Protein Purification Reagents and Kits
Biotium Primary Antibody 6X His Tag 2405.H6 HRP 1/EA
This recombinant monoclonal mouse anti-6X His tag with HRP This HRP antibody conjugate has been validated for immunofluorescence and near-IR western blot
Zymo Research Corporation ChIP DNA Clean & Concentrator™ Kit (50 Preps) w/ Zymo-Spin™ I Columns (Uncapped)
The Chromatin Immunoprecipitation (ChIP) DNA Clean & Concentrator™ provides a hassle-free method for the rapid purification and concentration of high quality DNA from any step in a standard ChIP protocol.
Cayman Chemical Soluble Epoxide Hydrolase Inh
Cayman’s fluorescence-based sEH Inhibitor Screening Assay Kit provides a convenient method for screening human sEH inhibitors. The assay utilizes (3-phenyl-oxiranyl)-acetic acid cyano-(6-methoxy-naphthalen-2-yl)-methyl ester (PHOME) as a substrate. When the epoxide moiety of PHOME is hydrolyzed by sEH, an intramolecular cyclization occurs, which results in the release of a cyanohydrin under basic conditions. The cyanohydrin quickly decomposes into cyanide ion and the highly fluorescent 6-methoxy-2-naphthaldehyde,{14265} which can be analyzed using a fluorescence plate reader at excitation and emission wavelengths of 330 and 465 nm, respectively.
Abcam Cyclic Pifithrin-alpha hydrobromide, pifithrin-alpha analog, 10MG
MW 349.3 Da, Purity >98%. Stable, cell-permeable analog of pifithrin-α (ab120478). Inhibits p53-mediated apoptosis and p53-dependent gene transcription. Prevents dexamethasone-induced cell death in murine thymocytes (EC₅₀ = 2.01 μM). Potent STAT6 transcriptional inhibitor. Active in vivo and in vitro. See similar compound
The product is subject to the following: Abcam Restricted Use Statement
Abcam Donepezil hydrochloride, Acetylcholinesterase inhibitor, 10MG
MW 415.96 g/mol, Purity >99%. Potent, selective acetylcholinesterase inhibitor (IC₅₀ = 5.7 nM). Displays >1250 times selectivity for AChE over butyrylcholinesterase. Centrally active. Neuroprotective activity in vitro and in vivo.
The product is subject to the following: Abcam Restricted Use Statement
Biotium CD106 / VCAM1(B-K9), CF488A conjugate, 0.1mg/mL
CD106 (also known as vascular cell adhesion molecule-1, or VCAM-1) is a member of the Ig superfamily of adhesion molecules. It is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte beta-1 integrin VLA-4 and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Abcam Ciglitazone, PPARgamma agonist, 50MG
MW 333.4 Da, Purity >98%. Selective PPARγ agonist (EC₅₀ = 3 μM). Shows antidiabetic and antineoplastic activity. Induces cell cycle arrest and apoptosis. Active in vivo.
The product is subject to the following: Abcam Restricted Use Statement
AdipoGen DNER ED msFc h rec
Protein. Signal peptide and extracellular domain of mouse DNER aa 1-640 are fused at the C-terminus to the Fc portion of human IgG. Source HEK 293 cells. Endotoxin content <0.1EU/µg purified protein LAL test Lonza. Liquid. 0.2µm-filtered solution in PBS. Purity >85% SDS-PAGE. DNER is an activator of the Notch1 pathway. It mediates neuron-glia interaction during astrocytogenesis.
STEMCELL Technologies EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit, For processing: 20 mL biofluid processed
The EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit isolates highly purified human extracellular vesicles from plasma/serum and cell culture conditioned medium by immunomagnetic positive selection. Extracellular vesicles are labeled with Tetrameric Antibody Complexes recognizing CD9 and magnetic particles. Labeled extracellular vesicles are separated without columns using an EasySep™ magnet. Unwanted biofluid components are simply poured off, while desired extracellular vesicles remain in the tube. Isolated extracellular vesicles are available for downstream applications such as DNA/RNA extraction, Western blot, or mass spectometry.
STEMCELL Technologies RoboSep™ Human Naïve CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Naïve CD4+ T Cell Isolation Kit is designed to isolate naive CD4+ T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes and dextran-coated magnetic particles. CD45RO+ cells are targeted for removal with a biotinylated anti-CD45RO antibody, and a bispecific Tetrameric Antibody Complex that recognizes biotin and dextran. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.For even faster cell isolations, we recommend the new EasySep™ Human Naïve CD4+ T Cell Isolation Kit II (17555) (https://www.stemcell.com/products/easysep-human-naive-cd4-t-cell-isolation-kit-ii.html) which isolates cells in just 11 minutes.
STEMCELL Technologies RoboSep™ Human Resting CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Resting CD4+ T Cell Isolation Kit is designed to isolate resting CD4+ T cells from fresh or previously frozen human peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
STEMCELL Technologies RoboSep™ Human Biotin Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human Biotin Positive Selection Kit II is designed to isolate cells that are labeled with biotinylated antibodies. Desired cells are targeted with antibody complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Human Biotin Positive Selection Kit (Catalog #18553). For highly purified particle-free cells, we recommend the EasySep™ Release Human Biotin Positive Selection Kit (Catalog #17653). (https://www.stemcell.com/products/easysep-release-human-biotin-positive-selection-kit.html)
STEMCELL Technologies RoboSep™ Human Monocyte Enrichment Kit without CD16 Depletion with Filter Tips, For processing: 1 x 10^9 cells
The EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion is designed to isolate monocytes and CD14+CD16+ monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection. The CD14+CD16+ subset of monocytes (~10% in blood of healthy individuals) has characteristics of tissue macrophages and expands greatly in acute and chronic inflammatory disease. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-monocyte cells and magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For applications in which removal of all CD16+ cells is desired, we recommend the EasySep™ Human Monocyte Enrichment Kit (/products/easysep-human-monocyte-isolation-kit.html) (Catalog #19359), which contains anti-CD16.
STEMCELL Technologies EasySep™ Human TCR Alpha/Beta Depletion Kit, undefined: 1 Kit
The EasySep™ Human TCR Alpha/Beta Depletion Kit is designed to deplete human T cell receptor alpha/beta+ (TCRab+) cells from leukapheresis samples or expanded TCRa/b knockout cell cultures. TCRab+ cells are targeted with antibody complexes and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Labeled cells remain in the tube while unlabeled cells are poured off.
Cayman Chemical MK2 InhIbItr III 500ug
A potent, cell-permeable inhibitor of MK2 (IC50 = 8.5 nM); less potently blocks MK3 and MK5 (IC50s = 210 and 81 nM, respectively) and is weak or inactive against several other kinases, including other p38 MAP kinase targets; prevents LPS-induced synthesis of TNF-α in human monocyte-like U937 cells (IC50 = 4.4 µM)