Protein Purification Reagents and Kits
American Research Products Inc Rat Granulocyte Macrophage Col
Rat Granulocyte-Macrophage Colony Stimulating Factor GM-CSF ELISA Kit from CUSABIO Detection Range 15 6 pg/ml - 1000 pg/ml Sensitivity 3 9 pg/ml Sample serum plasma tissue homogenates Method Sandwich
STEMCELL Technologies RosetteSep™ Human Cord Blood Debulking Cocktail, For processing: 200 mL cord blood processed
The RosetteSep™ Human Cord Blood Debulking Cocktail is designed to deplete lineage positive cells from fresh cord blood. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD14, CD19, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The hematopoietic stem and progenitor cells are present as a pre-enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies RoboSep™ Human Resting CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Resting CD4+ T Cell Isolation Kit is designed to isolate resting CD4+ T cells from fresh or previously frozen human peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
STEMCELL Technologies RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II, For processing: 60 mL whole blood or bone marrow processed
EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips
Sino Biological Human CD4 / LEU3 Protein (aa 1-208, His Tag), Endotoxin-Free 100µg
A DNA sequence encoding the human CD4 (NP_000607.1) N-terminal two domains (D1D2) (Met 1-Ser 208) was fused with a polyhistidine tag at the C-terminus.
STEMCELL Technologies RoboSep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Kit, For processing: 60 mL buffy coat or whole blood processed
The EasySep™ Human Whole Blood Lymphoid Positive Selection Kit is designed to isolate CD3+ and CD19+ cells from whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD3, CD19 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD3 antigen is expressed on T lymphocytes and NKT cells while the CD19 antigen is expressed on B cells and follicular dendritic cells.This product replaces the EasySep™ Human Whole Blood Lymphoid Positive Selection Kit (Catalog #18684) and EasySep™ HLA Whole Blood Lymphoid Positive Selection Kit (Catalog #18684HLA) for even faster cell isolations.
STEMCELL Technologies RoboSep™ Human Monocyte Enrichment Kit with Filter Tips, For processing: 1 x 10^9 cells
The EasySep™ Human Monocyte Enrichment Kit is designed to isolate monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-monocyte cells and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to prevent nonspecific binding of monocytes. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For applications in which CD16+ cells are not removed, we recommend the EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion (Catalog #19058). For even faster cell isolations, we recommend the new EasySep™ Human Monocyte Isolation Kit (19359) (http://www.stemcell.com/en/Products/All-Products/EasySep-Human-Monocyte-Isolation-Kit.aspx) which isolates cells in as little as 12.5 minutes.
STEMCELL Technologies RoboSep™ Mouse Naïve CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit is designed to isolate naïve CD4+ T cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-naïve CD4+ T cells (CD8, CD11b, CD11c, CD19, CD24, CD25, CD44, CD45R, CD49b, TCRγ/δ, TER119) and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.Learn more about our next-generation EasySep™ mouse cell isolation kits (/easysep-15-minute-kits), featuring RapidSphere™ technology.
STEMCELL Technologies EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
This kit isolates human CD62L+CD45RO+ and CD62L-CD45RO+ memory CD4+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples. First, CD62L+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD62L+ cells, and unwanted CD45RA+ and non-CD4+ T cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified CD62L+CD45RO+ central memory CD4+ T cells that are immediately ready for downstream applications. Effector memory CD4+ T cells can be isolated from the CD62L- fraction.
STEMCELL Technologies EasySep™ Mouse Plasmacytoid DC Isolation Kit, For processing: 2 x 10^9 cells
The EasySep™ Mouse Plasmacytoid DC Isolation Kit is designed to isolate plasmacytoid dendritic cells (pDCs) from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies that are directed against non-pDCs. Labeled cells are then recognized by Tetrameric Antibody Complexes that are directed against biotin and dextran. These cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
STEMCELL Technologies Mitochondrial Isolation Kit, Size: 50 Tests
Conveniently enrich the mitochondrial or cytosolic subcellular fractions from mammalian cells or tissues using the Mitochondrial Isolation Kit. This kit offers two quick options to isolate the mitochondrial subcellular fraction and has been verified for use with western blot analysis. The first option is a reagent-based method for processing up to six samples concurrently. The second option utilizes Dounce homogenization, providing a two-fold increase in isolated mitochondria from a single sample. Both options use a microcentrifuge-based method to separate the mitochondrial fraction from the cytosolic fraction while preserving their biological activity. This separation is a valuable tool for studying apoptosis and signaling events between the two fractions. For more information on the Mitochondrial Isolation Kit, visit www.stemcell.com.
STEMCELL Technologies EasySep™ Direct Human Eosinophil Isolation Kit, For processing: 100 mL whole blood processed
The EasySep™ Direct Human Eosinophil Isolation Kit isolates functional, highly purified eosinophils directly from human whole blood by immunomagnetic negative selection. No lysis, density gradient centrifugation, or other processing steps are required and isolated cells are immediately available for flow cytometry, functional assays, culture, and other downstream applications.
STEMCELL Technologies EasySep™ Release Human PE Positive Selection Kit, Size: 1 x 10^9 cells
Immunomagnetic positive selection of particle-free human cells labeled with PE-conjugated antibodies
STEMCELL Technologies EasySep™ FITC Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ FITC Positive Selection Kit II is designed to isolate cells that are labeled with FITC-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing FITC and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ FITC Positive Selection Kit (Catalog #18558) for even faster cell isolations.
STEMCELL Technologies EasySep™ Mouse CD19 Positive Selection Kit II, For processing: 2 x 10^9 cells
The EasySep™ Mouse CD19 Positive Selection Kit II is designed to isolate CD19+ cells from single-cell suspensions of splenocytes or other tissues by positive selection. Desired cells are targeted with antibody complexes recognizing CD19 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Mouse CD19 Positive Selection Kit (Catalog #18754) for even faster cell isolations and does not result in the labeling of isolated cells with PE.