Protein Purification Reagents and Kits
Bioworld DNA Denaturing Solution, 1 L
Denaturing Solution for use in Acrylamide Gel ElectrophoresisSpecificationContains: 0.5N NaOH and 1.5M NaCl
STEMCELL Technologies RoboSep™ Human Eosinophil Isolation Kit, For isolating: 1 x 10^9 cells
The EasySep™ Human Eosinophil Isolation Kit is designed to isolate eosinophils from a polymorphonuclear cell-rich fraction of peripheral blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-eosinophils and magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. The kit is compatible with cells prepared using either Lymphoprep™ (Catalog #07801) centrifugation (preferred for higher cell purities) or HetaSep™ (Catalog #07906) sedimentation (preferred for faster, lysis-free sample processing).This product replaces the EasySep™ Human Eosinophil Enrichment Kit (Catalog #19256) for even faster cell isolations.
STEMCELL Technologies RoboSep™ Rat IgM Depletion Kit, For processing: 1 x 10^9 cells
The EasySep™ Rat IgM Depletion Kit is designed to deplete IgM+ cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by positive selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
STEMCELL Technologies RoboSep™ Human CD2 Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human CD2 Positive Selection Kit II is designed to isolate CD2+ cells from fresh or previously frozen human peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD2 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD2 antigen is expressed on T cells and NK cells.This product replaces the EasySep™ Human CD2 Positive Selection Kit (Catalog #18657) for even faster cell isolations.
STEMCELL Technologies EasySep™ Mouse CD4+CD62L+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
Isolate highly purified naïve CD4+ T cells from single-cell suspensions of splenocytes or other tissues by using a simple, two-step method. First, naive CD4+ T cells are pre-enriched by negative selection using the EasySep™ Mouse Naïve CD4+ T Cell Pre-Enrichment Cocktail containing biotinylated antibodies directed against non-naïve CD4+ T cells (CD8, CD11b, CD11c, CD19, CD24, CD25, CD45R, CD49b, TCRγ/δ, TER119). Then CD4+CD62L+ cells, pre-labeled with CD62L PE, are selected using the EasySep™ PE Selection Cocktail. The desired cells are labeled with antibodies and magnetic particles. The cells are separated without columns using an EasySep™ magnet.For the isolation of mouse naïve CD4+ T cells in as little as 15 minutes by negative selection, we recommend using the EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (19765).
STEMCELL Technologies RoboSep™ Human B Cell Enrichment Kit II without CD43 Depletion, For processing: 1 x 10^9 cells
The EasySep™ Human B Cell Enrichment Kit II without CD43 Depletion is designed to isolate B cells by negative selection from fresh or previously frozen PBMCs of individuals with B cell leukemia or lymphoma, or other disease states in which B cells may express CD43. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.This kit replaces the EasySep™ Human B Cell Enrichment Kit without CD43 (Catalog #19154) for even faster cell isolations.
STEMCELL Technologies EasySep™ HLA B Cell Enrichment Kit, For processing: 1 x 10^9 cells
The EasySep™ HLA B Cell Enrichment Kit is designed to isolate B cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-B cells and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet and without the use of columns. Desired cells are poured off into a new tube and are ready for serology or flow cytometry crossmatch assays.
STEMCELL Technologies EasySep™ Human CD34 Positive Selection Kit II, For processing: 5 x 10^9 cells
The EasySep™ Human CD34 Positive Selection Kit II is designed to isolate CD34+ cells from fresh or previously frozen bone marrow or mobilized peripheral blood mononuclear cells, or from previously frozen cord blood mononuclear cells, or from hESC/hiPSC-derived hematopoietic cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD34 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD34 antigen is expressed on hematopoietic stem and progenitor cells.This product can be used in place of the EasySep™ Human CD34 Positive Selection Kit (Catalog #18056) for even faster cell isolations.
Mayflower Bioscience Exo-spin 20 mini-HD columns
Size exclusion chromatography (SEC) columns are a reliable method for exosome isolation and can be used as a stand-alone product or as part of a combined strategy for isolating small EVs. The EX05 Exo-spin™ kit contains packed and equilibrated ready-to-use SEC columns.
Hampton Research STOCKOPT ADA #15 PH 7.0
HR2-255-15/StockOptions ADA #15, 1.0 M ADA pH 7.0, 185 ml. Please note items from this supplier are non-returnable.
STEMCELL Technologies EasySep™ Release Human CD3 Positive Selection Kit, 1 x 10^9 cells
EasySep™ Release Human CD3 Positive Selection Kit, 1 x 10^9 cells
STEMCELL Technologies RoboSep™ FITC Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ FITC Positive Selection Kit II is designed to isolate cells that are labeled with FITC-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing FITC and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ FITC Positive Selection Kit (Catalog #18558) for even faster cell isolations.
STEMCELL Technologies RoboSep™ Mouse Plasmacytoid DC Isolation Kit, For processing: 2 x 10^9 cells
The EasySep™ Mouse Plasmacytoid DC Isolation Kit is designed to isolate plasmacytoid dendritic cells (pDCs) from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies that are directed against non-pDCs. Labeled cells are then recognized by Tetrameric Antibody Complexes that are directed against biotin and dextran. These cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
STEMCELL Technologies EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells from fresh peripheral blood mononuclear cells by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.This kit has been verified for use with rhesus and cynomolgus macaques.
STEMCELL Technologies RoboSep™ Human Gamma/Delta T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Gamma/Delta T Cell Isolation Kit is designed to isolate gamma/delta T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-gamma/delta T cells and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. The desired cells are poured off into a new tube. To obtain a custom kit optimized for culture-expanded PBMCs, contact STEMCELL Technologies’ Technical Support at techsupport@stemcell.com.