Protein Purification Reagents and Kits
STEMCELL Technologies RoboSep™ Mouse PE Positive Selection Kit II, For processing: 5 x 10^9 cells
The EasySep™ Mouse PE Positive Selection Kit II is designed to isolate cells that are labeled with PE-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing PE and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This kit replaces the EasySep™ Mouse PE Positive Selection Kit (Catalog #18554).For highly purified particle-free cells, we recommend the EasySep™ Release Mouse PE Positive Selection Kit (Catalog #17656). (https://www.stemcell.com/products/easysep-release-mouse-pe-positive-selection-kit.html)
STEMCELL Technologies RoboSep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Kit, For processing: 60 mL buffy coat or whole blood processed
The EasySep™ Human Whole Blood Lymphoid Positive Selection Kit is designed to isolate CD3+ and CD19+ cells from whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD3, CD19 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD3 antigen is expressed on T lymphocytes and NKT cells while the CD19 antigen is expressed on B cells and follicular dendritic cells.This product replaces the EasySep™ Human Whole Blood Lymphoid Positive Selection Kit (Catalog #18684) and EasySep™ HLA Whole Blood Lymphoid Positive Selection Kit (Catalog #18684HLA) for even faster cell isolations.
STEMCELL Technologies EasySep™ HLA Whole Blood T Cell Enrichment Kit, For processing: 200 mL whole blood processed
The Complete Processing Kit for whole blood uses EasySep™ HLA T Cell Enrichment Kit to isolate human T cells from HetaSep™-treated whole blood by negative selection. Unwanted cells are targeted for removal with tetrameric antibody complexes recognizing non-T cells and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
STEMCELL Technologies RoboSep™ Human Naïve B Cell Enrichment Kit with Filter Tips, For processing: 1 x 10^9 cells
The EasySep™ Human Naïve B Cell Enrichment Kit is designed to isolate naïve B cells from peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-naïve B cells and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. The desired cells are poured off into a new tube.
STEMCELL Technologies EasySep™ Direct Human T Cell Isolation Kit for RoboSep™, For processing: 100 mL whole blood processed
This cell separation kit can be used to isolate highly purified T cells directly from human whole blood by immunomagnetic negative selection.This kit targets non-T cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and can directly be used for functional assays, flow cytometry or DNA/RNA extraction.
STEMCELL Technologies RoboSep™ Human APC Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human APC Positive Selection Kit II is designed to isolate cells that are labeled with APC (allophycocyanin)-conjugated antibodies by positive selection. Desired cells are targeted with antibody complexes recognizing APC and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Human APC Positive Selection Kit (Catalog #18451).
STEMCELL Technologies RoboSep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit, For processing: 60 mL whole blood processed
The EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection is designed to isolate CD4+ cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD4 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD4 antigen is expressed on helper T cells and more weakly on monocytes and tissue macrophages.This product replaces the EasySep™ Human Whole Blood CD4 Positive Selection Kit (Catalog #18082) for even faster cell isolations.
STEMCELL Technologies RoboSep™ Mouse PE Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Mouse PE Positive Selection Kit II is designed to isolate cells that are labeled with PE-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing PE and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This kit replaces the EasySep™ Mouse PE Positive Selection Kit (Catalog #18554).For highly purified particle-free cells, we recommend the EasySep™ Release Mouse PE Positive Selection Kit (Catalog #17656). (https://www.stemcell.com/products/easysep-release-mouse-pe-positive-selection-kit.html)
STEMCELL Technologies RoboSep™ Human Basophil Isolation Kit, For isolating: 1 x 10^9 cells
The EasySep™ Human Basophil Isolation Kit is designed to isolate basophils from a polymorphonuclear cell-rich fraction by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-basophils and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. The isolation kit is compatible with cells prepared using HetaSep™ (Catalog #07906) sedimentation of fresh peripheral blood.This product replaces the EasySep™ Human Basophil Enrichment Kit (Catalog #19069) for even faster cell isolations.
STEMCELL Technologies RoboSep™ Human Monocyte Enrichment Kit without CD16 Depletion with Filter Tips, For processing: 1 x 10^9 cells
The EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion is designed to isolate monocytes and CD14+CD16+ monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection. The CD14+CD16+ subset of monocytes (~10% in blood of healthy individuals) has characteristics of tissue macrophages and expands greatly in acute and chronic inflammatory disease. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-monocyte cells and magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For applications in which removal of all CD16+ cells is desired, we recommend the EasySep™ Human Monocyte Enrichment Kit (/products/easysep-human-monocyte-isolation-kit.html) (Catalog #19359), which contains anti-CD16.
STEMCELL Technologies EasySep™ Human TCR Alpha/Beta Depletion Kit, undefined: 1 Kit
The EasySep™ Human TCR Alpha/Beta Depletion Kit is designed to deplete human T cell receptor alpha/beta+ (TCRab+) cells from leukapheresis samples or expanded TCRa/b knockout cell cultures. TCRab+ cells are targeted with antibody complexes and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Labeled cells remain in the tube while unlabeled cells are poured off.
STEMCELL Technologies RoboSep™ Human Neutrophil Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Neutrophil Isolation Kit is designed to isolate neutrophils from a polymorphonuclear cell-rich fraction of peripheral blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-neutrophils and magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. The isolation kit is compatible with cells prepared using HetaSep™ (Catalog #07906) sedimentation or red blood cell lysis.This product replaces the EasySep™ Human Neutrophil Enrichment Kit (Catalog #19257), for even faster cell isolations.
STEMCELL Technologies RoboSep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit, For processing: 30 mL buffy coat processed
The EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit is designed to isolate CD14+ cells from freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD14 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD14 antigen is strongly expressed on monocytes and is weakly expressed on granulocytes. It is also expressed on most tissue macrophages.This product replaces the EasySep™ Human Buffy Coat CD14 Positive Selection Kit (Catalog #18088) for even faster cell isolations.
STEMCELL Technologies RoboSep™ Mouse CD8+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse CD8+ T Cell Isolation Kit is designed to isolate CD8+ T cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-CD8+ T cells and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.This product replaces the EasySep™ Mouse CD8+ T Cell Enrichment Kit (Catalog #19753) for even faster cell isolations.
Biotium HCG-intact(HCGab/52), CF647 conjugate, 0.1mg/mL
This MAb is a very special because it reacts ONLY with the intact-HCG and not with either free alpha- or free beta-chain of HCG. HCG is a glycoprotein and is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.Primary antibodies are available purified, or with a selection of fluorescent CF
