Protein Purification Reagents and Kits
Bioworld BLUE SEPAROPOREAGAROSE) 6
Blue Separopore (Agarose) 6B-CL. Technical Specifications: Ligand: Cibacron Blue F3G-A; Matrix: Separopore 6B-CL (crosslinked agarose, 6%); Particle size range: 52 - 165 um; Matrix activation: Triazine coupling; Coupling conditions: pH 9 - 10; Ligand density: 8 umol; Cibacron Blue/ml drained gel; Binding capacity: > 15 - 20 mg bovine serum albumin/ml drained gel; pH stability: 3 - 13; Chemical stability: Stable to all commonly used aqueous buffers and additives such as 8M urea and 6M guanidine hydrochloride.Physical stability: Negligible volume variation due to changes in pH or ionic strength.Supplied as Suspension in 0.5M NaCl containing 0.02% thimerosal; Storage: 2 - 8 deg.C. 25ml.
STEMCELL Technologies EasySep Human Bone Marrow CD13, 1 Kit
Immunomagnetic positive selection of human CD138 cells from bone marrow samples for in vitro diagnostic IVD applications
Macherey-Nagel NucleoSpin TriPrep, 50 preps
NucleoSpin TriPrep (50) 50 preps for the parallel purification of RNA, DNA, and protein from one sample - NucleoSpin TriPrep Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase
AdipoGen NUCB2 mouse rec His
Protein. Mouse nucleobindin-2 aa 25-420 is fused at the C-terminus to a His-tag. Source E. coli. Endotoxin content <1EU/µg purified protein LAL test Lonza. Liquid. 0.2µm-filtered solution in PBS, pH 7.2. Purity >90% SDS-PAGE. Nucleobindin-2 NUCB2 Prepronesfatin was first isolated in adipocytes, but is also expressed by gastric mucosa and pancreatic beta cells. This pattern of nucleobindin-2 expression, as well as the presence of nucleobindin-2 within the plasma of rodents and humans, strongly suggests that this compound may act as a circulating regulatory factor. NUCB2 can be cleaved into three polypeptides nesfatin-1, nesfatin-2, and nesfatin-3. Anorexigenic effects have been described for nesfatin-1 that plays an important role in hypothalamic pathways regulating food intake and energy homeostasis and acting in a leptin-independent manner. Nesfatin-1 may also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance.
AdipoGen DNER ED h rec
Protein. Signal peptide and extracellular domain of human DNER aa 1-640 are fused at the C-terminus with a FLAG -tag. Source HEK 293 cells. Endotoxin content <0.1EU/µg purified protein LAL test Lonza. Lyophilized. Contains PBS 0.02% CHAPS. Purity >90% SDS-PAGE. DNER is an activator of the Notch1 pathway. It mediates neuron-glia interaction during astrocytogenesis.
STEMCELL Technologies RoboSep™ CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit, For processing: 1 x 10^9 cells
The EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit is designed to isolate CD4+CD127lowCD49d- cells from fresh peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-CD4+ T cells, CD127high, CD49d+ cells and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. The desired cells are poured off into a new tube.
STEMCELL Technologies EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit, For processing: 1 x 10^9 cells
This kit isolates human CD4+CD127lowCD25+ regulatory T cells (Tregs) from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or leukapheresis samples. First, CD25+ cells are isolated by column-free immunomagnetic positive selection using EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD25+ cells, and unwanted non-Tregs are targeted for depletion. The final isolated fraction contains highly purified CD4+CD127lowCD25+ cells that express high levels of FOXP3 and are immediately ready for downstream applications. An optional protocol allows for the isolation of CD4+CD25- responder T cells in parallel for use in functional studies. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.
STEMCELL Technologies RoboSep™ Human CD33 Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human CD33 Positive Selection Kit II is designed to isolate CD33+ cells from ammonium chloride lysed blood or bone marrow, or from nucleated cells prepared by density separation with RosetteSep™ DM-M density medium (Catalog #15725) by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD33 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD33 antigen is expressed on cells of the myeloid lineage.This product replaces the EasySep™ Human CD33 Positive Selection Kit (Catalog #18257) for even faster cell isolations.
Takara Bio AAVPRO PURIFICATION KIT MIDI
AAVpro Purification Kit Midi (All Serotypes),4 Preps.The AAVpro Purification Kit Midi (All Serotypes) is a four-prep kit that can be used for purifying AAV of any serotype without the need for ultracentrifugation. The purification yields highly pure AAV particles that can be used directly for in vivo transduction or transduction of cultured cells. Cryonase Cold-Active Nuclease is included. This product allows for purification of four virus preps, each from a culture size of 1 x T225 flask.
AdipoGen Zmp1 M tuberculosis His
Protein. Full-length Zmp1 Mycobacterium tuberculosis is fused at the N-terminus to a His-tag. Source E. coli. Endotoxin content N/A. Liquid. In 10mM TRIS-HCl, pH 8.0. Prefered cleavage sequence is PxxF. Purity >98% SDS-PAGE. M13 endopeptidases are single-pass, transmembrane zinc-metallopeptidases responsible in human for the regulation of the biological activity of many hormones and peptides and are involved in many important processes such as blood pressure regulation Neprilysin or NEP1, cardiovascular development Endothelin Converting Enzyme-1 or ECE-12, prevention of haemolytic reaction KELL and phosphate homeostasis PHEX. Zmp1 is a soluble enzyme member of the M13 endopeptidases family that plays a key role in Mycobacterium tuberculosis pathogenicity with a still unknown mechanism.
Revvity Health Sciences Inc PhenoVue DRAQ7Â Dead Cell Nuclear Stain, 1 mLÂ
PhenoVue DRAQ7Â Dead Cell Nuclear Stain, 1 mLÂ
Abcam ChIP Kit Magnetic - One Step, 48 Tests
Abcam's ChIP Kit Magnetic - One Step (ab156907) is a complete set of optimized buffers and reagents to carry out a successful chromatin immunoprecipitation for the selective enrichment of a chromatin fraction containing specific DNA sequences from isolated chromatin, in order to investigate the interaction between proteins and DNA in a cell.
The product is subject to the following: Abcam Restricted Use Statement
Bioworld Co-IMAC 5 x 1mL Columns, 5 Column
Co-immobilized metal affinity chromatography (IMAC) columns consist of Co-Chelated Separoporematrix conveniently prepacked spin columns for gravity flow chromatography.Co-IMAC Columns separate proteins via their differences in affinity toCo2+ metal ions. Ideal for purification of recombinant proteins containing (His)6 fusion tag.Technical SpecificationsImminodiacetate immobilized matrix charged withCo metal ions.Chelating group coupling method: epoxy activationMatrix: cross-linked Separopore 4BParticle size (range): 50-170µm Metal ion capacity: > 20µmol Cu2+/ml gelSpacer arm: 14 atompH stability: 3-12Binding Capacity: Approx. 40 mg His-tagged protein/ml mediumSupplied as suspension in 20% ethanol.
Bioworld Zn-IMAC 5 x 2mL Columns, 5 Column
Zn-immobilized metal affinity chromatography (IMAC) columns consist of Zn-Chelated Separoporematrix conveniently prepacked spin columns for gravity flow chromatography.Zn-IMAC Columns separate proteins via their differences in affinity toZn2+ metal ions. Ideal for purification of recombinant proteins containing (His)6 fusion tag.Technical SpecificationsImminodiacetate immobilized matrix charged withZn metal ions.Chelating group coupling method: epoxy activationMatrix: cross-linked Separopore 4BParticle size (range): 50-170µm Metal ion capacity: > 20µmol Zn2+/ml gelSpacer arm: 14 atompH stability: 3-12Binding Capacity: Approx. 40 mg His-tagged protein/ml mediumSupplied as suspension in 20% ethanol.