naive CD4+ T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes and dextran-coated magnetic particles. CD45RO+ cells are targeted for removal with a biotinylated anti-CD45RO antibody, and a bispecific Tetrameric Antibody Complex that recognizes biotin and dextran. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.For even faster cell isolations, we recommend the new EasySep™ Human Naïve CD4+ T Cell Isolation Kit II (17555) (https://www.stemcell.com/products/easysep-human-naive-cd4-t-cell-isolation-kit-ii.html) which isolates cells in just 11 minutes.