Protein Purification Reagents and Kits
Neuromics, Inc Strep-TactinXT 4Flow Resin, 20 ml is used for purification of recombinant Strep-tagII and Twin-Strep-tag fusion proteins.
Strep-TactinXT 4Flow is used for purification of recombinant Strep-tagII and Twin-Strep-tag fusion proteins under gravity flow and low pressure or FPLC.Dynamic binding capacity: 7 mg protein/ml resin, determined with mCherry-Twin-Strep-tag (30kDa).
IBA LifeSciences Strep-Tactin; 5 mg
Strep-Tactin is a streptavidin variant, which can be utilized for coating of different surfaces. It is optimized for the binding of Strep-tagII and Twin-Strep-tag fusion proteins but still maintains the ability to capture biotinylated targets. Therefore, it can be used for assay development and protein interaction studies, including experiments such as proximity-dependent biotin identification (BioID).
Medchemexpress LLC MUSK Mouse Myc Hi 5ug
Recombinant mouse MUSK protein bearing N-terminal Myc and His-SUMO tags, supplied lyophilized for biochemical and biophysical studies. The product is a fragment corresponding to residues E22-S494, expressed in E. coli, and formulated for stability and downstream assays such as SPR after buffer exchange.
- Recombinant fragment covering residues E22-S494.
- Expressed in E. coli and supplied lyophilized for long-term storage.
- Endotoxin level below 1 EU/μg as measured by LAL.
- Lyophilized from Tris-EDTA with trehalose for enhanced stability.
- Recommended reconstitution at not less than 100 μg/mL; buffer exchange suggested for SPR.
- Approximate molecular weight 69.3 kDa.
Sigma Aldrich Fine Chemicals Biosciences C myc proto oncogene human
MYCnbsponcogene is a global transcription factor. The gene is located on human chromosome 8q24.21. MYCnbsponcogene is a cancer promoting gene.
IBA LifeSciences Strep-TactinXT 4Flow resin; 50 ml
Strep-TactinXT 4Flow is a 4% agarose coupled with the streptavidin variant Strep-TactinXT and applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose allows for the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow enables the application of denaturing agents such as urea. The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography, rather than for batch purification. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.
Medchemexpress LLC MUSK Mouse Myc Hi 50ug
Recombinant fragment of mouse Muscle-Specific Kinase (MuSK) with Myc and His-SUMO tags supplied as a lyophilized powder. The protein is expressed in E. coli and formulated for stability, with low endotoxin and manufacturer guidance for reconstitution, storage, and handling available in the product datasheet.
- Recombinant fragment covering amino acids E22-S494.
- Myc and His-SUMO affinity tags for detection and purification.
- Expressed in E. coli for high yield production.
- Low endotoxin level (<1 EU/μg) suitable for cell-based assays.
- Lyophilized formulation for extended shelf life and easier shipping.
- Stable at -20°C for up to two years from receipt when stored properly.
Miltenyi Biotec, Inc. CD8+ DC Isolation Kit, mouse
Isolation of CD8+CD11c+ mouse dendritic cells
STEMCELL Technologies EasySep™ Mouse CD90.2 Positive Selection Kit II, For processing: 2 x 10^9 cells
The EasySep™ Mouse CD90.2 Positive Selection Kit II is designed to isolate CD90.2+ (Thy1.2+) cells from single-cell suspensions of splenocytes or other tissues by positive selection. Desired cells are targeted with antibody complexes recognizing CD90.2 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Mouse CD90.2 Positive Selection Kit (Catalog #18751) for even faster cell isolations and does not result in the labeling of isolated cells with PE.
STEMCELL Technologies RoboSep™ Human Neutrophil Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Neutrophil Isolation Kit is designed to isolate neutrophils from a polymorphonuclear cell-rich fraction of peripheral blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-neutrophils and magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. The isolation kit is compatible with cells prepared using HetaSep™ (Catalog #07906) sedimentation or red blood cell lysis.This product replaces the EasySep™ Human Neutrophil Enrichment Kit (Catalog #19257), for even faster cell isolations.
STEMCELL Technologies EasySep™ Direct Human Monocyte Isolation Kit, For processing: 100 mL whole blood processed
The EasySep™ Direct Human Monocyte Isolation Kit isolates functional, highly purified CD14+ monocytes directly from human whole blood by immunomagnetic negative selection. No lysis, density gradient centrifugation or other processing steps are required and isolated cells are immediately available for flow cytometry, functional assays, culture and other downstream applications.
TRIBIOSCIENCE INC Mitochondrial Isolation from Tissue and Cell, 50 Tests
Tribioscience’s Mitochondrial Isolation Kit provides an easy, simple approach for the isolation of intact and high quality mitochondria from tissues and cultured cells.